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Chlorella, cultivating method and application thereof in producing biodiesel

A technology of biodiesel and cultivation method, which is applied in the field of chlorella and its cultivation method and its application in biodiesel production, can solve the problems of high production cost and achieve the effect of low cost

Inactive Publication Date: 2009-10-28
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the existing microalgae used as raw materials for biodiesel are mostly separated in seawater, and are mainly cultivated in medium with high nutrient concentration, and the production cost is too high

Method used

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  • Chlorella, cultivating method and application thereof in producing biodiesel
  • Chlorella, cultivating method and application thereof in producing biodiesel
  • Chlorella, cultivating method and application thereof in producing biodiesel

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Chlorella algae species YJ1 was inoculated into the secondary effluent of Beijing Gaobeidian Sewage Treatment Plant with an initial inoculation density of 5×10 4 pcs mL -1 . The light-dark ratio is 12h:12h, the temperature is 25°C, the relative humidity is 75%, and the light intensity is 1100lux, and it is cultivated for about 18 days. At different times of cultivation, the growth curve and oil content of the chlorella are measured. Centrifuge at 6000rpm for 10min after the cultivation, and harvest Chlorella.

[0026] The result is as figure 1 As shown, the maximum algal density of chlorella growing in the secondary effluent of the urban sewage can be as high as 10 7 pcs mL -1 ; The oil percentage can be as high as 43%.

Embodiment 2

[0028] Chlorella algae species YJ1 was inoculated into the secondary effluent of Qinghe Wastewater Treatment Plant with an initial inoculation density of 1×10 4 pcs mL -1 . The chlorella was cultured for about 20 days under the conditions of a light-dark ratio of 14h:10h, a temperature of 27°C, a relative humidity of 70%, and a light intensity of 1300 lux, and the growth curve and oil content of the chlorella were measured. Centrifuge at 6000rpm for 10min after the cultivation, and harvest Chlorella.

[0029] The result is as figure 2 As shown, the maximum algal density of chlorella growing in the secondary effluent of the urban sewage can be as high as 10 6 pcs mL -1 ; The oil percentage can be as high as 43%.

Embodiment 3

[0031] Chlorella species YJ1 was inoculated into the secondary effluent of Jiuxianqiao Sewage Treatment Plant with an initial inoculation density of 1×10 5 pcs mL -1 . The chlorella was cultured for about 21 days under the conditions of a light-dark ratio of 13h:11h, a temperature of 22°C, a relative humidity of 80%, and a light intensity of 800 lux, and the growth curve and oil content of the chlorella were measured. Centrifuge at 6000rpm for 10min after the cultivation, and harvest Chlorella.

[0032] The result is as image 3 As shown, the maximum algae density of chlorella growing in the secondary effluent of urban sewage can reach 10 7 pcs mL -1 ; The oil percentage can be as high as 43%.

[0033] The chlorella obtained in the above examples can be used to produce biodiesel through transesterification.

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Abstract

The invention discloses a chlorella which belongs to the technical field of producing biodiesel and a cultivating method thereof. The chlorella is named as Chlorella YJ1, and the registration number of the spawn preservation is CGMCC No. 3037. The invention also discloses the application of the chlorella in producing biodiesel. Second-grade effluent water of municipal sewage is taken as the culture medium to grow the chlorella without adding nutrient elements such as nitrogen, phosphorus and the like, thus reducing the cost of producing biodiesel and lightening the burden of water resource. The chlorella is used for establishing the process of municipal sewage deep treatment, and the biodiesel can be obtained at the same time of the sewage treatment, which is a sewage recycling and biodiesel producing technology with eco-friendliness and low cost.

Description

technical field [0001] The invention belongs to the technical field of biodiesel production, and in particular relates to a chlorella and its cultivation method and its application in biodiesel production. Background technique [0002] Fossil energy is facing the plight of depletion, and it is imperative to find sustainable renewable and environmentally friendly new energy sources. Microalgae has the characteristics of fast growth rate, short harvest period, and high photosynthetic utilization rate. The use of microalgae as a raw material for biodiesel has attracted more and more attention. At present, most of the existing microalgae used as raw materials for biodiesel are separated in seawater, and are mainly cultivated in medium with high nutrient concentration, and the production cost is too high. Therefore, it is necessary to combine the production of biodiesel with the production of other by-products (such as animal feed, fertilizer, etc.) or service behavior (use of w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C10G3/00C12R1/89
CPCY02E50/13Y02E50/10Y02P30/20
Inventor 胡洪营杨佳李鑫
Owner TSINGHUA UNIV
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