Outdoor large-area cultivating method for Isochrysis galbana H29 containing rich DHA

Abstract

The invention relates to method for cultivating Isochrysis galbana Parke H29 rich in DHA outdoor in large area. The method is characterized preparing culture fluid, conserving seeds, determining culture conditions, training with salt, installing automatic temperature controlling system, expanding seeds gradually, culturing in the outdoor cultivating pool, harvesting, drying, and inspecting the final product. The superiorities of the invention are low materials and energy consumption, high production efficiency, low cost, high achievement ratio, short cultivation period, and industrialized production.

Description

(1) Technical field:

[0001] The invention relates to an algae cultivation method, in particular to an outdoor large-area cultivation method of Isochrysis globosa H29 rich in DHA. (two) background technology:

[0002] DHA (docosahexaenoic acid) is a highly unsaturated fatty acid, which is an important nutrient for human brain development. DHA is mainly derived from marine fish oil, but there are some shortcomings in the production of DHA from fish oil, such as the DHA content in fish oil is affected by seasons, regions, species, etc., and fish resources are becoming less and less; the produced DHA is not pure enough and easy to oxidize, which has Special fishy smell, further purification and refining process is complicated, and the product cost is high. With the elucidation of the physiological, nutritional and pharmacological effects of polyunsaturated fatty acids, the human demand for polyunsaturated fatty acids such as EPA and DHA is increasing, and extraction from fish o...