Method for improving culture density of isochrysis galbana

Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a method for improving the culture density of isochrysis galbana. The method comprises the steps: activatinga marinobacter aquaeolei strain; culturing a marinobacter aquaeolei solution; carrying out sterile treatment on isochrysis galbana species; and producing an isochrysis galbana seed culture solution,wherein the density of isochrysis galbana is more than or equal to 10*10<5> / mL, and according to formal culture production of isochrysis galbana, the density of isochrysis galbana is 1.5*10<7> cell / mLor above. The isochrysis galbana and marinobacter aquaeolei co-culture system can generate a synergistic effect, so that the components of a culture medium can be fully utilized, and the isochrysis galbana and the marinobacter aquaeolei simultaneously benefit from each other and promote co-growth due to the physiological and ecological characteristics of the isochrysis galbana and the marinobacter aquaeolei. Under the condition of mixed culture of the isochrysis galbana and the marinobacter aquaeolei, the isochrysis galbana can continuously and quickly grow, the biomass higher than that of aconventional method is finally obtained, and effective technical support can be provided for related aquaculture baits and DHA-obtaining algae.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for increasing the culture density of Isoflagellates. Background technique [0002] With the deepening of research on polyunsaturated fatty acids (Polyunsaturated Fatty Acids, PUFAs), people have gradually realized that PUFAs, as a class of substances with unique biological activities, play a very important role in physiological health, nutritional supplements and drug development. . Among them, ω-3 PUFAs represented by α-Linolenic Acid (ALA), Eicosapentaenoic (EPA) and Docosahexaenoic Acid (DHA) are involved in brain development. , preventing cardiovascular disease, and improving vision have become the focus of current research. [0003] However, at present, PUFAs are mainly derived from marine fish, and their sources are very poor, which is far from meeting the market demand. Therefore, it is urgent to find a more sustainable and stable source of ...

AI Technical Summary

Problems solved by technology

There are three main problems in extracting PUFAs from fish oil: (1) Unstable yield: the content of PUFAs in fish oil fluctuates greatly with different limiting factors such as fish species, fishing season, temperature and climate

(2) Low product quality: the hydrogenation treatment process reduces the production of PUFAs in fish oil, and contains fishy smell, which limits the application of the product in the food market

(3) High refining cost: For cholesterol, fat-soluble vitamins and up to 80% saturated fatty acids that are difficult to remove in fish oil, the process of purifying PUFAs is complicated, thereby increasing the cost of refining

However, because the growth density has been maintained at a low level, the upper limit of the growth density cannot be broken through, resulting in low production capacity and high cultivation costs, which cannot provide economically competitive raw materials for industrial production of DHA

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