Microorganism water purification agent and preparation method thereof

A preparation process and microbial technology, applied in the field of microorganisms, can solve problems such as eutrophication and easy destruction of water quality, and achieve the effects of maintaining quality, better purification effect, and simple process flow

Active Publication Date: 2014-06-25
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of easy damage and eutrophication of water quality in aquaculture water, the present invention proposes a microbial water purification agent and its preparation process, using the agent to purify the quality of aquaculture water, making the water quality better and increasing the production of aquatic products

Method used

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  • Microorganism water purification agent and preparation method thereof
  • Microorganism water purification agent and preparation method thereof
  • Microorganism water purification agent and preparation method thereof

Examples

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Effect test

Embodiment 1

[0031] Select 1500ml of No. 1, 2, 3, 4, and 5 mediums in Table 1, respectively, and put each medium into a 25L fermenter, sterilize at 120°C for 20min, and inoculate after the medium cools down. The agent is a liquid bacterial agent containing 18.75ml of Bifidobacteria, 22.5ml of Candida tropicalis and 33.75ml of Bacillus subtilis, and the inoculum is 75ml. Initially, the pH in the fermenter was 6.8, the fermentation temperature was 30°C, anaerobic fermentation was carried out under the condition of no ventilation and the stirring speed was 250r / min, and 100ml samples were taken every 24 hours for the fermenter, and a total of 5 samples were taken, that is, each fermentation Tank fermentation stops after 120h.

Embodiment 2

[0033] Sugar cane molasses: 38.7ml sucrose, 10.8ml glucose, 13.5ml fructose, 3.6ml carbohydrates, 23.4ml water. Preparation of sugarcane molasses medium: sugarcane molasses 90ml, NH with a mass concentration of 1.0% 4 Cl7.5ml, KH with a mass concentration of 0.5% 2 PO 4 22.5ml, 1# trace element 7.5ml, purified water 1372.5ml. Measure 1500ml, put it into a 25L fermenter, sterilize at 100°C for 25min, inoculate after cooling the culture medium, the bacterial agent is 9ml of Bifidobacterium, 15.75ml of Candida tropicalis and 20.25ml of subtilis The liquid inoculum of Bacillus, the inoculum size is 45ml. Initially, the pH in the fermenter is 6.8, the fermentation temperature is 30°C, anaerobic fermentation is carried out for 72 hours under the condition of no ventilation and the stirring speed is 200r / min, and the microbial agent for purifying aquaculture water quality of the present invention is obtained.

Embodiment 3

[0035] Sugarcane molasses: 28.2ml sucrose, 4.8ml glucose, 11.4ml fructose, 1.2ml carbohydrate, 14.4ml water. Preparation of sugarcane molasses medium: take 60ml of sugarcane molasses and NH with a mass concentration of 2.0% by volume percentage 4 Cl37.5ml, KH with a mass concentration of 1.2% 2 PO 44.5ml, 1.5ml of 2# trace elements, 1396.5ml of distilled water. Measure 1500ml, put it into a 25L fermenter, sterilize at 130°C for 15min, inoculate after cooling the culture medium, the bacterial agent is 36ml of Bifidobacterium, 36ml of Candida tropicalis and 48ml of Bacillus subtilis The liquid bacterial agent, the inoculation volume is 120ml. Initially, the pH in the fermenter was 6.8. During the fermentation process, the fermentation temperature was 30° C., and the anaerobic fermentation was carried out for 72 hours without ventilation and the stirring speed was 300 r / min, and the bacterial agent for purifying aquaculture water quality of the present invention was obtained. ...

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Abstract

The invention relates to a microorganism water purification agent and a preparation method thereof. The preparation method comprises following specific steps of: inoculating the agent onto a cane molasses medium, and fermenting for 24-120 hours at 25-35DEG C so as to cultivate the agent. The method for purifying the water quality of aquaculture water by the prepared microorganism water purification agent comprises the step of adding 15-18ml of the agent prepared by the method into one cube of aquaculture water. According to the method, the cane molasses medium is used for cultivating the agent, so that the amount of viable bacteria can be increased to the greatest extent, the amount of viable bacteria of bacillus subtilis, which plays a main role in purifying the water body, can be increased, and the purification effect of the aquaculture water is better; for the fermentation time, the amount of viable bacteria of the agent can be improved, furthermore, and the agent holds certain amount of sugar so as to provide nutrient for the bacteria, thus being beneficial to guaranteeing the quality; the agent prepared by the method can effectively solve the problem of damage and eutrophication of the quality of the aquaculture water, thus improving the quantity and quality of aquatic products.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a microbial water purifying bacterial agent and a preparation process thereof. Background technique [0002] Microecologics (Microecological modulator) is a kind of biological preparation that uses carbohydrates and other substances as carbon donors and is fermented by beneficial bacteria. It contains a large number of beneficial bacteria and active substances such as oligosaccharides and biological enzymes. Select beneficial bacteria that can purify water quality, and the microbial preparations cultivated therefrom can purify water well. For eutrophic water bodies, such as closed or semi-closed water bodies such as lakes, rivers and bays, biological agents can be used for treatment. In aquaculture, it is often used to adjust water quality, eliminate harmful substances, and create good water for aquaculture. [0003] Sugarcane molasses is the main by-product of sugar production in s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 周宇黄凯沈桂宇武林华陆荣聂文衍红何金钊程远黄清黄秀芸唐丽宁
Owner GUANGXI UNIV
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