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Bait formula for enhancing nutrition of meretrix lamarckii at planktonic larva phase

A planktonic larvae and nutrition-enhancing technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of artificial propagation of the axe clams, immature breeding technology, inhibiting the development of planktonic larvae of seedlings, and insufficient nutrition supply of bait, etc. Effects of animal immune function, increasing feed utilization, improving immunity and anti-stress ability

Inactive Publication Date: 2018-12-28
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the artificial propagation and breeding technology of Meretrix axetrix is ​​immature, the amount of natural resources is scarce, and the reports on the production, development and basic research of Meretrix axetrix are very scarce. The key to the artificial breeding technology of Meretrix axetrix lies in seedling breeding, whether the seedling breeding is successful or not It is related to the high-efficiency cultivation and healthy development of Meretrix meretrix. However, there is a situation of insufficient nutrition in the supply of bait in the planktonic larval stage in artificial seedling culture. The problem of basic bait formula has not been completely solved, and the conventional use of single algae feeding without formula makes the nutrition insufficient and inhibits the development of planktonic larvae in seedlings

Method used

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  • Bait formula for enhancing nutrition of meretrix lamarckii at planktonic larva phase

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Embodiment 1

[0022] A bait formula for nutritional enhancement of planktonic larvae of Meretrix meretrix, the bait formula contains marine rhodotorula mother liquor prepared by the following method: after the bacteria are activated, they are inoculated on a liquid medium for propagation and cultivation to obtain a secondary After planting, the secondary species is fermented and cultivated, wherein a liquid medium containing oleic acid glyceride and sodium butyrate needs to be supplemented during the fermenting and cultivating process. Marine yeast has high protein content, generally 30-50% of dry cell weight, and contains 10 essential amino acids required by shellfish and crustaceans, as well as digestive enzymes, vitamins, minerals, and growth-promoting factors produced during growth As well as zymosan, these ingredients can improve the digestion and absorption rate of plankton, increase its utilization rate of bait, and stimulate the body's immune response to improve its immune and diseas...

Embodiment 2

[0031] A bait formula for nutritional enhancement of planktonic larvae of Meretrix meretrix, the bait formula contains marine rhodotorula mother liquor prepared by the following method:

[0032] 1) Activation of strains: Inoculate the strains in cold storage on the slant medium, and cultivate them at 30°C for 46 hours to obtain them;

[0033] 2) The multiplication and cultivation steps are as follows: inoculate the activated strains on the liquid medium, vibrate and culture in a constant temperature shaker at a temperature of 30°C and a rotation speed of 180r / min for 50 hours to obtain first-grade seeds, and then inoculate a Grade seeds were inoculated on the liquid medium with an inoculation amount of 11% (V / V), and were cultivated for 28 hours at a temperature of 30°C and a rotating speed of 180r / min. During the cultivation, sterile air was passed into the medium for deep layer Inflated, the ventilation volume is 200m 3 / h, you can get the secondary seed;

[0034] 3) The f...

Embodiment 3

[0039] A bait formula for nutritional enhancement of planktonic larvae of Meretrix meretrix, the bait formula contains marine rhodotorula mother liquor prepared by the following method:

[0040] 1) Activation of strains: Inoculate the strains in cold storage on the slant medium, and cultivate them at 28°C for 48 hours to obtain them;

[0041] 2) The multiplication and cultivation steps are as follows: inoculate the activated strains on the liquid medium, vibrate and cultivate in a constant temperature shaker at a temperature of 28°C and a rotation speed of 200r / min for 48 hours to obtain first-grade seeds, and then inoculate a Grade seeds are inoculated on the liquid medium with an inoculation amount of 10% (V / V), and cultivated for 32 hours at a temperature of 28°C and a rotating speed of 200r / min. During the cultivation, sterile air is passed into the medium for deep layer Inflated, the ventilation volume is 180m 3 / h, you can get the secondary seed;

[0042] 3) The fermen...

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Abstract

The invention discloses a bait formula for enhancing nutrition of meretrix lamarckii at a planktonic larva phase. The bait formula comprises the following raw materials in parts by weight: 3-7 parts of a chlorella mother liquid, 0.5-0.8 part of a isochrysis galbana parke mother liquid, 0.6-0.9 part of a chaetoceros mulleri mother liquid, 0.05-0.6 part of a platymonas subcordiformis mother liquid and 0.02-0.06 part of a rhodotorula benthica mother liquid. The bait formula for enhancing nutrition of meretrix lamarckii at the planktonic larva phase, which is disclosed by the invention, has comprehensive and sufficient nutrition, is good in palatability, excellent in food calling and intake effect, capable of meeting requirements of enhanced nutrition and high-metamorphosis survival rates at the larva phase, improving the immunity and the stress resistance of zooplankton, improving disease and insect resistance of the zooplankton and purifying water, and has rich bacterial with high activity and metabolite with great gain, and development by metamorphosis of planktonic larvae can be accelerated.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a bait formula for nutritional strengthening of the planktonic larvae of Meretrix meretrix. technical background [0002] Meretrix meretrix belongs to the phylum Molluscs, belonging to the class Petalbranchia, clam order, clam family, clam genus shellfish. Meretrix meretrix is ​​a warm-water marine bivalves, mainly concentrated in Japan and the South China Sea. It is distributed in the coastal areas of Guangdong, South China Sea, Guangxi and other provinces in my country. It is usually buried in the subtidal zone to the sandy bottom of the shallow sea with a water depth of 20m. Meretrix meretrix has a large adult body, delicious meat, and rich nutrition. It is deeply loved by the masses. It is a shellfish with high economic value in the southeast coast of my country and has broad prospects for breeding and development. Meretrix meretrix has always relied on the harvesting of natural r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/16
CPCA23K10/16A23K50/80
Inventor 叶莹莹傅泽钦冯鉴童李继姬郭宝英
Owner ZHEJIANG OCEAN UNIV
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