Culture medium for effectively prolonging survival time of lactic acid bacteria and preparation method of culture medium

A technology of lactic acid bacteria and survival period, applied in the direction of bacteria, etc., can solve problems such as unsatisfactory effects, and achieve the effects of increasing carbon source intake channels, enhancing vitality, and reducing costs

Active Publication Date: 2017-02-22
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the effect of the existing substratum that is used to prolong the survival period of lactic acid bacteria live bacteria is not ideal, and it is far from satisfying people's needs

Method used

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  • Culture medium for effectively prolonging survival time of lactic acid bacteria and preparation method of culture medium
  • Culture medium for effectively prolonging survival time of lactic acid bacteria and preparation method of culture medium
  • Culture medium for effectively prolonging survival time of lactic acid bacteria and preparation method of culture medium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: The culture medium that effectively prolongs the survival period of lactic acid bacteria is prepared from the following raw materials in parts by weight (30 g per part): 0.08 part of peptone, 0.08 part of yeast extract powder, 1.5 part of manno-oligosaccharide, skimmed milk powder 0.8 parts, 0.5 parts of defatted soybean meal, 0.05 parts of magnesium sulfate heptahydrate, 0.025 parts of manganese sulfate heptahydrate, 1.5 parts of corn steep liquor dry powder, 0.008 parts of compound additives, 0.025 parts of sodium dihydrogen phosphate, 0.25 parts of sodium citrate, 0.2 parts of citric acid , 0.08 part of sodium acetate, 0.08 part of Tween 80, 0.04 part of saccharomyces cerevisiae; the composite additive is a mixture of sodium carboxymethyl cellulose, sodium alginate, bone powder, royal jelly acid, guar gum and xanthan gum, And the mass ratio is sodium carboxymethyl cellulose: sodium alginate: bone meal: royal jelly acid: guar gum: xanthan gum=2:2:3:1:1:2;...

Embodiment 2

[0047] Embodiment 2: The substratum that effectively prolongs the survival period of lactic acid bacteria, its active ingredient is made of the raw materials of following weight (every part gets 15g): 0.12 parts of peptone, 0.12 parts of yeast extract powder, 2.5 parts of mannan oligosaccharides, 1.2 parts of skimmed milk powder 0.7 parts of defatted soybean meal, 0.07 parts of magnesium sulfate heptahydrate, 0.035 parts of manganese sulfate heptahydrate, 2.5 parts of corn steep liquor dry powder, 0.012 parts of compound additives, 0.035 parts of sodium dihydrogen phosphate, 0.35 parts of sodium citrate, 0.3 parts of citric acid, 0.12 parts of sodium acetate, 0.12 parts of Tween 80, and 0.06 parts of Saccharomyces cerevisiae; the composite additive is a mixture of sodium carboxymethyl cellulose, sodium alginate, bone meal, royal jelly acid, guar gum and xanthan gum, and The mass ratio is sodium carboxymethyl cellulose: sodium alginate: bone meal: royal jelly acid: guar gum: xan...

Embodiment 3

[0055] Embodiment 3: the substratum that effectively prolongs the survival period of lactic acid bacteria, its active ingredient is made of the raw materials of following weight (every part gets 25kg): 0.09 part of peptone, 0.09 part of yeast extract powder, 1.8 part of mannan oligosaccharide, 0.9 part of skimmed milk powder 0.55 parts of defatted soybean meal, 0.055 parts of magnesium sulfate heptahydrate, 0.028 parts of manganese sulfate heptahydrate, 1.8 parts of corn steep liquor dry powder, 0.009 parts of compound additives, 0.028 parts of sodium dihydrogen phosphate, 0.28 parts of sodium citrate, 0.23 parts of citric acid, 0.09 part of sodium acetate, 0.09 part of Tween 80, 0.045 part of Saccharomyces cerevisiae; the compound additive is a mixture of sodium carboxymethyl cellulose, sodium alginate, bone meal, royal jelly acid, guar gum and xanthan gum, and The mass ratio is sodium carboxymethyl cellulose: sodium alginate: bone meal: royal jelly acid: guar gum: xanthan gum...

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Abstract

The invention discloses a culture medium for effectively prolonging the survival time of lactic acid bacteria and a preparation method of the culture medium. The culture medium for effectively prolonging the survival time of the lactic acid bacteria is prepared by taking peptone, yeast extract powder, mannan oligosaccharide, skim milk powder, defatted soybean meal, magnesium sulfate heptahydrate, manganese sulfate heptahydrate, dried corn steep liquor powder, a compound additive, sodium dihydrogen phosphate, sodium citrate, citric acid, sodium acetate, Tween-80 and saccharomyces cerevisiae as raw materials; in addition, the compound additive is a mixture of sodium carboxymethylcellulose, sodium alginate, bone meal, royal jelly acid, guar gum and xanthan gum. According to the culture medium for effectively prolonging the survival time of the lactic acid bacteria, the functions of all the raw materials are mutually cooperative, and higher variable bacteria quantity of the lactic acid bacteria can be kept at normal temperature for a long time; in addition, the culture medium has the advantages of simplicity and convenience in operation, easily-obtained raw materials, low cost and high homogenization degree of a product; the lactic acid bacteria is enabled to be more stable in property and higher in biological activity and can be stored for a longer time.

Description

technical field [0001] The invention relates to a medium for effectively prolonging the survival period of lactic acid bacteria and a preparation method thereof. Background technique [0002] Since the Russian biologist Mechnikov discovered the important role of lactic acid bacteria, the application of lactic acid bacteria and lactic acid bacteria beverages in various countries in the world has been quite popular, and people's understanding of it is getting deeper and deeper. A large number of animal and clinical trials at home and abroad have proved that lactic acid bacteria have good health care and therapeutic effects on humans and animals. Its main functions are: to improve the function of the gastrointestinal tract and maintain the balance of intestinal flora. Lactic acid bacteria are common bacteria in the intestinal tract, which can change the intestinal environment of animals, inhibit the reproduction of harmful bacteria, and adjust the balance of gastrointestinal fl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 杨晓慧
Owner QILU UNIV OF TECH
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