Rice wine lees-derived antihypertensive oligopeptide, preparation method and application thereof

A technology of rice distiller's grains and blood pressure lowering, which is applied in the field of resource utilization of rice wine tanks and blood pressure reduction, can solve the problems of environmental pollution and waste of resources, and achieve the effect of good activity, high purity, and favorable resource utilization

Active Publication Date: 2020-08-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology makes use of rice by producing it for sale at home or restaurants where people want their drink without adding too much sugar due to concerns about health issues related to excessive consumption of alcoholic liquor made from this type of food called grapes.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the use of rice products with high concentrations of certain compounds called histone convertase inhibiting agents like captoprene during processing into useful substances while minimizing harmful chemicals released from these processes.

Method used

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  • Rice wine lees-derived antihypertensive oligopeptide, preparation method and application thereof
  • Rice wine lees-derived antihypertensive oligopeptide, preparation method and application thereof
  • Rice wine lees-derived antihypertensive oligopeptide, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1 Separation of blood pressure lowering peptides

[0032] (1) Materials and reagents

[0033] Material: The remaining base material (rice lees) from the rice wine fermentation of Shunde Hongli Winery.

[0034] Reagents: see Table 1 for details.

[0035] Table 1 Reagent table

[0036]

[0037] (2) Determination method of ACE inhibitory activity

[0038] 2.1 Preparation of the reaction solution

[0039] Add 10 μL ACE solution (0.25U / mL, ACE enzyme is dissolved in potassium phosphate buffer pH7) to the sample tube (B) and blank tube (A) respectively, add 10 μL ACEI to the sample tube, and 10 μL buffer to the blank tube. After incubation for 5 min, add 30 μL of HHL solution (6.5 mmol / mL, HHL dissolved in 0.1 mol·L-1 boric acid buffer with pH 8.3, containing 0.3 mol·L-1 NaCl), react at 37 °C for 1 h, and then add 80 μL of 1.0 mol / L HCl was used to stop the reaction, and a reaction solution was obtained, as shown in Table 2.

[0040] The preparation of table ...

experiment example 2

[0072] Experimental example 2 Synthesis of hypotensive oligopeptide and evaluation of ACE inhibitory activity

[0073] In order to carry out reverse verification of hypotensive oligopeptide H5 and characterize its inhibitory effect on ACE in vitro, high-purity hypotensive oligopeptide H5 (purity>98%) was synthesized by Shanghai Jietai Biotechnology Co., Ltd. The activities of the oligopeptides separated at each stage were compared, and the ACE inhibitory activity evaluation test was carried out according to the assay method of ACE inhibitory activity, and the IC obtained from the test was 50 The values ​​are shown in Table 6.

[0074] According to the results in Table 6, it can be seen that the hypotensive oligopeptide H5 isolated in the present invention has good ACE inhibitory activity, and it was reversely verified that this oligopeptide does have the effect of lowering blood pressure by means of artificial synthesis. But the IC of synthetic oligopeptide 50 The value is h...

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Abstract

The invention belongs to the technical field of rice wine lees resource utilization and blood pressure reduction. The invention relates to an antihypertensive oligopeptide, in particular to a rice wine lees-derived antihypertensive oligopeptide, a preparation method and application thereof. For promopting comprehensive utilization of the rice wine lees, the oligopeptide with the effect of reducingblood pressure is developed; rice wine lees are used as raw materials; separating and purifying to obtain the antihypertensive oligopeptide. The oligopeptide has a structure as shown in SEQ ID NO: 1.The amino acid sequence shown in the formula 1 has the molecular weight of 719.857 Da, is high in purity, good in activity, capable of being artificially synthesized, safe and non-toxic, and the ACEinhibitory activity measurement shows that the oligopeptide has a remarkable inhibitory effect on angiotensin converting enzyme in vitro, can be applied to the field of blood pressure reduction and isbeneficial to resource utilization of rice wine waste.

Description

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Claims

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Application Information

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Owner SOUTH CHINA AGRI UNIV
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