A kind of lactobacillus plantarum and application thereof
A technology of Lactobacillus plantarum and Escherichia coli, which is applied in the direction of application, bacteria, and microorganisms, and can solve the problems of insufficient bile salt resistance and loss
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[0021] An example is: this Lactobacillus plantarum, which is Lactobacillus plantarum (Lactobacillus plantarum) BJYL132, the preservation number is CGMCC No.9646, and the preservation date is September 11, 2014. The preservation unit: China Microbiological Culture Preservation Management Committee Common Microorganisms center.
[0022] The isolation and identification methods of Lactobacillus plantarum BJYL132 are as follows:
[0023] 1. Separation method
[0024] Weigh 5g of the sample (naturally fermented food) into 45ml of sterile buffered saline solution (with an appropriate amount of glass beads) as the mother solution, oscillate for 10 minutes to mix thoroughly, and make gradient dilution with sterile buffered saline solution, choose three suitable gradients, and draw Streak and separate 0.1ml in MRS medium, do two repetitions for each dilution gradient, place the culture dish in a 37°C incubator for 48-72 hours, and select smooth, convex, neat edges, white or milky whit...
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