Method for promoting accumulation of oil in microalgae and keeping high biomass of microalgae

A technology of microalgae oil and high biomass, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of adding plant hormones, etc., achieve simple operation, good applicability, and increase the biomass of microalgae effect of concentration

Active Publication Date: 2017-11-24
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has been no report on the addition of phytohormones under nitrogen-deficient culture conditions so far.

Method used

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  • Method for promoting accumulation of oil in microalgae and keeping high biomass of microalgae
  • Method for promoting accumulation of oil in microalgae and keeping high biomass of microalgae
  • Method for promoting accumulation of oil in microalgae and keeping high biomass of microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Growth characteristics of Chlorella SDEC-6

[0053] 1. Algae culture process:

[0054] (1) Put Chlorella SDEC-6 in BG11 medium for enrichment culture, the composition of BG11 medium is as follows: NaNO 3 1.5g / L, K 2 HPO 4 40mg / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 2H 2 O 36mg / L, citric acid 6 mg / L, ferric ammonium citrate 6mg / L, EDTANa 2 1mg / L,Na 2 CO 320mg / L, A5 solution 1mL / L; A5 solution is: H 3 BO 3 2.86g / L, MnCl 2 4H 2 O 1.86g / L, ZnSO 4 ·7H 2 O 0.22g / L,Na 2 MoO 4 2H 2 O 0.39 g / L, CuSO 4 ·5H 2 O0.08g / L, Co(NO 3 ) 2 ·6H 2 O 0.05g / L.

[0055] (2) The algal seed liquid obtained in step (1) is centrifuged, washed three times with nitrogen-deficient medium, and then reinoculated in nitrogen-deficient medium as a control group, with an initial dry weight of 0.11g / L.

[0056] (3) After centrifuging the algae seed liquid obtained in step (1), washing the nitrogen-deficient medium three times, re-inoculating in the nitrogen-deficient BG11 medium...

Embodiment 2

[0064] Example 2: Oil Accumulation Properties of Chlorella SDEC-6

[0065] 1. Determination of oil content:

[0066] Weigh about 0.1 g of the dried algae powder obtained in Example 1 into a 50 mL centrifuge tube, add 10 mL of chloroform / methanol (v / v=2:1) ​​solution, and process it with an ultrasonic breaker for 10 min (frequency 20%), then 4000 r After centrifugation, the solution was separated into two phases, and the supernatant was transferred to a 60mL separatory funnel, and the whole extraction process was repeated twice. According to the volume of the oil extract, add 0.9% sodium chloride solution (adding volume is 1 / 5 of the oil extract, about 4-5mL sodium chloride solution), shake well for 1 min, and let stand for 15 min. Measure the volume of the low-phase solution, and take 5mL of the low-phase solution in a 10mL glass test tube, blow dry with nitrogen, and place the glass tube in a 60°C oven to dry to constant weight (about 30min).

[0067] 2. Calculation of fat ...

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Abstract

The invention discloses a method for promoting the accumulation of oil in microalgae and keeping the high biomass of the microalgae. The method is concretely characterized in that enrichment-cultured microalgae are cultured in a nitrogen-deficient medium containing a plant hormone. The plant hormone indoleacetic acid is added to the nitrogen-deficient medium for the first time in order to stimulate the accumulation of oil in microalgae and keep the high biomass of the microalgae, so the microalgae can still keep a high biomass under a nitrogen deficiency condition. Test proves that the method makes the oil content of chlorella higher than the oil content of the chlorella cultured in a nitrogen-free medium free from the plant hormone, so the plant hormone can be used as a good means for stimulating the microalgae to produce oil. The method has the advantages of simplicity in operation, short time, high efficiency, easiness in realization, effective increase of the biomass concentration of the microalgae under a nitrogen-deficient culture condition, and good applicability, and provides an effective technical scheme for microalgae to produce biodiesel or develop other biomass energy sources.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a method for adding plant hormones to a nitrogen-deficient medium to rapidly promote the accumulation of microalgae oil while maintaining the biomass of microalgae. Background technique [0002] As a new resource for producing animal feed, food additives, cosmetics and health care products, microalgae has attracted extensive attention from researchers all over the world. Not only that, because microalgae has the characteristics of short growth cycle, small footprint and strong environmental adaptability, researchers have used it as a third-generation high-quality raw material for biodiesel production. However, the oil content and oil yield of some microalgae are not satisfactory, which limits their large-scale commercial application. Therefore, researchers have tried various methods and means to stimulate oil accumulation in microalgae. [0003] The researchers found that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38C12R1/89
CPCC12N1/12C12N1/38
Inventor 裴海燕于泽蒋丽群
Owner SHANDONG UNIV
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