Homogeneous pectic polysaccharides and method for obtaining same from tea polysaccharides

A technology of pectin polysaccharides and tea polysaccharides, applied in the field of polygalacturonic acid homogeneous pectin polysaccharides, can solve the problems of destroying the active structure of tea polysaccharides, reducing the content of uronic acid, and high cost

Inactive Publication Date: 2012-09-19
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] To sum up the content of the above research, it can be seen that the current research on tea polysaccharides is mainly on the acquisition and activity of tea polysaccharides. Among them, the methods for obtaining tea polysaccharides mainly include water extraction and alcohol precipitation, acid extraction, alkali extraction, enzymatic extraction; however, acid extraction and alkaline extraction can easily hydrolyze part of tea polysaccharides, thus destroying the active structure of tea polysaccharides, while enzymatic extraction not only has high cost but also reduces the content of uronic acid-containing parts. The yield of tea polysaccharides; and most of the disclosed purification techniques also involve toxic and harmful reagents
Although studies have reported that tea polysaccharides contain polygalacturonic acid pectin polysaccharides, how to separate and purify polygalacturonic acid pectin polysaccharides from tea polysaccharides has not seen any relevant technical reports so far

Method used

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  • Homogeneous pectic polysaccharides and method for obtaining same from tea polysaccharides
  • Homogeneous pectic polysaccharides and method for obtaining same from tea polysaccharides
  • Homogeneous pectic polysaccharides and method for obtaining same from tea polysaccharides

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Experimental program
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Effect test

Embodiment 1

[0104] Embodiment 1: Preparation of uniform pectin polysaccharide TPS4-1B

[0105] 11) Grinding the dried tea leaves to 40-100 meshes;

[0106] 12) Extract twice with water at 100°C. The volume of water added for the first time is 15 times the mass of tea leaves, and the volume of water added for the second time is 10 times the mass of tea leaves; the secondary extracts are combined, concentrated, and ethanol is added for alcohol precipitation to control alcohol The concentration of ethanol in the precipitation system is 40vol%; after alcohol precipitation for 24 hours, filter and collect the precipitate; centrifuge and freeze-dry the precipitate to obtain the crude tea polysaccharide;

[0107] 13) Put the crude tea polysaccharide obtained in step 12) on the DEAE-Sepharose column, and then elute with water and 0.1mol / L sodium chloride aqueous solution; use the sulfuric acid-phenol method to track the elution curve, collect and utilize 0.1mol / L The effective part that the sodi...

Embodiment 2

[0147] Embodiment 2: Preparation of uniform pectin polysaccharide TPS4-2A

[0148] 21) Grinding the dried tea leaves to 40-100 meshes;

[0149] 22) Extract twice with water at 100°C. The volume of water added for the first time is 15 times the mass of tea leaves, and the volume of water added for the second time is 10 times the mass of tea leaves; the secondary extracts are combined, concentrated, and ethanol is added for alcohol precipitation to control alcohol The concentration of ethanol in the precipitation system is 40vol%; alcohol precipitation? Hours later, filter and collect the precipitate; centrifuge and freeze-dry the precipitate to obtain crude tea polysaccharide;

[0150] 23) Put the crude tea polysaccharide obtained in step 22) on a DEAE-Sepharose column, and then elute with water, 0.1mol / L aqueous sodium chloride solution and 0.2mol / L sodium chloride aqueous solution; use the sulfuric acid-phenol method Track the elution curve, and collect the effective parts ...

Embodiment 3

[0162] Embodiment 3: Preparation of uniform pectin polysaccharide TPS7-1B

[0163] 31) Grinding the dried tea leaves to 40-100 meshes;

[0164] 32) Extract twice with water at 100°C. The volume of water added for the first time is 15 times the mass of tea leaves, and the volume of water added for the second time is 10 times the mass of tea leaves; the secondary extracts are combined, concentrated, and ethanol is added for alcohol precipitation to control alcohol The ethanol concentration in the precipitation system is 70vol%; after 24 hours of alcohol precipitation, filter and collect the precipitate; centrifuge and freeze-dry the precipitate to obtain the crude tea polysaccharide;

[0165] 33) Put the crude tea polysaccharide obtained in step 32) on a DEAE-Sepharose column, and then elute with water and 0.1mol / L sodium chloride aqueous solution; use the sulfuric acid-phenol method to track the elution curve, collect and utilize 0.1mol / L The effective part that the sodium chl...

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Abstract

The invention discloses homogeneous pectic polysaccharides and a method for obtaining the same from tea polysaccharides. In particular, the invention relates to polygalacturonic acid homogeneous pectic polysaccharides and a method for obtaining the same from tea polysaccharides or tea leaf polysaccharides. The homogeneous pectic polysaccharides and the method have the following beneficial effects: 1,4-connected polygalacturonic acid pectic polysaccharides are obtained from tea polysaccharides through separation and purification for the first time; the preparation method is simple and is high in yield; acids, alkalies and other toxic and harmful reagents are not used in the whole preparation process, thus the stability of the activity structures of tea polysaccharides can be fully ensured and the preparation method is suitable for large-scale production; in particular, the tea leaves obtained after extracting tea polyphenols can be effectively utilized by using the method, thus the resources can be saved and the refuses can be reclaimed; and the method has great significance in study and production of polygalacturonic acid homogeneous pectic polysaccharides.

Description

technical field [0001] The invention relates to a method for obtaining uniform pectin polysaccharides from tea polysaccharides and the obtained uniform pectin polysaccharides, in particular to a method for obtaining polygalacturonic acid homogeneous pectin polysaccharides from tea polysaccharides or tea dregs polysaccharides The method and the obtained polygalacturonic acid homogeneous pectin polysaccharide. Background technique [0002] Tea leaves are the buds and leaves of the Theaceae plant. "Shen Nong's Materia Medica" records that "tea is bitter and cold, and the yin in the yin is also sinking, and it can best reduce fire." It has been widely used by the people. Modern medicine has proved that tea has the pharmacological effects of cooling, detoxifying, quenching thirst, diuretic, refreshing, eliminating fat and greasy, refreshing the mind, prolonging life and so on. With the advancement of medical science and technology, in-depth research on tea ingredients and medi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/06
Inventor 王顺春王辉俊鲍斌施松善王峥涛胡之璧
Owner SHANGHAI UNIV OF T C M
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