Straw micro-stage additive and preparation method thereof
An additive and straw technology, applied in the field of microorganisms, can solve problems such as traffic accidents affecting air quality, affecting crop growth, reducing soil fertility, etc., to achieve the effects of improving the internal and external environment of animals, solving the imbalance of flora in the body, and being widely used.
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[0046] 1. Materials:
[0047]SW-CJ-1CU ultra-clean workbench, domestic; YXQ-LS-50SI vertical pressure steam sterilizer, domestic; FD-1A-50 freeze dryer, domestic; BIOF-6050A50L mechanical stirring stainless steel intelligent fermentation tank , domestic; TGL cryogenic refrigerated centrifuge, domestic; L2800 microscope, domestic; UVD-3500 ultraviolet spectrophotometer, American Labomed; 3000B electronic balance, American; strains: Saccharomyces cerevisiae, Bacillus subtilis, lactic acid bacteria, Cytobacteria, nitrogen-fixing bacteria, and molds are preserved in the Pharmaceutical Laboratory of the School of Pharmacy and Bioengineering, Chongqing University of Technology.
[0048] 2. Method:
[0049] 2.1 Strain activation:
[0050] Put the preserved brewer's yeast, lactic acid bacteria, Bacillus subtilis, actinomycetes, nitrogen-fixing bacteria, and mold strains into sterilized 1% sugar water to activate for 2 hours, and then transfer them into sterilized bean sprouts juice,...
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