Method for finishing bacterium fixation by utilizing polyvinyl alcohol-borate secondary crosslinking

A polyvinyl alcohol, secondary cross-linking technology, applied in the direction of being fixed on/in an organic carrier, can solve the problems of water stability reduction, bacterial protection, volume expansion, mutual adhesion of particles, etc., to solve the problems of adhesion and water solubility. The effect of expansion, good elasticity and simple operation

Inactive Publication Date: 2013-07-10
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problems, the object of the present invention is to use the method of secondary cross-linking to adjust the pH to solve the problems of mutual adhesion, volume expansion, water stability reduction and bacterial protection of the particles prepared by the polyethanol-boric acid method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Selection and processing of filler carrier

[0020] Select quartz sand or zeolite as the bacterial filler carrier, and keep the filler carrier with a particle size of 0.8-1.0mm through sieving and screening, take it out after soaking in bacterial liquid culture medium for 3 days, and put it in a constant temperature oven at 60°C to dry to ensure the surface of the filler carrier If it is not wet, take out 10L of the dried filler carrier and cool it to room temperature for later use.

[0021] (2) Acquisition of bacterial embedding solution

[0022] Weigh 80g of polyvinyl alcohol, add an appropriate amount of water, heat to dissolve, mix with 50ml of bacterial concentrate after cooling to room temperature, add 40g of calcium carbonate, stir well, and make 1L of bacterial embedding solution.

[0023] (3) Mixing of bacterial embedding solution and filler carrier

[0024] Pour the bacterial embedding solution into the prepared filler carrier in small quantities in batc...

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Abstract

The invention relates to a method for finishing bacterium fixation by utilizing polyvinyl alcohol-borate secondary crosslinking, belonging to the field of water treatment. The method comprises the steps of: after mixing polyvinyl alcohol and bacteria in proportion, putting the mixture into saturated borate solution and carrying out first crosslinking fixation, after the fixation, taking out supernate and regulating pH of the supernate, carrying out second crosslinking fixation on the polyvinyl alcohol by utilizing the supernate in which the pH is regulated, and cleaning the polyvinyl alcohol after the fixation, so as to finish the whole fixation process. The method has the advantages that the damage to the bacteria in the fixation process is greatly reduced, and the phenomena of adhesion, water solubility and expansion are effectively reduced as well, thus providing conditions for large-scale industrial application of biologic active fillers and biologic active filter materials based on the polyvinyl alcohol.

Description

technical field [0001] The invention relates to the field of water treatment, in particular to a method for completing bacterial immobilization through polyvinyl alcohol-boric acid secondary crosslinking. Background technique: [0002] The traditional polyvinyl alcohol-boric acid cross-linked bacterial embedding method is a simple and low-cost immobilization method. The polyvinyl alcohol gel prepared by cross-linking by this method has high water stability, good elasticity and long service life. However, the immobilized particles have strong water absorption, and problems such as mutual adhesion, volume expansion, and water stability reduction will occur during use, especially in the environment of strong hydraulic stirring, and its expansion problem will become more obvious. The problem of adhesion and swelling of polyvinyl alcohol particles is due to the crosslinking reaction between polyvinyl alcohol and boric acid, only 2 of the 3 hydroxyl groups in boric acid participat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08
Inventor 杨宏张长利陶慕翔吴宣顾运杜强强胡希佳尚海源赵月兰王玉洁姚仁达
Owner BEIJING UNIV OF TECH
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