Identification method for fast-growing milkvetch root and wild milkvetch root

A technology of wild Astragalus membranaceus and Astragalus membranaceus, which is applied in the field of identification of Astragalus membranaceus germplasm resources, can solve the problems of limited collection and low specificity, and achieve the effects of high detection sensitivity and simple and easy-to-operate identification

Inactive Publication Date: 2016-11-09
SHANXI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, at present, researchers mostly use ultraviolet spectrophotometry to determine the content of total polysaccharides. This method has low specificity, which limits the inclusion of this index in the Chinese Pharmacopoeia.

Method used

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  • Identification method for fast-growing milkvetch root and wild milkvetch root
  • Identification method for fast-growing milkvetch root and wild milkvetch root
  • Identification method for fast-growing milkvetch root and wild milkvetch root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 6 samples each of the dried fast-growing Astragalus mongolica and the wild Astragalus mongolica, and grind them into powder respectively for later use;

[0025]Add each of the above sample powders to PBS buffer respectively, the mass volume ratio of each sample powder to PBS buffer is 1:30 (g / mL), place in a water bath at 42°C for about 1 hour; centrifuge at 5000 rpm, Discard the supernatant; repeat 2 times;

[0026] The above precipitate was extracted by adding 80% ethanol, the mass volume ratio of the precipitate to 80% ethanol was 1:30 (g / mL), placed in a water bath at 42°C for about 1 hour; centrifuged at 5000 rpm, discarded the supernatant; repeated the above extraction process Add acetone to the obtained precipitate, the mass volume ratio of the precipitate to acetone is 1:10 (g / mL), centrifuge at 5000 rpm, discard the supernatant; dry in an oven at 45°C;

[0027] The above precipitation was added to the porcine pancreatic α-amylase Tris-HCl buffer solution ...

Embodiment 2

[0039] Take 6 samples of dried fast-growing and wild Astragalus membranaceus, grind them into powder, and set aside; the fractional extraction of pectin polysaccharides and hemicellulose polysaccharides and the analysis of monosaccharide composition are the same as in Example 1. The results of the analysis can be seen in Table 2 and figure 1 , 2 , 3 and 4.

[0040] Table 2 The molar ratio of different sugar components in the cell wall of Astragalus membranaceus in different growth modes

[0041]

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Abstract

The invention discloses an identification method for fast-growing milkvetch root and wild milkvetch root. The identification method comprises: drying milkvetch root, and crushing; sequentially removing water-soluble components, fat-soluble components and starch from the milkvetch root powder by using a PBS buffer liquid, 80% ethanol, acetone and amylase; carrying out graded mixing on the treated milkvetch root powder sequentially by using a EPG solution, a NaCO3 solution, a 1 M KOH solution and a 4 M KOH solution to extract milkvetch root pectin polysaccharides and hemicellulose polysaccharides; sequentially washing the extracted pectin polysaccharides and the extracted hemicellulose polysaccharides, removing impurities, and carrying out free drying to obtain polysaccharide powder; and carrying out acid hydrolysis, acetylation and GC-MS analysis, and treating the data by using SMICA13 and SPSS software to obtain the molar ratio of the arabinose in the hemicellulose polysaccharide to the arabinose in the pectin polysaccharide, wherein the identified milkvetch root is the fast-growing milkvetch root when the ratio is more than 0.5, and the identified milkvetch root is the wild milkvetch root when the ratio is less than 0.5. According to the present invention, the detection sensitivity of the method is high, the polysaccharide component ratio identification is simple and is easy to operate, and the basis is provided for the identification of the milkvetch root herb source.

Description

technical field [0001] The invention relates to a method for identifying germplasm resources of Astragalus membranaceus, and specifically belongs to a method for identifying fast-growing Astragalus membranaceus and wild Astragalus membranaceus based on gas chromatography mass spectrometry technology and taking the ratio of monosaccharide in Astragalus membranaceus cell wall as the identification basis. Background technique [0002] Astragalus is one of the important bulk medicinal materials in my country. It has been used for more than 2,000 years. It was first recorded in "Shen Nong's Materia Medica" and listed as the top grade. Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao) or Astragalus membranaceus (A.membranaceus (Fisch.) Dried roots of Bge.). [0003] The traditional wild Astragalus mainly refers to Mongolian Astragalus or Astragalus membranaceus that grow naturally after more than 5 years of growth, natural reproduction or artificial seeding. Wil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/025
Inventor 李科郝霞王桂臻刘磊秦雪梅
Owner SHANXI UNIV
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