Pectic polysaccharides isolated from fruit pods of okra

A technology of okra fruit and pectin, applied in skin diseases, drug combination and other directions, can solve problems such as incomplete metabolism and inflammatory reaction

Inactive Publication Date: 2009-05-27
BOHUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, one problem when HPMC is used as an ophthalmic viscoelastic surgical device is that it is not fully metabolized in the anterior chamber.
In addition, plant fibers in HPMCs may contribute to inflammatory responses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0055] A medical composition containing pectin-like polysaccharide and water can be easily prepared. It may also contain physiological buffers.

[0056] In addition, the medicinal composition may contain 0.1-20% pectin-like polysaccharide. It can have a greater than 1.0m 3 / kg intrinsic viscosity η (corresponding to 1.0×10 6 Da molecular weight).

[0057] Furthermore, the pectin-like polysaccharides described above can be used in the manufacture of pharmaceutical compositions for the surgical treatment of any ophthalmic disease. This eye disease can be cataract.

[0058] The medical composition can also be used as an ophthalmic viscoelastic surgical device (OVD). The high viscosity and high purity of pectin-like polysaccharides, and the fact that pectin-like polysaccharides do not easily cause inflammatory reactions in patients since they are not of animal origin, make pectin-like polysaccharides suitable for use as OVDs.

[0059] Furthermore, pectin-like polysaccharides...

Embodiment 1

[0089] Cut the okra pods into approximately 2mm slices. Add 5 liters of 0.5M NaOH to pure water. Mix with slow stirring at room temperature (20°) at pH 11 for 24 hours. The remaining fruit tissue was separated from the extract by filtering through a nylon mesh. The extract is stored in a refrigerator at a temperature of 4-8°C.

[0090] The content of pectin-like polysaccharide is 56.23%, the content of protein is 0.016%, and its intrinsic viscosity is 3.40m 3 / kg.

[0091] A second extraction was performed using the same okra fruit material. Add 3 liters of purified water, pH 11, to the fruit tissue. The extraction time was 24h under slow stirring at room temperature. The extract thus formed is completely processed into the first extract. Its pectin-like polysaccharide content is 51.68%, protein content 3 / kg.

[0092] The third extraction was performed in exactly the same way as the second extraction. Its pectin content is 47.56%, protein content 3 / kg.

[0093] The...

Embodiment 2

[0098] Example 2 was carried out according to the method described in Example 1, except for the pH during extraction. Its pH was adjusted to 4-5 by acetic acid.

[0099] The total dry weight of the fibers was 9.773 g. Finally, the content of pectin-like polysaccharide analyzed in the fiber was 57.05%, the content of protein was 0.36mg / g and the intrinsic viscosity was 3.40m 3 / kg.

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PUM

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Abstract

The invention relates to a method for producing a high molecular weight pectin-like polysaccharide from fruit pods of okra. The high molecular weight pectin-like polysaccharide, when dissolved in a buffer, exhibit viscoelastic properties, and is very useful in applications such as ophthalmic surgery, dermatology and orthopedics.

Description

field of invention [0001] The present invention relates to a process for the preparation of high molecular weight polysaccharides. The invention further relates to the use of said polysaccharides in ophthalmic surgery, dermatology and orthopedics. Background of the invention [0002] Viscoelastic surgery is a term generally used to describe surgical procedures in which viscoelastic fluids (or viscoelastic substances) are used as surgical instruments or implants to protect, manipulate and separate diseased tissue to aid in surgical procedures. The term viscoelastic substances is very broad and includes substances used in applications such as ophthalmology, dermatology and orthopedics. [0003] Ophthalmic viscosurgical devices (OVD) are generally the preferred term for viscoelastic fluids in ophthalmology (Arshinoff S., "New terminology: Ophthalmic viscosurgical devices", J. Cataract Refract Surg (2000) 26:627-628). OVDs are commonly used in a number of different eye procedu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C08B37/06
CPCC08B37/006C08B37/0045A61P17/00
Inventor A·黑沃尔J·卡尔德隆维拉D·奥格博纳亚
Owner BOHUS BIOTECH
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