Method for extracting heterotrophic chlorella neutral oil

A technology of heterotrophic chlorella and chlorella, applied in the direction of fat oil/fat production, fat oil/fat refining, fat production, etc., can solve the problem of no wet algae oil extraction method, etc., and achieve the effect of efficient extraction

Inactive Publication Date: 2012-07-11
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the field of microalgae energy, there is no wet algae oil extraction method that can be used for industrial production

Method used

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  • Method for extracting heterotrophic chlorella neutral oil
  • Method for extracting heterotrophic chlorella neutral oil
  • Method for extracting heterotrophic chlorella neutral oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Heterotrophic chlorella was selected from C.protothecoides 0710 ("A method for producing biodiesel through high-density fermentation of heterotrophic chlorella CN101230364A" applied by Microalgae Bioenergy Laboratory, School of Life Sciences, Tsinghua University), through high-density heterotrophic Fermentation technology makes it rich in a large amount of neutral oils. Algae cell density higher than 50gl -1 The fermentation broth was centrifuged at 6000g for 4 minutes to fully precipitate the heterotrophic chlorella cells, removed the supernatant, added a small amount of water, oscillated to resuspend the cells, and then centrifuged at 6000g for 4 minutes to make the heterotrophic chlorella cells Fully precipitate, remove the supernatant, and repeat the above operation again to achieve the purpose of fully removing the culture medium and washing the algae cells. Take 30.00 g of wet algae and freeze-dry at -50° C. for 48 hours until constant weight to obtain 10.23 g of...

Embodiment 2

[0025] Freeze-dry the wet algae at -50° C. for 48 hours to constant weight to obtain dry algae powder (water content < 1 wt%). Ball milling of dry algae powder on a planetary ball mill: mix 30g of dry algae powder with 30ml of grinding solvent (ethanol, isopropanol or n-hexane), and then add 200g and 400g (diameter 5mm-10mm) of zirconia mill respectively The medium balls were put into a ball mill jar with a volume of 500ml, and after proper mixing, they were installed on a planetary ball mill for comparative experiments, and ball milled at 100rpm for 0.5, 1, 2, 4, and 6 hours respectively. Add 300ml of extraction solvent (chloroform, isopropanol or n-hexane) to the ball mill jar, filter and separate the mill balls with a filter screen with a pore size of 2mm, and obtain the algae debris / solvent mixture. Centrifuge the algae debris / grinding solvent mixture at 6000g for 4 minutes, retain the upper liquid, and distill under reduced pressure at 65°C until the solvent is completely...

Embodiment 3

[0027] For wet algae without drying treatment, ball milling optimization is as follows: 30g of wet algae (water content is 61.9 ± 3.1wt%) is mixed with 30ml of grinding solvent (ethanol, isopropanol or n-hexane), and then 200g, 400g (Diameter 5mm-10mm) into a ball milling jar with a volume of 500ml, after mixing properly, install it on a planetary ball mill for comparative experiments, and ball mill at 100rpm for 6, 12, and 24 hours respectively. Add 300ml of extraction solvent (chloroform, isopropanol or n-hexane) to the ball mill jar, filter and separate the mill balls with a filter screen with a pore size of 2mm, and obtain the algae debris / solvent mixture. The mixture was subjected to ultrasonic crushing under the following conditions: power 600W, frequency 20KHz, working time 3 seconds, interval time 3 seconds, total time 40 minutes. Subsequently, the algae debris / grinding solvent mixture was centrifuged at 6000 g for 4 minutes, the upper solvent was retained, and the sol...

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Abstract

The invention belongs to the field of renewable energy, and discloses a method for extracting heterotrophic chlorella neutral oil. According to the invention, a large amount of heterotrophic chlorella cultivated by fermentation in a bioreactor is adopted as a raw material. With mechanism crushing of a ball mill, with an auxiliary mass transfer effect of ultrasonic waves, and with the soakage of a low-pole organic solvent, cell walls of dried chlorella and cell walls of chlorella that is not dried or dehydrated are crushed; the material is extracted by using a neutral solvent, such that crude chlorella neutral oil is obtained. For completely dried chlorella cells, the extraction rate of neutral oil reaches 99.27%. For water-containing chlorella cells, the extraction rate of neutral oil reaches 89.61%. With the method, the obtained neutral oil can be directly used for preparing biodiesel, and the chlorella neutral oil extraction time is shortened. Therefore, the method has industrial production potential. With the method, a large amount of raw material oil is provided for large-scale productions of biodiesel. The method is a rapid and simple chlorella neutral oil extraction approach.

Description

technical field [0001] The invention belongs to the field of renewable energy, in particular to a method for extracting neutral oil from heterotrophic chlorella. Specifically, the chlorella that has not been dried and dehydrated is mechanically crushed by a ball mill and assisted by ultrasonic mass transfer, and in the infiltration of a weakly polar organic solvent, the cell wall of the chlorella is broken, and the oil component is extracted. The neutral oil obtained through extraction and refining with a neutral organic solvent can be used to prepare biodiesel through transesterification. Background technique [0002] Facing the gradual depletion of fossil fuels worldwide, the search for a new generation of renewable energy is becoming the focus of the world's attention. Among them, microalgae biodiesel is regarded as the most competitive renewable liquid biofuel that can completely replace fossil fuels without affecting the production of food and other agricultural produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04C11B3/00
CPCY02E50/13Y02E50/10
Inventor 吴庆余戴俊彪卢悦刘敏盛
Owner TSINGHUA UNIV
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