A kind of lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and Escherichia coli, which is applied in the direction of application, bacteria, and microorganisms, and can solve the problems of insufficient bile salt resistance and loss

Active Publication Date: 2018-01-09
BEIJING BOJINYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the bacteria are not resistant to acid and bile salts, they cannot enter the intestinal tract and lose their function

Method used

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  • A kind of lactobacillus plantarum and application thereof
  • A kind of lactobacillus plantarum and application thereof
  • A kind of lactobacillus plantarum and application thereof

Examples

Experimental program
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Embodiment Construction

[0021] An example is: this Lactobacillus plantarum, which is Lactobacillus plantarum (Lactobacillus plantarum) BJYL132, the preservation number is CGMCC No.9646, and the preservation date is September 11, 2014. The preservation unit: China Microbiological Culture Preservation Management Committee Common Microorganisms center.

[0022] The isolation and identification methods of Lactobacillus plantarum BJYL132 are as follows:

[0023] 1. Separation method

[0024] Weigh 5g of the sample (naturally fermented food) into 45ml of sterile buffered saline solution (with an appropriate amount of glass beads) as the mother solution, oscillate for 10 minutes to mix thoroughly, and make gradient dilution with sterile buffered saline solution, choose three suitable gradients, and draw Streak and separate 0.1ml in MRS medium, do two repetitions for each dilution gradient, place the culture dish in a 37°C incubator for 48-72 hours, and select smooth, convex, neat edges, white or milky whit...

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Abstract

The present invention relates to a plant lactobacillus and its application. The plant lactobacillus is a plant lactobacillus (Lactobacillus plantarum) BJYL132, the preservation number is: CGMCC No.9646, and the preservation date is September 11, 2014. Unit: General Microbiology Center of China Committee for Culture Collection of Microorganisms. The Lactobacillus plantarum BJYL132 is derived from naturally fermented food, can colonize human bodies and animals, and is resistant to gastric acid and bile salts; at the same time, the bacterium has a good inhibitory effect on Escherichia coli and can survive at room temperature for 30 Day; this strain has a better synergistic growth effect with other types of lactic acid bacteria, and can be used in E. coli antibacterial liquid, aquaculture, and can also be widely used in other food and biological products fields.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to a plant lactobacillus and application thereof. Background technique [0002] Lactobacillus plantarum is closely related to the lives of humans and animals. It is a lactic acid bacteria commonly found in cream, meat, many vegetable fermented products, and silage. It can pass through the stomach and colonize the intestinal tract to play a beneficial role, such as maintaining intestinal bacteria It has many functions, such as the balance of group, promoting the absorption of nutrients, alleviating lactose intolerance and inhibiting the formation of tumor cells. At present, there are many application fields including food, feed, medical care, cosmetics, beauty, aquaculture, industry and agriculture. production, biological preservatives, probiotic preparations, etc. [0003] Multiple physiological functions of Lactobacillus plantarum as a nitrogen source for the host. However,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K50/80A23K10/18C12R1/25
CPCC12N1/205C12R2001/25
Inventor 刘英华姜波
Owner BEIJING BOJINYUAN BIOTECH
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