Method for preparing vector substrate of plant growth promoting bacterium agent from green straws

A technology of plant growth-promoting bacteria and carriers, which is applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc. Eliminates problems such as body death, and achieves high viable bacterial count, good bacterial loading effect, low pH value change and low pollution rate

Inactive Publication Date: 2014-06-04
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Excessive sugar and nitrogen sources will lead to excessive reproduction of bacteria and excessive accumulation of bacterial metabolites, which will change the pH value of the bacteria agent and cause the death of a large number of bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for preparing plant growth-promoting bacterium agent carrier matrix with green straw, comprises the steps:

[0026] (a) Raw material processing: Collect corn stalks with green leaves within 48 hours after harvesting ears. At this time, corn stalks with green leaves contain about 0.89% of total nitrogen, 0.41% of phosphorus, 0.22% of Mg, K1.59%, and Ca0.64 %, dry the corn stalks with leafy greens in the shade, after drying in the shade, the moisture content in the corn stalks with green leaves is 7.29%, cut the stalks with green leaves into sections and crush them with a pulverizer, and pass through a sieve with an aperture of 0.02mm;

[0027] (b) Extraction of raw materials: leaching the sieved corn stalk powder with water at 60°C for 12 hours, wherein the weight ratio of the corn stalk powder to water is 1:25;

[0028] (c) Filtration and neutralization: filter after leaching, and adjust the pH of the filtrate to 6.7 with phosphate buffer;

[0029] (d) Dryin...

Embodiment 2

[0031] The method for preparing plant growth-promoting bacterium agent carrier matrix with green straw, comprises the steps:

[0032] (a) Raw material processing: Collect corn stalks with green leaves within 48 hours after harvesting ears. At this time, corn stalks with green leaves contain about 0.89% of total nitrogen, 0.41% of phosphorus, 0.22% of Mg, K1.59%, and Ca0.64 %, dry the corn stalks with leafy greens at 60°C, the moisture content in the corn stalks with green leaves is 7% after drying, cut the stalks with green leaves into sections and pulverize them with a pulverizer, and pass through a sieve with an aperture of 0.1mm ;

[0033] (b) Extraction of raw materials: leaching the sieved corn stalk powder with water at 80°C for 2 hours, wherein the weight ratio of straw powder to water is 1:50;

[0034] (c) Filtration and neutralization: filter after leaching, and adjust the pH of the filtrate to 7.0 with phosphate buffer;

[0035] (d) Drying: The filtrate was dried i...

Embodiment 3

[0037] The method for preparing plant growth-promoting bacterium agent carrier matrix with green straw, comprises the steps:

[0038] (a) Raw material processing: Collect corn stalks with green leaves within 48 hours after harvesting ears. At this time, corn stalks with green leaves contain about 0.89% of total nitrogen, 0.41% of phosphorus, 0.22% of Mg, K1.59%, and Ca0.64 %, dry the corn stalks with leafy greens at 70°C, after drying, the moisture content in the corn stalks with green leaves is 7%, cut the stalks with green leaves and use a pulverizer to crush them, and pass through a sieve with an aperture of 0.15mm ;

[0039](b) Extraction of raw materials: leaching the sieved corn stalk powder with water at 100°C for 1 hour, wherein the weight ratio of straw powder to water is 1:100;

[0040] (c) Filtration and neutralization: filter after leaching, and adjust the pH of the filtrate to 7.3 with phosphate buffer;

[0041] (d) Drying: Dry the filtrate at 60°C until the wat...

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PUM

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Abstract

The invention discloses a method for preparing a vector substrate of a plant growth promoting bacterium agent from green straws, which comprises the following steps of: (a) raw material processing; (b) raw material extracting; (c) filtration and neutralization; and (d) drying. In the method, the raw materials are sufficient and the price is low. Excessive soluble saccharides and proteins in the green straws are removed by adopting a hot water extracting or microwave irradiation extracting method, so that only a small amount of soluble carbon and nitrogen sources exists in green straw powder and more slightly soluble nutrient substances are decomposed and utilized by planted strains. The method has simple preparation process, good bacterium carrying effect and large viable bacteria quantity. The invention effectively solves the problems of the currently used bacterium agent vector. The method has high practicality and economy. The production and acquisition of the raw materials are environmental-friendly and non-polluted. The prepared bacterium agent product is convenient to use. The vector substrate can be degraded after being applied into the soil and the negative effects cannot be generated to the soil environment.

Description

technical field [0001] The invention relates to a method for preparing a carrier matrix of a plant growth-promoting bacterium agent, in particular to a method for preparing a carrier matrix of a plant growth-promoting bacterium agent with green straw. Background technique [0002] The plant growth-promoting agent refers to one or more probiotic strains or strains attached to the pores of a cheap and easy-to-use carrier material composed of organic or inorganic substances, or certain known molecules. The significance of the bacterial agent is to transfer the bacteria from the factory to the living plant rhizosphere. The effects of the application of bacterial agents on plant growth include nitrogen fixation in leguminous crops, biological control of soil-borne diseases, promotion of mineral absorption, weathering of soil minerals, nutritional or hormonal effects. Since the inoculated strain must compete with the indigenous microbial community already adapted to the local are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N25/08A01N63/02A01P21/00
Inventor 师尚礼李剑峰曹文侠霍平慧陈力玉张淑卿
Owner GANSU AGRI UNIV
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