Method for preparing RG-I pectic polysaccharide rich in arabinose side chains

A pectin polysaccharide, RG-I technology, applied in the field of pectin extraction, can solve the problems of insufficient source of RG-I pectin raw materials, complex extraction process, small storage capacity, etc., to achieve low cost, simple process, and short production cycle short effect

Active Publication Date: 2021-07-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have shown that the raw material sources of RG-I pectin rich in arabinose are not wide enough and the storage capacity is small, and its extraction process is complicated and the yield is low.

Method used

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  • Method for preparing RG-I pectic polysaccharide rich in arabinose side chains
  • Method for preparing RG-I pectic polysaccharide rich in arabinose side chains
  • Method for preparing RG-I pectic polysaccharide rich in arabinose side chains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Peanut wet residue was provided by a peanut oil processing factory in Jiangsu and kept frozen. Take 1kg of wet-based material, add 1.8L of deionized water, melt, stir evenly, adjust the pH of the whole system to 5.0 with 20% citric acid solution, place it in a high-pressure reactor at 100°C, fill it with nitrogen, stir, and adjust the system pressure 5.0Mpa, react for 40min. After the reaction is finished, cool to room temperature, centrifuge at 9000rpm for 15min to obtain the supernatant, and use a 100,000 Dalton ultrafiltration membrane to conduct ultrafiltration separation of the above crude extract at a temperature of 35°C and a pressure of 15Psi for constant volume ultrafiltration, and the retentate Freeze-dried to obtain 25g of refined peanut residue RG-I pectin polysaccharide, the yield of this component reached 37.87%, and the purity of RG-I was 90%.

[0051] Pectin monosaccharide composition analysis was performed by high performance anion exchange chromatogra...

Embodiment 2

[0060] Peanut wet residue was provided by a peanut oil processing factory in Jiangsu and kept frozen. Take 2kg and add 3.6L deionized water, stir evenly, adjust the pH of the whole system to 3.9 with 20% citric acid solution, heat to 95°C, fill with nitrogen to keep the pressure at 5.5Mpa, and react for 40min. After the reaction is completed, cool to room temperature, centrifuge at 9000rpm for 15min, and obtain the supernatant. A 100,000 Dalton ultrafiltration membrane was used to conduct ultrafiltration and separation of the above crude extract at a temperature of 35° C. and a pressure of 20 Psi for constant volume ultrafiltration. The retentate was freeze-dried to obtain 66 g of refined peanut residue RG-I pectin polysaccharide. The yield of this component reaches 34.9%, and the purity of RG-I is 92.5%.

[0061] Determination of peanut pomace pectin polysaccharide according to the high-efficiency anion-exchange chromatography described above, its composition is as follows:...

Embodiment 3

[0068] Peanut wet residue was provided by a peanut oil processing factory in Jiangsu and kept frozen. Take 2.5kg of wet-based material, add 4.5L of deionized water, stir evenly, adjust the pH of the whole system to 4.5 with 20% citric acid solution, and then place it in a high-pressure reactor at 105°C

[0069] , filled with nitrogen, pressure 6.0Mpa, reaction 40min. After the reaction was completed, cool to room temperature, centrifuge at 9000rpm for 15min to obtain the supernatant, and use a 100,000 Dalton ultrafiltration membrane to conduct ultrafiltration separation of the above crude extract at a temperature of 35°C and a pressure of 25Psi for constant volume ultrafiltration. The retentate was freeze-dried to obtain 89 g of refined peanut residue RG-I pectin polysaccharide. The yield of this component relative to the dry basis reaches 30.2%, and the purity reaches 95.3%.

[0070] Determination of peanut pomace pectin polysaccharide according to the high-efficiency anion...

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Abstract

The invention discloses a method for preparing RG-I pectic polysaccharide rich in arabinose side chains, and belongs to the technical field of pectin extraction. The method comprises the following steps: by taking peanut meal as a raw material, carrying out medium-temperature and high-pressure treatment in an acidic and nitrogen environment, and centrifugally filtering to obtain pectic polysaccharide rich in RG-I; wherein the peanut meal is a by-product generated in the processing process of extracting oil from peanut oil by a water medium method; and the medium-temperature and high-pressure treatment conditions are as follows: the temperature is 90-105 DEG C, and the pressure is 2-7Mpa. The pectic polysaccharide rich in arabinose side chain RG-I is extracted from the peanut meal by adopting the method, the extraction rate reaches 35-40%, and the purity reaches 85-92%.

Description

technical field [0001] The invention relates to a method for preparing RG-I pectin polysaccharide rich in arabinose side chains, belonging to the technical field of pectin extraction. Background technique [0002] Pectin is a kind of heterogeneous polysaccharide with complex structure, which is an important component of plant cell wall, and widely exists in fruits, roots, stems and leaves of plants. At present, the structural characteristics of pectin are generally agreed that it is composed of homogalacturon region (Homogalacturon Region, HG region), rhamngalacturonan I (RG I), rhamnogalacturonan II (Rhamngalacturonan II, RG II) and xylogalacturonan (Xylogalacturonan, XG). Among them, RG I is the region with the most branched chains and the most heterogeneous structure on the pectin structure, which is called the "hair region". The main structure of RG-I is composed of repeating disaccharide unit α-4-D-galacturonic acid-(1-2)-α-rhamnose-1, and the hydroxyl group and oxyge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/06
CPCC08B37/0048
Inventor 杨瑞金叶建芬张文斌仝艳军李鹏飞
Owner JIANGNAN UNIV
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