Isochrysis galbana 3010 culture medium composition

The technology of a culture medium and composition, applied in the field of culture medium, can solve the problems of lack of anti-pollution ability, low growth speed and culture density, difficulty in large-scale industrial production of Chrysophylla globosa, etc., so as to be suitable for large-scale production, The effect of increasing the density of algae cells and promoting rapid growth and reproduction

Pending Publication Date: 2017-03-22
NINGBO FUTIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with Chlorella (Chlorellavulgaris) and Spirulina (Spirulina platensis), etc., Chlorella globulus lacks the characteristic medium and strong anti-pollution ability, and the growth rate and culture density are also relatively low, which is great for it. Large-scale industrial production is more difficult

Method used

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  • Isochrysis galbana 3010 culture medium composition
  • Isochrysis galbana 3010 culture medium composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0012] A golden algae 3010 medium composition, including the following components: NaNO3: 75mg / L; NaH2PO4: 5mg / L; FeCl3·6H2O: 3.16mg / L; Na2EDTA: 4.36mg / L; ZnSO4·7H2O: 0.23g / L L; MnCl2 4H2O: 0.18g / L; CuSO4 5H2O: 0.01g / L; Na2MoO4 2H2O: 0.007g / L; CoCl2 6H2O: 0.12g / L; Glucose: 2g / L; Glycine: 3g / L; Gibberellin: 1mg / L; 6-BA: 1mg / L.

[0013] Use 1000ml of filtered seawater to configure the above-mentioned composition of the golden algae 3010 culture medium, get the logarithmic growth phase of the golden algae 3010 and inoculate it into the medium for cultivation, light for 12 hours every day, and the light intensity is about 50 μmol / (m2 s), the temperature of the incubator was controlled at 25°C, and the algae cell density was detected every day.

Embodiment approach 2

[0015] A golden algae 3010 medium composition, including the following components: NaNO3: 75mg / L; NaH2PO4: 5mg / L; FeCl3·6H2O: 3.16mg / L; Na2EDTA: 4.36mg / L; ZnSO4·7H2O: 0.23g / L L; MnCl2 4H2O: 0.18g / L; CuSO4 5H2O: 0.01g / L; Na2MoO4 2H2O: 0.007g / L; CoCl2 6H2O: 0.12g / L; Glucose: 1.5g / L; Glycine: 1.5g / L L; Gibberellin: 0.5mg / L; 6-BA: 0.5mg / L.

[0016] Use 1000ml of filtered seawater to configure the above-mentioned composition of the golden algae 3010 culture medium, get the logarithmic growth phase of the golden algae 3010 and inoculate it into the medium for cultivation, light for 12 hours every day, and the light intensity is about 50 μmol / (m2 s), the temperature of the incubator was controlled at 25°C, and the algae cell density was detected every day.

Embodiment approach 3

[0018] A golden algae 3010 medium composition, including the following components: NaNO3: 75mg / L; NaH2PO4: 5mg / L; FeCl3·6H2O: 3.16mg / L; Na2EDTA: 4.36mg / L; ZnSO4·7H2O: 0.23g / L L; MnCl2 4H2O: 0.18g / L; CuSO4 5H2O: 0.01g / L; Na2MoO4 2H2O: 0.007g / L; CoCl2 6H2O: 0.12g / L; Glucose: 1g / L; Glycine: 0.5g / L ; Gibberellin: 0.1mg / L; 6-BA: 0.1mg / L.

[0019] Seawater f / 2 medium was used as the control medium, and Chrysophylla 3010 in the logarithmic growth phase was inoculated into the medium for cultivation. The light was 12 hours a day, and the light intensity was about 50 μmol / (m2·s). Controlled at 25°C, the algae cell density was detected every day.

[0020] The following table is: the cell concentration comparison table of Chrysophylla 3010 cultured in different media

[0021]

[0022] As can be seen from the experimental data in the table above, using the culture medium provided by the present invention can make Chrysophylla 3010 grow and reproduce more rapidly than using the f / 2 me...

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Abstract

The invention discloses an isochrysis galbana 3010 culture medium composition. The isochrysis galbana 3010 culture medium composition comprises the following components: 75mg/L of NaNO3, 5mg/L of NaH2PO4, 3.16mg/L of FeCl3.6H2O, 4.36mg/L of Na2EDTA, 0.23g/L of ZnSO4.7H2O, 0.18g/L of MnCl2.4H2O, 0.01g/L of CuSO4.5H2O, 0.007g/L of Na2MoO4.2H2O, 0.12g/L of CoCl2.6H2O, an additive prepared from glucose and glycine, and a plant growth regulator prepared from gibberellins and 6-BA. The isochrysis galbana 3010 culture medium composition prepared with the technical scheme is adopted for performing culture discovery on isochrysis galbana 3010; and the isochrysis galbana 3010 has the characteristics of high growth rate and high cell density.

Description

technical field [0001] The invention relates to the technical field of culture medium, in particular to a golden algae 3010 culture medium. Background technique [0002] Isochrysis galbana has the characteristics of small size, no cell wall, and easy digestion. It is rich in high-energy lipids such as polysaccharides and carotene. It is a commonly used bait microalgae in shellfish farming. Golden algae also has high medicinal value, and its β-carotene and high unsaturated fatty acids have functions of anti-aging, anti-stress and promoting nervous system development. It has important development prospects in the production of bioactive substances such as anti-aging by using algae. However, compared with Chlorella (Chlorellavulgaris) and Spirulina (Spirulina platensis), etc., Chlorella globulus lacks the characteristic medium and strong anti-pollution ability, and the growth rate and culture density are also relatively low, which is great for it. Large-scale industrial produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王兆伟江灵芝宋佳巍
Owner NINGBO FUTIAN BIOTECH
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