Trichoderma harzianum and application thereof

A technology of Trichoderma trichoderma and Trichoderma, applied in the field of microorganisms, can solve the problems of poor control effect of tomato damping disease, inconvenient application, dust pollution, etc., and achieves easy continuous production, not easy to agglomerate, and no dust pollution. Effect

Active Publication Date: 2021-06-01
SHANGHAI WANLIHUA BIOTECH
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing microbial preparations have poor control effects on tomato damping-off, or have the disadvantages of easy caking during storage, dust pollution during application, and inconvenient application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Trichoderma harzianum and application thereof
  • Trichoderma harzianum and application thereof
  • Trichoderma harzianum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This embodiment provides a kind of Trichoderma strigosum, which is collected and separated from the soil of Sanqi rhizosphere in Wenshan City, Yunnan Province. The Latin scientific name of the above-mentioned Trichoderma strigosum is Trichoderma strigosum, the strain number is TR2125, and the preservation number is CGMCCNo. 21430.

[0042] The Trichoderma strigosum strain of the present invention is preserved in the General Microorganism Center (CGMCC) of the China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is January 2021 13th.

[0043] Taxonomy and identification of Trichoderma hardifolia:

[0044] The growth rate of the above-mentioned Trichoderma duodenum TR2125 colony is moderately fast, and the diameter of the colony is 4-6 cm after three days of growth; most of the hyphae are endogenous, and the aerial mycelium with limited growth on t...

Embodiment 2

[0056] The present embodiment provides a kind of Trichoderma clementum fermented liquid, the preparation method of above-mentioned Trichoderma clematis fermented liquid, comprises the steps:

[0057] Step 1, the Trichoderma ciliate species that embodiment 1 isolates is inoculated in the PDA medium, and is cultivated at constant temperature;

[0058] Step 2, transfer the strains cultivated in step 1 to the culture medium and carry out shaking flask culture for 24 hours to obtain the first-grade seed liquid;

[0059] Step 3: After sterilizing the culture solution in the intermediate fermenter at 121°C and 15 lbs pressure for 20 minutes, keep the positive pressure, cool to 30°C, then pour the primary seed liquid obtained in step 2 into the intermediate fermenter, add Pressurize sterile air, and ferment for 6 days at 28-30°C, 0.4-0.5kg pressure, 1500-1800L / H air flow rate and 120-150rpm speed bar, to obtain Trichoderma ilicum fermentation liquid;

[0060] In step 2 and step 3, ab...

Embodiment 3

[0064] The present embodiment provides a kind of microbial inoculant, contains the Trichoderma ciliate fermented liquid of above-mentioned embodiment 2 gained, comprises the component of following percentage by weight:

[0065]

[0066] The above-mentioned microbial inoculum is prepared by the following method: after mixing evenly the carrier, mineral potassium humate, peat, wetting agent, dispersant, disintegrating agent, binder and moisturizing agent according to the proportion, spray the fermented liquid of Trichoderma hardii , and stir evenly until the water content of the mixture is 40-45%; after extrusion and granulation by the granulator, the dehumidifier dehumidifies it for 3 hours until the water content of the granules is 8-10%, and the finished product is the above-mentioned microbial bacterial agent.

[0067] The detected spore content was 3×10 8 CFU / g. According to the GB20287-2006 testing standard for agricultural microbial agents, the indicators such as pH, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses trichoderma harzianum and an an application thereof, the Latin name is Trichoderma harzianum, the strain number of the trichoderma harzianum is TR2125, and the preservation number of the trichoderma harzianum is CGMCC No.21430. The fermentation liquor obtained by fermenting the trichoderma hirsutum is used for preparing a microbial agent, and the obtained microbial agent has a good prevention and treatment effect on tomato damping-off; the fermentation liquor is not easy to cake during storage, is free of dust pollution during application and is convenient to apply; and the product has the advantages of good dispersion performance and high viable count, the spore content reaches 3*10 <8> CFU / g, and the chlamydospore content is still more than 10<8> CFU / g after the product is stored for 12 months.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a kind of Trichoderma hardii and its application. Background technique [0002] Trichoderma (including "hard hair" Trichoderma) is a microorganism that can inhibit and kill a variety of plant pathogenic bacteria in nature. Human beings make use of the excellent characteristics of Trichoderma to make different dosage forms of bio-pesticides and bio-fertilizers through specific processing technology. Trichoderma is widely distributed, easy to isolate and cultivate, can produce chlamydospores with strong stress resistance, has the characteristics of easy storage and convenient use, and has the following effects: preventing and controlling various plant diseases and promoting plant growth. [0003] Tomato is an important economic crop in my country. Tomato damping-off is an important disease caused by Pythium ultimum Trow of the flagellate subphylum. Typical symptoms are pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N11/14C12R1/885
CPCC12N1/14C12N11/14
Inventor 于稳欠糜芳陈晨毛伟力
Owner SHANGHAI WANLIHUA BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap