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A kind of lactic acid bacteria preservative that improves the survival rate of liquid lactic acid bacteria at normal temperature and its application

A technology of lactic acid bacteria and preservatives, applied in the direction of preservation of microorganisms, methods based on microorganisms, microorganisms, etc., can solve the problem of the decline of viable bacteria, achieve long-term storage, delay the time of decay, and improve the survival rate

Active Publication Date: 2020-08-11
青岛云溯源健康科技有限公司
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem that the amount of viable bacteria in liquid lactic acid bacteria is seriously reduced after long-term storage at normal temperature, the present invention provides a lactic acid bacteria preservative and its application that can improve the survival rate of liquid lactic acid bacteria at normal temperature. The lactic acid bacteria protective agent uses cream and The emulsifier wraps the nutritional complex of lactic acid bacteria, improves the suspension and stability of lactic acid bacteria and nutrients at the same time, and significantly reduces the loss rate of live bacteria after long-term storage of liquid lactic acid bacteria

Method used

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  • A kind of lactic acid bacteria preservative that improves the survival rate of liquid lactic acid bacteria at normal temperature and its application

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Effect test

Embodiment 1

[0030] The liquid medium of this embodiment is used as a control group, and its liquid medium formula is taken by weight: 0.1 part of peptone; 0.1 part of yeast extract powder; 5 parts of white granulated sugar; 2 parts of glucose; 1 part of skimmed milk powder; part; 0.03 part of manganese sulfate heptahydrate; 2 parts of corn steep liquor dry powder; NaH 2 PO 4 0.03 parts; 0.3 parts of sodium citrate; 0.25 parts of citric acid; 0.1 parts of sodium acetate; 0.1 parts of Tween 80; add 86 parts of purified water. 115°C, autoclave for 20 minutes.

[0031] Choose 3 kinds of lactic acid bacteria, namely: Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus casei ( Lactobacillus casei ), Lactobacillus plantarum ( lactobacillus plantarum ). Add 3 parts of mixed lactic acid bacteria seed solution to the above liquid medium, culture at 37°C for 36 hours, and aseptically dispense. At the same time, the number of lactic acid bacteria colonies was measured as ...

Embodiment 2

[0036] The formula of the liquid medium in this embodiment is weighed in parts by weight: 0.1 part of peptone; 0.1 part of yeast extract powder; 5 parts of white sugar; 2 parts of glucose; 1 part of milk powder; 4 parts of cream; 1 part of linoleic acid; resistant dextrin 0.05 parts; 0.06 parts of magnesium sulfate heptahydrate; 0.03 parts of manganese sulfate heptahydrate; 2 parts of corn steep liquor dry powder; sodium carboxymethyl cellulose: sodium alginate: guar gum: xanthan gum=2:2:1:2 0.2 parts; NaH2 PO 4 0.03 parts; 0.3 parts of sodium citrate; 0.25 parts of citric acid; 0.1 parts of sodium acetate; 0.1 parts of lecithin; 115°C, autoclave for 20 minutes. Adjust the pH value of the culture solution to 3.5 to avoid the sedimentation point of most of the peptone and protein, so that the nutrients can be fully utilized.

[0037] Sodium carboxymethyl cellulose: sodium alginate: guar gum: xanthan gum is mixed according to the ratio of 2:2:1:2 to form 0.2 parts by weight; ...

Embodiment 3

[0051] The liquid lactic acid bacteria that embodiment 1 and embodiment 2 obtain (3 * 10 9 cfu / ml) for animal experiments to observe the effects of two liquid lactic acid bacteria on the growth performance of white-feathered broilers. Both liquid lactic acid bacteria were placed at room temperature of 25°C, and white feather broiler chickens were given drinking water from the age of 4 days, and the addition amount was 1000ml / ton of water. Finally, the difference in the growth performance of white feather broiler chickens between the lactic acid bacteria cultured in the two media was observed.

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Abstract

The invention provides a lactobacillus preservative capable of increasing the survival rate of liquid lactobacilli at the room temperature and an application of the lactobacillus preservative. With adoption of a lactobacillus body and nutrient suspension technology and a sustained release technology as well as addition of cream, linoleic acid, lecithin, glycerol monostearate and a stabilizer to a medium and regulation of pH value, the storage period of the liquid lactobacilli with high live bacteria quantity is prolonged, the problem of rapid reduction of liquid lactobacilli with high live bacteria quantity due to reduction of the content of nutrients and storage at the normal temperature is solved, and the lactobacilli really play a role in preventing and treating diarrhea and regulating balance of the intestinal flora during use; the lactobacillus preservative as a green, safe and effective microecological agent is applied and popularized.

Description

technical field [0001] The invention belongs to the field of animal health food, and in particular relates to a lactic acid bacteria preservative for improving the survival rate of liquid lactic acid bacteria at normal temperature and an application thereof. Background technique [0002] Since the Russian biologist Mechnikov discovered the important role of lactic acid bacteria, the application of lactic acid bacteria and lactic acid bacteria beverages in various countries in the world has been quite popular, and people's understanding of it is getting deeper and deeper. As a feed additive, lactic acid bacteria play an important role in maintaining the balance of animal intestinal flora, improving immunity, reducing feed-to-meat ratio, and improving animal production performance. [0003] Lactic acid bacteria have health care and therapeutic effects on humans and animals, which have been proved by a large number of animal and clinical trials at home and abroad. Its main fun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12R1/23C12R1/245C12R1/25C12R1/46C12R1/01
CPCC12N1/04
Inventor 王希辉张庆杰郭文龙桑艳玲
Owner 青岛云溯源健康科技有限公司
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