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Detection method for distribution of carotene in Isochrysis galbana based on Raman spectroscopy

A Raman spectroscopy and carotene technology is applied in the field of detection of carotene distribution in dinoflagellates, which can solve the problems of time-consuming, labor-intensive, and complicated operation, so as to simplify the operation steps, shorten the detection time, and avoid unskilled operation. Effect

Active Publication Date: 2013-11-20
ZHEJIANG UNIV
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Problems solved by technology

[0005] The invention provides a method for detecting the distribution of carotene in Isochrysis algae based on Raman spectroscopy, which solves the problem that the existing detection method requires dyeing or complex chemical treatment of the sample, and the operation is relatively cumbersome, time-consuming and labor-intensive The problem

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  • Detection method for distribution of carotene in Isochrysis galbana based on Raman spectroscopy
  • Detection method for distribution of carotene in Isochrysis galbana based on Raman spectroscopy
  • Detection method for distribution of carotene in Isochrysis galbana based on Raman spectroscopy

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Embodiment Construction

[0025] The present invention is further explained below in conjunction with specific examples.

[0026] A certain concentration of Isochrysis samples was taken, and the Renishaw confocal Raman spectrometer (inVia–Reflex 532 / XYZ) was used to obtain the raw information of the Raman spectrum of the live Isochrysis algae fluid samples. The prepared algae liquid slice is fixed on the stage under the objective lens of the micro-Raman spectrometer, and the laser beam is focused on the surface of the sample through the 50X objective lens. The exposure time is set to 1s, the laser intensity is 1mv, and the accumulation times are once. The whole experiment process was carried out under the condition of constant temperature (about 25°C). Figure 1a is the original Raman spectrum of a certain dinoflagellate algae liquid sample.

[0027] Since the original Raman spectrum is greatly disturbed by fluorescence, the generation of fluorescence will cover the Raman signal. Therefore, the method...

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Abstract

The invention discloses a detection method for distribution of carotene in Isochrysis galbana based on Raman spectroscopy. The detection method comprises the following steps: acquiring original Raman spectroscopic information of a living Isochrysis galbana liquid sample by using a Raman spectrometer; with carotene in pigment as an example, acquiring the spectral line of a standard carotene product by using the Raman spectrometer; preprocessing obtained original Raman data of the Isochrysis galbana liquid sample and identifying spectral peak positions of corresponding Raman spectral peaks of the Isochrysis galbana liquid sample on the basis of the spectral line of the standard carotene product; and scanning the Isochrysis galbana liquid sample in a certain area and subjecting Raman intensity values of characteristic spectral peaks corresponding to carotene to integration so as to obtain a chemical image of distribution of carotene in Isochrysis galbana liquid. The detection method provided by the invention overcomes the problems of need for dyeing or complex chemical treatment of a sample, complicated operation, consumption of time and labor, etc. of conventional detection methods.

Description

technical field [0001] The invention relates to the technical field of chemical images of microalgae pigment distribution, in particular to a method for detecting the distribution of carotene in Isochrysis algae based on Raman spectroscopy. Background technique [0002] Microalgae are a class of aquatic (or terrestrial, aerial, symbiotic) lower plants with diverse phylogenies, small individuals, usually single cells or groups, and capable of photosynthesis (a small part is heterotrophic growth). It is a microorganism capable of photosynthesis, using sunlight, water and CO 2 Synthesize the substances you need. At present, the identification, classification and biochemical analysis of algae have become one of the research hotspots in marine biology. [0003] Isochrysis belongs to Chrysophyta, Isochrysis, and Isochrysis. The cell size is generally 6-7μm×5-6μm, and there are two equal-length flagella on the cell. Isochrysis has the advantages of fast reproduction speed, no ce...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 邵咏妮何勇李晓丽罗溜彬魏萱
Owner ZHEJIANG UNIV
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