Trichoderma harzianum and application thereof
A technology of Trichoderma trichoderma and Trichoderma, applied in the field of microorganisms, can solve the problems of poor control effect of tomato damping disease, inconvenient application, dust pollution, etc., and achieves easy continuous production, not easy to agglomerate, and no dust pollution. Effect
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Embodiment 1
[0041] This embodiment provides a kind of Trichoderma strigosum, which is collected and separated from the soil of Sanqi rhizosphere in Wenshan City, Yunnan Province. The Latin scientific name of the above-mentioned Trichoderma strigosum is Trichoderma strigosum, the strain number is TR2125, and the preservation number is CGMCCNo. 21430.
[0042] The Trichoderma strigosum strain of the present invention is preserved in the General Microorganism Center (CGMCC) of the China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is January 2021 13th.
[0043] Taxonomy and identification of Trichoderma hardifolia:
[0044] The growth rate of the above-mentioned Trichoderma duodenum TR2125 colony is moderately fast, and the diameter of the colony is 4-6 cm after three days of growth; most of the hyphae are endogenous, and the aerial mycelium with limited growth on t...
Embodiment 2
[0056] The present embodiment provides a kind of Trichoderma clementum fermented liquid, the preparation method of above-mentioned Trichoderma clematis fermented liquid, comprises the steps:
[0057] Step 1, the Trichoderma ciliate species that embodiment 1 isolates is inoculated in the PDA medium, and is cultivated at constant temperature;
[0058] Step 2, transfer the strains cultivated in step 1 to the culture medium and carry out shaking flask culture for 24 hours to obtain the first-grade seed liquid;
[0059] Step 3: After sterilizing the culture solution in the intermediate fermenter at 121°C and 15 lbs pressure for 20 minutes, keep the positive pressure, cool to 30°C, then pour the primary seed liquid obtained in step 2 into the intermediate fermenter, add Pressurize sterile air, and ferment for 6 days at 28-30°C, 0.4-0.5kg pressure, 1500-1800L / H air flow rate and 120-150rpm speed bar, to obtain Trichoderma ilicum fermentation liquid;
[0060] In step 2 and step 3, ab...
Embodiment 3
[0064] The present embodiment provides a kind of microbial inoculant, contains the Trichoderma ciliate fermented liquid of above-mentioned embodiment 2 gained, comprises the component of following percentage by weight:
[0065]
[0066] The above-mentioned microbial inoculum is prepared by the following method: after mixing evenly the carrier, mineral potassium humate, peat, wetting agent, dispersant, disintegrating agent, binder and moisturizing agent according to the proportion, spray the fermented liquid of Trichoderma hardii , and stir evenly until the water content of the mixture is 40-45%; after extrusion and granulation by the granulator, the dehumidifier dehumidifies it for 3 hours until the water content of the granules is 8-10%, and the finished product is the above-mentioned microbial bacterial agent.
[0067] The detected spore content was 3×10 8 CFU / g. According to the GB20287-2006 testing standard for agricultural microbial agents, the indicators such as pH, ...
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