A kind of wettable bacteria powder and its preparation method and application

A bacterial powder, wet technology, applied in the field of wettable bacterial powder and its preparation, can solve the problems of enhanced drug resistance, reduced control effect, low recovery rate, etc., to achieve good inhibitory effect, good wetting effect, high suspension rate Effect

Active Publication Date: 2022-04-22
CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tobacco red spot disease and wildfire disease are the main diseases that occur when tobacco leaves are mature, and the causes of the two are caused by different microorganisms, and the control agents are quite different. For wildfire disease, agricultural streptomycin is mainly used, and antibiotics are mainly used for prevention and control, so that the drug resistance is enhanced; as microbial agents are widely used in pesticides, microbial agents can be used to control tobacco leaves. Tobacco red spot disease and wildfire disease are treated. In the traditional technology, powder is the main method, and the powder spraying method is used to apply the drug, but the control target drug effect is poor, the dosage of the main agent per unit area is large, the recovery rate is low, and drift pollution Serious; in recent years, the research on wettable powder has become a new trend in the development of biological agent processing technology. Compared with powder, the effect is better, and the residual effect time is longer
[0004] However, the wettable bacterial powder in the prior art mainly realizes its wetting and other effects through the addition of additives, but its wetting effect is poor, and the number of viable bacteria in the bacterial powder is greatly reduced, which greatly reduces the control effect.

Method used

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  • A kind of wettable bacteria powder and its preparation method and application
  • A kind of wettable bacteria powder and its preparation method and application
  • A kind of wettable bacteria powder and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of concentrated fermented broth:

[0045] The strains of compound bacteria are cultured on multiple slopes to obtain fermented seeds, and inoculated into fermenters to obtain fermentation broth, wherein the culture temperature is 37°C, and the slant medium and fermentation broth are conventional bacterial culture components It is configured; the fermentation bacteria liquid is first centrifuged, the supernatant is removed, and the deposited mixture is filtered by plate and frame filter, and the pressures are 0.1MPa, 0.2MPa, 0.3MPa, 0.4MPa respectively. , 0.5MPa, 0.6MPa, record the time required for complete filtration of each group, in order to separate the bacteria remaining in the filter cake, after the filtration is completed, add water to the plate frame, which is called the top water amount, where the top water amount is The percentage composition of concentrated bacterial liquid total volume is 0%, 5%, 10%, 15%, 20%, detects the content of flora in the...

Embodiment 2

[0053] Preparation of bacterial agent mother powder:

[0054]The concentrated bacterial liquid obtained in Example 1 and the carrier material were mixed according to the mass ratio of 1:9, and spray-dried at 60°C to obtain the bacterial agent mother powder, wherein the carrier material was white carbon black (500 mesh) , talcum powder (800 mesh), kaolin (1250 mesh) and diatomaceous earth (800 mesh), measure the wettability of the bacterial preparation mother powder obtained by different carriers and the inhibitory effect on tobacco red spot disease and wildfire disease target bacteria: the The target bacteria suspension is added to the molten medium to make a mixed bacteria plate. After coagulation, 0.2 ml of antagonistic bacteria fermentation filtrate was added, and the diameter of the inhibition zone was measured by the cross method after incubation at 30° C. for 72 hours. Table 3 is the result of the wettability properties of the bacterial agent master powder, figure 1 , ...

Embodiment 3

[0059] Dispersant selection:

[0060] The bacterial agent master powder obtained in Example 2 with white carbon black as the carrier is mixed and dried with a dispersant, wherein the addition of the dispersant is 10%, wherein the dispersant is sodium tripolyphosphate, sodium lignosulfonate, carboxymethyl Sodium cellulose, PEG800, measure the wettability of the mother powder of bacterial preparation after adding the dispersant and the inhibitory effect on tobacco red spot and wildfire target bacteria (the specific method is the same as in Example 2), and table 4 is the wettable bacterial powder screening of dispersants, image 3 , 4 Microscopic examination results and inhibition zone of different dispersants are shown respectively.

[0061] Table 4 Screening of wettable powder dispersants

[0062]

[0063] It can be seen from Table 4 that the wetting time of sodium tripolyphosphate and PEG-800 is short, the suspension rate of sodium lignosulfamate and sodium tripolyphosph...

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Abstract

The invention relates to the technical field of microbial bacterial agents, in particular to a wettable bacterial powder and its preparation method and application. The mass percentages of the raw materials of the wettable bacterial powder are: 75%-90% of the mother powder of the bacterial agent, 5-10% of the dispersant, 5-10% of the wetting agent, 1-4% of the stabilizer, and 0.1% of the protective agent. ‑1.0%; the bacterial agent master powder is obtained by mixing and drying the concentrated bacterial liquid and the carrier. Through the screening of carriers, dispersants, wetting agents, stabilizers and protective agents and the setting of the ratio of each material, a wettable bacterial powder with good wetting effect, high suspension rate and high number of viable bacteria has been obtained, which can be widely used It is used for pesticide wettable bacterial powder.

Description

technical field [0001] The invention relates to the technical field of microbial bacterial agents, in particular to a wettable bacterial powder and its preparation method and application. Background technique [0002] Microbial agents are beneficial microorganisms isolated from nature. After screening and identification, they are processed into live bacterial preparations through fermentation, mixing, drying and other processes. They are widely used in the prior art. [0003] Tobacco red spot disease and wildfire disease are the main diseases that occur when tobacco leaves are mature, and the causes of the two are caused by different microorganisms, and the control agents are quite different. For wildfire disease, agricultural streptomycin is mainly used, and antibiotics are mainly used for prevention and control, so that the drug resistance is enhanced; as microbial agents are widely used in pesticides, microbial agents can be used to control tobacco leaves. Tobacco red sp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/22A01N63/20A01N25/14A01P3/00
CPCA01N63/22A01N63/20A01N25/14
Inventor 周志成刘天波尹华群孟德龙滕凯张明发曹明峰王振华周向平蔡海林肖艳松肖志鹏邹凯
Owner CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION
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