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Culture media composition suitable for cultivating high-density high-quality ordinary chlorella

A technology of common chlorella and culture medium, which is applied in the field of high-density and high-quality culture of common chlorella, and can solve problems such as inability to achieve high-density and high-quality culture.

Active Publication Date: 2006-07-26
JIAXING ZEYUAN BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This shows that the high-density and high-quality cultivation of the algae cannot be achieved by using the Endo medium and the SK medium with glucose added. Therefore, it is necessary to solve the medium problem of the high-density and high-quality cultivation of Chlorella vulgaris

Method used

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  • Culture media composition suitable for cultivating high-density high-quality ordinary chlorella
  • Culture media composition suitable for cultivating high-density high-quality ordinary chlorella
  • Culture media composition suitable for cultivating high-density high-quality ordinary chlorella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Add 100ml of the medium shown below to a 250ml shake flask, and the inoculation concentration is 0.62g / L. Heterotrophic culture (100mL liquid volume, 30°C temperature, 150r / min shaker speed) for 48 hours, the result is to measure the cell density () was 13.17g / L at the maximum, followed by photoautotrophic culture (external light intensity 8klx), at the end of the culture (96h) the contents of algae protein () and chlorophyll () reached the highest, 49.75% and 30.17mg / gDcw respectively (See figure 1 ).

[0039] KNO 3 : 9.25 Glucose: 30

[0040] KH 2 PO 4 : 0.7 Na 2 HPO 4 ·12H 2 O: 1.84

[0041] MgSO 4 ·7H 2 O: 0.7CaCl 2 : 0.142

[0042] FeSO 4 ·7H 2 O: 0.02

[0043] Trace element: 1ml; trace element formula (g / L): H 3 BO 3 11.42 ZnSO 4 ·7H 2 O 8.82

[0044] MnCl 2 ·H 2 O 1.42 (NH 4 ) 6 Mo 7 O 24 ·4H 2 O 0.8707

[0045] CuSO 4 ·5H 2 O 1.57 Co(NO 3 ) 2 ·6H 2 O 0.49

[004...

Embodiment 2

[0064] In a 250ml shake flask add 100ml of the medium shown below with KNO 3 The initial concentrations were 3, 5, 7, 9, and 11 g / L respectively, the inoculation concentration was 0.60 g / L, and the culture was carried out for 82 hours (wherein the heterotrophic culture was 45 hours, wherein the liquid filling volume was 100 mL, the temperature was 30 ° C, and the shaker speed was 150 r / min; photoautotrophic culture for 37 hours, external light intensity 8klx). The result measured KNO 3 At 7~9g / L (calculated as 10.20~13.12 by C / N ratio), the maximum cell density is about 12.0g / L (see image 3 ), the protein and chlorophyll content of the algae also reached the highest levels, around 50.0% and 32.0 mg / gDcw, respectively (see Figure 4 ).

[0065] KNO 3 : 3, 5, 7, 9, 11 Glucose: 30

[0066] KH 2 PO 4 : 0.7 Na 2 HPO 4 ·12H 2 O: 1.84

[0067] MgSO 4 ·7H 2 O: 0.7CaCl 2 : 0.142

[0068] FeSO 4 ·7H 2 O: 0.02

[0069] Trace elements: 1ml

[0070] Trace element formu...

Embodiment 3

[0075] The medium shown below was added to the 5L bioreactor / 1L open plate photobioreactor series system, the filling volume was 75%, the inoculation concentration was 0.60g / L, and the culture was carried out for 65 hours (among which the 5L bioreactor was cultured in 34 hours, 31 hours in a 1L open-plate photobioreactor). The results showed that the cell density ( ) reached a maximum of 15.36 g / L at 34 hours of culture, and the contents of algal body protein ( ○ ) and chlorophyll ( ) reached the highest at 65 hours of culture, which were 54.78% and 31.23 mg / gDcw respectively (see Figure 5 ).

[0076] KNO3: 9.25 Glucose: 30

[0077] KH 2 PO 4 : 0.7 Na 2 HPO 4 ·12H 2 O: 1.84

[0078] MgSO 4 ·7H 2 O: 0.7CaCl2 : 0.142

[0079] FeSO 4 ·7H 2 O: 0.02

[0080] Trace elements: 1ml

[0081] Trace element formula (g / L): H 3 BO 3 11.42 ZnSO 4 ·7H 2 O 8.82

[0082] MnCl 2 ·H 2 O 1.42 (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.8707

[0083] ...

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Abstract

The invention relates to a culture media composition suitable for cultivating high-density high-quality ordinary chlorella, wherein the culture medium mainly comprises KNO3 7-11g / l, glucose 25-35g / l and small amount of inorganic salt, minor elements and water. The culture medium can substantially improve the quality of globule algae.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a medium composition suitable for high-density and high-quality cultivation of Chlorella vulgaris and a method for culturing Chlorella vulgaris with high-density and high-quality. Background technique [0002] Chlorella has a long research history, among which Chlorella vulgaris is one of the most studied algal species, and it is also one of the algal species commonly used in commercial production. [0003] At present, there are two commonly used cultivation methods for Chlorella: open outdoor large pond photoautotrophic cultivation and fermentor heterotrophic cultivation. Open outdoor large-pond culture is currently a commonly used method for commercial large-scale production. Although its cost is low, its development is greatly limited due to the shortcomings of low cell density, easy pollution, and large footprint. Heterotrophic culture is basically at the stage of laboratory resea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12A23K1/00A23K10/30
Inventor 李元广李兴武王伟沈国敏魏鸿刚安洋
Owner JIAXING ZEYUAN BIOLOGICAL PROD
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