Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture method of microalgae with high grease content

A cultivation method and high-oil technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as failure to industrialize, and achieve the effect of increasing oil content

Active Publication Date: 2016-12-07
DALIAN OCEAN UNIV +1
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Affected by the energy crisis, the technology of using microalgae to extract biodiesel and related research have received widespread attention worldwide. Scientists have done a lot of research and experiments, but they are far from industrialization. One of the main reasons is that cost issue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isochrysis globosa 8701 was selected as the culture subject, and Isochrysis globosa 8701 was placed in an electric field with a strength of 1×10 6 Under an electric field environment of V / m for 20 minutes, then place Isochrysis globosa 8701 under a magnetic field environment with a magnetic induction intensity of 100mT for 15 minutes, and then cultivate according to the following parameters:

[0024] Glucose carbon source 4-6g / L, temperature 20±1℃, salinity 33-34, pH value 8.2-8.4, light intensity 3000-3500lux,

[0025] During the culture period, continuous aeration and continuous culture, the microalgae with the highest cell density can be obtained until the 10th day, and the highest cell density can reach 1.2 times the highest cell density of the microalgae obtained under the conditions of conventional culture methods About; cultured to the 12th day, the microalgae with the highest oil content can be obtained, and its oil content can reach 42.1%, which is 1.83 times o...

Embodiment 2

[0027] Isochrysis globosa 3011 was selected as the culture subject, and Isochrysis globosa 3011 was placed in an electric field with a strength of 2×10 6 Under the electric field environment of V / m for 10 minutes, then place Isochrysis globosa 3011 under a magnetic field environment with a magnetic induction intensity of 50mT for 20 minutes, and then cultivate according to the following parameters:

[0028] Glucose carbon source 4-6g / L, temperature 25±1℃, salinity 33-34, pH value 8.2-8.4, light intensity 3000-3500lux,

[0029] Continuous aeration and continuous culture during the culture period, the oil content can reach 41.6% until the 12th day, which is 1.81 times of the highest oil content of microalgae obtained under the conditions of conventional culture methods.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a culture method of microalgae with high grease content. The culture method is characterized by comprising the following steps that isochrysis galbana is selected as a culturing body and put in a uniform electric field environment of which the electric field intensity ranges from 1*10<6> V / m to 2*10<6> V / m for 10 min to 20 min, then the isochrysis galbana is put in a uniform magnetic field environment of which the magnetic induction intensity ranges from 50 mT to 100 mT for 15 min to 20 min, and then culture is conducted according to the following parameters that the concentration of glucose carbon source ranges from 4 g / L to 6 g / L, the temperature ranges from 19 DEG C to 26 DEG C, the salinity ranges from 33 to 34, the pH value ranges from 8.2 to 8.4 and the illumination intensity ranges from 3,000 lux to 3,500 lux; continuous aeration is conducted during the culture period, continuous culture is conducted for 10 d to 14 d, and the microalgae with the high grease content can be obtained. The culture method is low in cost, easy and convenient to operate and capable of obtaining the microalgae with the relative high grease content.

Description

technical field [0001] The invention relates to a method for increasing the oil content of microalgae with strong electric and magnetic fields, in particular to a method for cultivating microalgae with high oil content. Background technique [0002] Microalgae is a kind of autotrophic plant widely distributed on land and ocean, rich in nutrients and high in photosynthetic utilization. Its cell metabolism can produce polysaccharides, proteins, pigments, etc., making it widely used in food, medicine, genetic engineering, liquid fuel and other fields. It has a good development prospect. It uses light energy to CO 2 and H 2 O synthesizes organic substances for cell growth, and the oil in its body can not only be used as nutritional and health products, but also can be used as an important oil source for biodiesel. Affected by the energy crisis, the technology of using microalgae to extract biodiesel and related research have received widespread attention worldwide. Scientists...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/12C12P7/64C12R1/89
Inventor 胡玉才苏延明付晚涛白亚乡
Owner DALIAN OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com