A kind of astragalus probiotics and its preparation method and application

The technology of a microecological preparation and astragalus is applied in the application, animal feed, additional food elements, etc., and can solve the problems of high cost of fermentation medium, the increase rate of crude polysaccharide needs to be further improved, and the increase of inorganic phosphorus pollution in livestock and poultry manure, etc. Achieve the effect of increasing the content of crude polysaccharide and lactic acid bacteria, increasing the content of crude polysaccharide and lactic acid bacteria in Astragalus, and improving animal production performance

Active Publication Date: 2018-01-12
河南天昊鸿发生物技术有限公司
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zhu Xinshu disclosed in "Lactobacillus Breeding of Fermented Astragalus and Optimization of Fermentation Parameters" that the water extract of Astragalus fermented by Lactobacillus can improve the yield of Astragalus polysaccharides and the number of cells, and optimized the fermentation medium as corn flour 42.10 g / L, nitrogen source 7.45g / L, carrot powder 0.80g / L, KH 2 PO 4 -K 2 HPO 4 2.18g / L, meat extract 6.25g / L, the initial pH of the fermentation medium is 6.7, the fermentation time is 68h, and the fermentation temperature is 38°C. The fermentation medium uses lactic acid bacteria to ferment Astragalus membranaceus. Although the yield of crude polysaccharides has been increased, the increase rate of crude polysaccharides needs to be further improved, and the cost of the fermentation medium is high, and inorganic phosphorus is added, which increases the inorganic phosphorus pollution of livestock and poultry manure. The problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of astragalus probiotics and its preparation method and application
  • A kind of astragalus probiotics and its preparation method and application
  • A kind of astragalus probiotics and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:

[0030] 1) Raw material pretreatment:

[0031] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;

[0032] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust pH to 6.8, and autoclave to prepare MRS medium, Enterococcus faecium and Lactobacillus plantarum into MRS medium at a ratio of 5:5, anaerobic culture at 37°C 24h, that is;

[0033] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;

[0034] 2) Fermented astragalus: After mixin...

Embodiment 2

[0037] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:

[0038] 1) Raw material pretreatment:

[0039] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;

[0040] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust the pH to 6.8, and autoclave to prepare the MRS medium, enter the Enterococcus faecium and Lactobacillus plantarum into the MRS medium at a ratio of 5:5, and culture anaerobically at 38°C 30h, that is;

[0041] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;

[0042]2) Ferm...

Embodiment 3

[0045] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:

[0046] 1) Raw material pretreatment:

[0047] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;

[0048] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust the pH to 6.8, and autoclave to prepare MRS medium. Enter the Enterococcus faecium and Lactobacillus plantarum into the MRS medium at a ratio of 5:5, and cultivate anaerobically at 39°C 36h, that is;

[0049] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;

[0050] 2) Ferme...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an astragalus microecological preparation, a preparation method and application thereof, and belongs to the technical field of feed additives and biological fermentation traditional Chinese medicines. The micro-ecological preparation is mainly fermented from Astragalus membranaceus, lactic acid bacteria seed liquid and solid medium; the lactic acid bacteria seed liquid is prepared by mixed culture of Enterococcus faecium and Lactobacillus plantarum at a ratio of 2-8:8-2; every 25-75 The number of living bacteria in the lactic acid bacteria seed liquid corresponding to the inoculation of gram Astragalus membranaceus is 25 × 109. After fermentation, compared with the traditional additive that directly mixes lactic acid bacteria and Astragalus membranaceus, the Astragalus membranaceus microecological preparation of the present invention improves the yield of crude polysaccharides and lactic acid bacteria quantity. The preparation method of the astragalus microecological preparation of the invention improves the synergistic effect of astragalus and lactic acid bacteria by selecting a suitable fermentation temperature and fermentation time. The astragalus probiotics of the invention is used as a feed additive for livestock and poultry.

Description

technical field [0001] The invention belongs to the technical field of feed additives and bio-fermentation traditional Chinese medicines, and specifically relates to a microecological preparation, a preparation method and an application. Background technique [0002] With the abuse of antibiotics, hormones, etc. Additives will have more and more important applications in animal husbandry production. Microecological preparations are probiotics, prebiotics, and combinations of probiotics and prebiotics. The important sources of probiotics and prebiotics are lactic acid bacteria and plant polysaccharides respectively. However, traditional microecological preparations simply combine lactic acid bacteria and plant polysaccharides. The huge potential of microbial fermentation has not been brought into play. Studies have shown that microbial fermentation of traditional Chinese medicine has outstanding advantages such as increasing the content of active ingredients in traditional C...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/18A23K20/00
Inventor 乔宏兴边传周王永芬史洪涛赵圣振李华玮姜亚乐张菡
Owner 河南天昊鸿发生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap