Method for detecting microalgae oil

A technology of microalgae oil and detection method, which is applied in the preparation of test samples, fluorescence/phosphorescence, material excitation analysis, etc. The effect of reducing the total consumption time

Inactive Publication Date: 2011-04-27
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention solves the technical problem that the weighing method require

Method used

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  • Method for detecting microalgae oil
  • Method for detecting microalgae oil
  • Method for detecting microalgae oil

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Experimental program
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specific Embodiment approach 1

[0010] Embodiment 1: The detection method of microalgae oil in this embodiment is carried out according to the following steps: 1. Dilute the microalgae culture solution to 0.05g / L~0.5g / L to obtain the dilution; 2. Take 10mL Add 100 μL of Nile Red solution to the diluent in step 1, stain in the dark, select 400-600 nm as the excitation wavelength, measure the fluorescence intensity between the emission wavelength 450-700 nm, and record the fluorescence intensity as X1; Centrifuge the diluted solution for 10 minutes at a temperature of 4°C and a rotational speed of 10,000 r / min, take the supernatant as a blank, take 10 mL of the supernatant, add 100 μL of Nile Red solution, and stain in the dark, choose 400-600 nm As the excitation wavelength, measure the fluorescence intensity between the emission wavelength 450-700nm, and record the fluorescence intensity as X2; 4. Take the diluent in step 1, select 400-600nm as the excitation wavelength, and measure the fluorescence intensity...

specific Embodiment approach 2

[0011] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the solvent used to dilute the solution containing microalgae in step 1 is distilled water, tap water, deionized water, ultra-clean water or mineral water. Other steps and parameters are the same as in the first embodiment.

specific Embodiment approach 3

[0012] Specific embodiment three: this embodiment is different from specific embodiment one or two in that: the solvent of the Nile red solution described in step two is acetone, ethanol, propanol, dioxane, tetrahydrofuran or methyl ethyl ketone. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention provides a method for detecting microalgae oil, belonging to the field of oil detection. The method solves the technical problems that the needed sample is large and the consumed time is long in the existing weighing method. The method comprises the following steps: 1, diluting microalgae culture solution to obtain diluent; 2, detecting the fluorescence intensity of the dyed diluent; 3, centrifuging the diluent, and detecting the fluorescence intensity of the dyed supernate; 4, detecting the fluorescence intensity of the undyed diluent; and 5, computing the maximum difference of the fluorescence intensity, and computing the content of the microalgae oil according to a fluorescence intensity-oil content standard curve. The method is applied to detecting the unicellular algae such as the chlorella, the scenedesmus, the spirulina, the diatom, the dinoflagellate, the chrysophyceae, the euglenophyta, the stonewort and the like, and is applied to detecting the macro algae which is preprocessed by cell discruption.

Description

technical field [0001] The invention belongs to the field of grease detection. Background technique [0002] Microalgae energy is a new energy source that has emerged in recent years, and the use of microalgae oil to produce biodiesel has attracted widespread attention. Biodiesel has the characteristics of "carbon neutral", so it can alleviate the global warming effect, and its pollutant emission level is lower than that of fossil diesel. In recent years, the production of biodiesel has increased significantly, but the high cost of biodiesel and insufficient supply of raw materials have hindered the further promotion and application of biodiesel. As a raw material for biodiesel, microalgae oil has been proposed and verified by experiments, but limited by the cost of cultivation and collection, it is still unable to compete with petrochemical energy. [0003] One of the ways to reduce the cost of microalgae biodiesel is to increase the lipid content of harvested algae cells...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/30
Inventor 冯玉杰李超刘佳张大伟
Owner HARBIN INST OF TECH
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