Method for culturing chlorella

A technology of chlorella and culture medium, which is applied in the field of microalgae biology, can solve problems such as the lack of corresponding improvement in oil accumulation efficiency, raw material limitations, and increased cultivation costs, so as to achieve high-quality and high-density culture of microalgae and solve pollution problems , The effect of cultivating high efficiency

Active Publication Date: 2012-01-11
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

[0005] The second is the heterotrophic culture method, which is to provide artificial carbon sources during the culture process, such as glucose. Obviously, glucose is a heterotrophic carbon source, which is a quick-acting carbon source and can increase the growth rate of microalgae, but its oil accumulation efficiency is not high. There is no corresponding improvement, and at the same time, this type of carbon source will inevitably increase the cost of cultivation, which will be greatly limited by raw materials
[0009] Due to the addition of high-priced glucose as an organic carbon source in the heterotrophic culture process, the existing two-step culture method has problems such as poor economy of heterotrophic carbon source and unsatisfactory culture efficiency.

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  • Method for culturing chlorella

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Effect test

Embodiment 1

[0033] The starting algae species is a kind of autotrophic microalgae isolated from nature, which is identified as Chlorella vulgaris after separation and purification in the laboratory. The laboratory number is FY1#. The medium used is the basic medium.

[0034] Table 1

[0035] NaNO 3

[0036] The chlorella species were transferred from the inclined plane to the shake flask and cultivated under the conditions of 30°C, 100rpm, 30001x light and air bath, and the experimental data under the autotrophic culture conditions were obtained. Cultivate for 10 days, analyze the concentration of algae cells in the test shaker flask, measure the number of live cells therein with a cell counter, and analyze the total percentage of oil and fat in the algae cells under dry weight conditions with Soxhlet extraction.

Embodiment 2

[0038] The starting algae species is FY1# autotrophic Chlorella, and the culture condition is to add heterotrophic carbon source to the basic medium, add 0.02mmol / L Na 2 SO 3 and the bacterial slag pretreatment solution with a concentration of 3% (v / v); the expression of the concentration of the added substance here is based on the total volume of the medium, that is, the content of the added substance accounts for the total culture solution after the preparation of the culture solution is completed. Percentage by volume or number of moles or mass, etc., the same is true for other embodiments.

[0039] Among them, the source of bacterial residue is Klebsiella pneumoniae, which grows anaerobically. This bacterium uses glycerol as the only substrate and grows under anaerobic culture conditions. It can ferment and produce 1,3-propanediol. After cooking for 40 minutes, filter the filter cake of bacterial residue at normal temperature to obtain the filter cake of bacterial residue...

Embodiment 3

[0043] The domesticated algae species FY1X-9# obtained on the basis of Example 2 was subjected to an investigation experiment of adding glucose as a carbon source. The amount of glucose added was 30g / L, and other culture conditions were consistent with the heterotrophic domestication conditions. Cultured for 5 days, Analyze the concentration of algae cells in the test shaker flask, measure the number of viable cells therein with a cell counter, and analyze the percentage of oil and fat contained in the algae cells under the condition of dry weight by Soxhlet extraction.

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Abstract

The invention discloses a method for culturing chlorella. The invention provides a method for realizing mixed cultivation by taking a microorganism residue treatment solution as a main heterotrophic carbon source, which is characterized in that the mixed cultivation is carried out by conducting domestication cultivation on chlorella autotrophica under a heterotrophic condition through coupling the two processes of autrophism and heterotrophism; and after the mixed cultivation is ended and flocculated separation on a chlorella solution is ended, the chlorella solution is extracted to obtain grease, and remaining chlorella cells and microorganism residues which are not utilized can be used for further microalgae cultivation through a pretreatment process provided by the invention. In the method for culturing the chlorella, the coupling is realized on the two processes of the autrophism and the heterotrophism by using a cheap carbon source as an organic carbon source. A new way is provided for the technology for growing the grease through culturing microalgae on a large scale by the technical route of the invention.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for cultivating microalgae by polyculture fermentation using cheap carbon sources. Background technique [0002] Microalgae are rich in protein, polysaccharides, unsaturated fatty acids and other nutrients (such as spirulina), which can be used in food, medicine and energy; they can accumulate fatty acids in large quantities, and the fatty acid content of some microalgae can account for 30% to 60% of dry weight. The use of microalgae to accumulate oil resources has become the most popular research field in the development of renewable resources using solar energy. Not only has strong market potential, but also has extraordinary social value. [0003] However, most wild microalgae do not grow fast and have limited ability to fix carbon dioxide. To achieve large-scale production, it will consume a lot of manpower and material resources with little gain....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王领民金平师文静李晓姝王崇辉佟明友黎元生
Owner CHINA PETROLEUM & CHEM CORP
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