Runway type bioreactor applicable to sunshine factorization
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A bioreactor, track-type technology, applied in specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, microorganism-based methods, etc. It can avoid pollution, improve light utilization rate, and increase culture density.
Inactive Publication Date: 2015-06-24
OCEAN UNIV OF CHINA
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[0005] The technical problem to be solved by the present invention is to provide a runway-type bioreactor suitable for sunlight industrialization in view of the deficiencies in the prior art. Technical defects such as easy pollution, insufficient use of light, serious damage to algae during cultivation, and high cultivation costs
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[0029] (1) experimental device is prepared: two lengths are 5m, and the width is 1m oval runway pond, and experimental group is device of the present invention, and matched group is cement open runway pond;
[0030] (2) Inoculation: On the first day, the Haematococcus pluvialis liquid was inoculated in the experimental group and the control group, so that the cell inoculation density was 2.5×10 4 cells / ml; and MCM culture solution is added to the pool, the height of the culture solution is 0.15m (liquid volume 750L); the average temperature and light intensity during the culture period: 13°C at 8:00, 130μmol / m 2 / s, 25°C at 13:00, 380μmol / m 2 / s, 13°C at 17:00, 150μmol / m 2 / s;
[0031] (3) Control management: the control group uses impeller stirring to make the culture solution mix and circulate in the pool, and the rotation speed in the whole culture cycle is 30r / min, without carbon dioxid...
[0034] The method is the same as in Example 1, except that in step 2, the cell seeding density is 2×10 4 cells / ml; after 10 days of culture, the cell density of Isochrysis globosa in the experimental group reached 1.9×10 5 cells / ml, the cell density of Isochrysis globosa in the control group reached 1.1×10 5 cells / ml. Compared with the control group, the concentration of cell culture in the experimental group increased by 72.7%.
[0036] The method is the same as in Example 1, except that in step 2, the cell seeding density is 1.5×10 4 cells / ml; after 10 days of culture, the cell density of Phaeodactylum tricornutum in the experimental group reached 1.7×10 5 cells / ml, the cell density of Phaeodactylum tricornutum in the control group reached 1.1×10 5 cells / ml. Compared with the control group, the concentration of cell culture in the experimental group increased by 54.5%.
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Abstract
The invention discloses a runway type bioreactor applicable to sunshine factorization. The runway type bioreactor is used for cultivating isochrysis galbana, phacodactylum tricornutum, chaetoceros gracilis, chaetoceros mulleri and haematococcus pluvialis on a large scale and comprises a cultivating device body in the shape of a closed runway pond. A nutritive salt supplementing pipe and an injected water supplementing pipe are arranged on the side walls of the inside of the runway pond and are respectively connected with an automatic fed-batch nutritive salt supplementing device and a water treatment plant of a heat exchanger; two CO<2> gas supplementing pipes are respectively arranged on two sides of the inner bottom of the runway pond and are connected with CO<2> gas supplying devices, two screw pump mechanical propulsion devices are arranged at corners of the inside of the runway pond, a partition plate is arranged in the center of the inside of the runway pond, and buckles are respectively arranged on two outer sides of the runway pond. A stainless steel support is arranged at the outer bottom of the runway pond, and an LED lamp which is an auxiliary light source is arranged on the support; the runway pond is further provided with double-surface runway cleaning devices. The runway type bioreactor has the advantages that the illumination surface-area-to-volume ratio of algae liquid in the cultivation pond can be increased, accordingly, the illumination utilization rate of the bioreactor and the cultivation density can be increased, and the runway type bioreactor is favorable for improving the factorization micro-algae cultivation efficiency.
Description
technical field [0001] The invention relates to a bioreactor for cultivating algae, in particular to a track-type bioreactor suitable for sunlight industrialization. Large-scale cultivation of various microalgae such as Chaetoceros and Haematococcus pluvialis. Background technique [0002] Microalgae can effectively use light energy, CO 2 and inorganic salts to synthesize proteins, fats, carbohydrates and a variety of high value-added bioactive substances, which can be used to produce health food, food additives, feed, biological fertilizers, cosmetics and other natural products through microalgae cultivation. In addition, the production of gene products using algae as hosts has also attracted increasing attention in recent years. The development of microalgae biological resources is of great significance to alleviate the land, environment, energy and population crisis, to make up for the shortcomings and limitations of traditional crops, and to promote the transition from...
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