Bacillus subtilis liquid fermented licorice microecological preparation, preparation method and application as feed additive for livestock and poultry

A micro-ecological preparation, Bacillus subtilis technology, applied in the field of micro-ecological preparations, can solve problems such as poor effect, and achieve the effects of low production cost, promotion of increase, and promotion of spore formation.

Inactive Publication Date: 2018-04-27
河南省医药科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the probiotics of Bacillus subtilis commonly used in the market are mostly cultivated in ordinary medium, and added to feed or drinking water in the form of solid powder, which has poor effect

Method used

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  • Bacillus subtilis liquid fermented licorice microecological preparation, preparation method and application as feed additive for livestock and poultry
  • Bacillus subtilis liquid fermented licorice microecological preparation, preparation method and application as feed additive for livestock and poultry
  • Bacillus subtilis liquid fermented licorice microecological preparation, preparation method and application as feed additive for livestock and poultry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A Bacillus subtilis liquid fermented licorice micro-ecological preparation, which is obtained by inoculating Bacillus subtilis seed liquid into a licorice fermentation medium, and cultivating by shaking; the licorice fermentation medium formula is: licorice water extract 0.5mg / L, LB liquid medium 250mg / L, pH 7.4.

[0027] 5% licorice water extract, 0.25% LB liquid medium, the balance is water, pH 7.4.

[0028] The preparation method of the above-mentioned Bacillus subtilis liquid fermented licorice microecological preparation, it comprises the steps:

[0029] 1) Inoculate the Bacillus subtilis strain in LB liquid medium, shake and cultivate at 37°C for 48 hours to obtain the Bacillus subtilis seed liquid;

[0030] 2) Inoculate the Bacillus subtilis seed liquid into the licorice fermentation medium at an inoculum amount of 5% (v / v), and then shake and culture at 37°C for 24-72 hours.

Embodiment 2

[0032] In the Bacillus subtilis liquid fermented licorice microecological preparation, the licorice fermentation medium formula is: 1.0 mg / L of licorice water extract, 250 mg / L of LB liquid medium, and pH 7.4. Others and preparation methods are the same as in Example 1.

Embodiment 3

[0034] In the Bacillus subtilis liquid fermented licorice microecological preparation, the licorice fermentation medium formula is: 1.5 mg / L of licorice water extract, 250 mg / L of LB liquid medium, and pH 7.4. Others and preparation methods are the same as in Example 1.

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Abstract

The invention relates to a licorice microecological preparation fermented by Bacillus subtilis liquid, which is obtained by inoculating Bacillus subtilis seed liquid into licorice fermentation medium and culturing by shaking; the formula of the licorice fermentation medium is: licorice water extract 0.5-1.5mg / L, LB liquid medium 250mg / L, pH 7.4. The present invention utilizes Bacillus subtilis to ferment licorice in a liquid, so the production cost is low, and at the same time, it promotes the increase of the viable bacteria of Bacillus subtilis and the formation of spores, and after fermentation, it becomes a new type of microecological preparation, which is added to the drinking water of livestock and poultry to improve the digestibility of livestock and poultry, and the production performance and immunity.

Description

technical field [0001] The invention belongs to the technical field of microecological preparations, and in particular relates to a Bacillus subtilis liquid fermented licorice microecological preparation, a preparation method and applications thereof. Background technique [0002] Bacillus subtilis is a kind of Bacillus, which can form spores and is a Gram-positive bacteria. As a livestock and poultry feed additive, it can produce a variety of digestive enzymes, and has the effect of improving animal digestion and absorption, production performance and animal immune performance. At present, the commonly used Bacillus subtilis microecological preparations on the market are mostly cultivated in ordinary medium and added to feed or drinking water in the form of solid powder, and the effect is not good. SUMMARY OF THE INVENTION [0003] The purpose of the present invention is to overcome the defects of the prior art, and to provide a Bacillus subtilis liquid fermented licorice...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/30A23K50/30C12N1/20C12N1/04C12R1/125
Inventor 郑立运方先珍乔宏兴王宁赵志鸿张壮丽戈士文马芳王庆端
Owner 河南省医药科学研究院
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