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Method for reducing isochrysis-galbana adherence and increasing growth amount

A technology of isochrysis globosa, growth amount, applied in microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problems of insufficient light, harsh living environment, adherence to the wall, etc.

Active Publication Date: 2019-02-19
杭州园泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the cultivation process of Isoflagellates, due to gravity, uneven aeration, and the physiological characteristics of Isoflagellates, etc., the inner wall of the culture device or the objects in contact with the algae liquid often appear adherence
Slight adherence will not affect the growth of algal cells, but once large-scale adherence occurs, it will produce a vicious circle and form adverse effects, making it difficult for algal cells to absorb nutrients, poor living environment and insufficient light. It is a technical problem that we need to solve to avoid the occurrence of large-scale adherence in cultured dinoflagellates

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for reducing Isochrysis globosa to stick to the wall and improving growth, it comprises the steps:

[0029] Inoculate the Isochrysis globosa that has been cultivated to the logarithmic growth phase into the seed tank containing the seed medium, and the initial inoculation density is 1 × 10 5 seeds / ml, light intensity 5000lux, culture at 22°C, light-to-dark ratio 18:6, ventilation rate 0.2vvm, culture for 48 hours, and collect seed solution;

[0030] The components of the seed culture medium are: sodium nitrate 1.5g / L, sodium silicate 0.5g / L, sodium chloride 0.3g / L, ammonium sulfate 0.2g / L, potassium dihydrogen phosphate 0.1g / L, VB 1 0.5mg / L, VB 12 0.1mg / L;

[0031] Inoculate the seed liquid into the reaction tank containing the culture liquid according to the inoculum amount of 5%, then add 200mg / L amino acid, cultivate at 22°C, the light-dark ratio is 12:12, and the ventilation rate is 0.5vvm; after culturing for 72 hours, collect Algal cells; the amino...

Embodiment 2

[0034] The method for reducing Isochrysis globosa to stick to the wall and improving growth, it comprises the steps:

[0035] Inoculate the Isochrysis globosa that has been cultivated to the logarithmic growth phase into the seed tank containing the seed medium, and the initial inoculation density is 1 × 10 5 seeds / ml, light intensity 5000lux, culture at 22°C, light-to-dark ratio 18:6, ventilation rate 0.2vvm, culture for 48 hours, and collect seed solution;

[0036] The components of the seed culture medium are: sodium nitrate 1.5g / L, sodium silicate 0.5g / L, sodium chloride 0.3g / L, ammonium sulfate 0.2g / L, potassium dihydrogen phosphate 0.1g / L, VB 1 0.5mg / L, VB 12 0.1mg / L;

[0037] Inoculate the seed liquid into the reaction pool containing the culture liquid according to the inoculum amount of 5%, then add 100mg / L amino acid, cultivate at 22°C, the light-dark ratio is 12:12, and the ventilation rate is 0.5vvm; after culturing for 48 hours, collect Algal cells; the amino...

Embodiment 3

[0040] The method for reducing Isochrysis globosa to stick to the wall and improving growth, it comprises the steps:

[0041] Inoculate the Isochrysis globosa that has been cultivated to the logarithmic growth phase into the seed tank containing the seed medium, and the initial inoculation density is 1 × 10 5 seeds / ml, light intensity 5000lux, culture at 22°C, light-to-dark ratio 18:6, ventilation rate 0.2vvm, culture for 48 hours, and collect seed solution;

[0042] The components of the seed culture medium are: sodium nitrate 1.5g / L, sodium silicate 0.5g / L, sodium chloride 0.3g / L, ammonium sulfate 0.2g / L, potassium dihydrogen phosphate 0.1g / L, VB 1 0.5mg / L, VB 12 0.1mg / L;

[0043] Inoculate the seed liquid into the reaction tank containing the culture liquid according to the inoculation amount of 5%, then add 150mg / L amino acid, cultivate at 22°C, the light-dark ratio is 12:12, and the ventilation rate is 0.5vvm; after culturing for 60 hours, collect Algal cells; the am...

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Abstract

The invention belongs to the technical field of algae, and discloses a method for reducing isochrysis-galbana adherence and increasing the growth amount. The method includes the following steps that an isochrysis-galbana seed solution is inoculated to a reaction pool containing a culture solution according to the inoculation amount of 5% to 10%, then 100 mg / L-200 mg / L amino acid is added, and themixture is cultured at the temperature of 20 DEG C to 22 DEG C, wherein the light-dark ratio is 12:12, and the ventilation rate is 0.4 vvm to 0.5 vvm; culturing is carried out for 48 h to 72 h, and algal cells are collected. By means of the method, the isochrysis-galbana adherence phenomenon can be obviously reduced, and the biomass of the algal cells is increased.

Description

technical field [0001] The invention belongs to the technical field of algae, in particular to a method for reducing dinoflagellate algae adherence to the wall and increasing growth. Background technique [0002] Isochrysis globosa is a single-celled microalgae belonging to the order Isoflagellates, Isochrysis, and the genus Isochrysis. In addition, it still contains more carotenoids, is photoautotrophic, has a simple culture method, low cost, fast proliferation rate, and is rich in unsaturated fatty acids in the cells. [0003] Most of the existing research focuses on how to increase the production of unsaturated fatty acids in algae cells. For example, the Chinese invention patent "CN201410597451" discloses a method for increasing the DHA content in Chrysophyta, which includes adding a certain amount of Glycerol, DHA production can be increased, while increasing the content of other fatty acids. There are few studies on the growth status of Isochrysis globosa in the earl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38C12R1/89
CPCC12N1/12C12N1/38
Inventor 胡叶飞张菊萍董国强张宏杰金荣培
Owner 杭州园泰生物科技有限公司
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