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Outdoor large-area cultivating method for Isochrysis galbana H29 containing rich DHA

A technology of Isochrysis globosa and a breeding method, which is applied in the field of outdoor large-area cultivation of Chrysopsis dinoflagellate H29, can solve the problem of low DHA content, achieve the effects of less material and energy consumption, shortened breeding cycle, and high production efficiency

Inactive Publication Date: 2009-04-01
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the DHA content is not high when H24 is cultured at high temperature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: a kind of outdoor large area cultivation method of DHA-rich Isochrysis globosa H29, is characterized in that it is made of following steps:

[0021] (1) Culture medium formula: adopt conventional f / 2 formula;

[0022] The f / 2 formula is:

[0023] NaNO 4 (74.8mg / l) NaH 2 PO 4 (4.4mg / l)

[0024] B 1 (0.1mg / l) B 12 (0.0005mg / l)

[0025] FeC 6 h 5 o 7 ·H 2 O (3.9mg / l)

[0026] ZnSO 4 4H 2 O(23×10 -3 mg / l)

[0027] MnCl 2 4H 2 O(178×10 -3 mg / l)

[0028] CuSO 4 ·5H 2 O(10×10 -3 mg / l)

[0029] Na 2 MoO 4 ·H 2 O(7.3×10 -3 mg / l)

[0030] CoCl 2 ·H 2 O(12×10 -3 mg / l)

[0031] Na 2 EDTA (4.35mg / l)

[0032] (2) Seed preservation: carry out seed preservation in the seed preservation box of the laboratory;

[0033] (3) Determine the culture conditions:

[0034] Cultivate in the laboratory under the following conditions: temperature 16°C, salinity 16, light: 3000Lux

[0035] (4) Salinity domestication:

[0036] Step-by-step salinity ...

Embodiment 2

[0044] Embodiment 2: a kind of outdoor large area cultivation method of DHA-rich Isochrysis globosa H29, is characterized in that it is made of following steps:

[0045] (1) Culture solution formula: adopt conventional f / 2 formula, with embodiment 1;

[0046] (2) species preservation: with embodiment 1;

[0047] (3) Determine the culture conditions:

[0048] Cultivate in the laboratory at a temperature of 22°C, a salinity of 28, and a light of 8000 Lux;

[0049] (4) Salinity acclimatization: carry out step by step salinity acclimatization in the laboratory: temperature 22 ℃, cultivate under salinity 40;

[0050] (5) Install an automatic temperature control system: In the installation of an automatic temperature control system in a 50-square-meter breeding pond, the temperature of the water body in the control pond is 13.0°C--28.0°C;

[0051] (6) Gradually expand the species:

[0052] 1 liter → 70 liters → 6 square meters → 50 square meters → 300 square meters, scale up and...

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Abstract

The invention relates to method for cultivating Isochrysis galbana Parke H29 rich in DHA outdoor in large area. The method is characterized preparing culture fluid, conserving seeds, determining culture conditions, training with salt, installing automatic temperature controlling system, expanding seeds gradually, culturing in the outdoor cultivating pool, harvesting, drying, and inspecting the final product. The superiorities of the invention are low materials and energy consumption, high production efficiency, low cost, high achievement ratio, short cultivation period, and industrialized production.

Description

(1) Technical field: [0001] The invention relates to an algae cultivation method, in particular to an outdoor large-area cultivation method of Isochrysis globosa H29 rich in DHA. (two) background technology: [0002] DHA (docosahexaenoic acid) is a highly unsaturated fatty acid, which is an important nutrient for human brain development. DHA is mainly derived from marine fish oil, but there are some shortcomings in the production of DHA from fish oil, such as the DHA content in fish oil is affected by seasons, regions, species, etc., and fish resources are becoming less and less; the produced DHA is not pure enough and easy to oxidize, which has Special fishy smell, further purification and refining process is complicated, and the product cost is high. With the elucidation of the physiological, nutritional and pharmacological effects of polyunsaturated fatty acids, the human demand for polyunsaturated fatty acids such as EPA and DHA is increasing, and extraction from fish o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12A01G7/00A01D44/00A01G33/00
CPCY02A40/80
Inventor 王学魁孙之南曹春晖刘文岭马若欣张波吕本松高清潭王云生张桂香
Owner TIANJIN UNIV OF SCI & TECH
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