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Mixing fermentation method of chrysophyceae and photosynthetic bacteria

A technology of mixed fermentation and photosynthetic bacteria, applied in the biological field, can solve the problems of commercial algae, sale, and short storage time of microalgae

Active Publication Date: 2016-12-21
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Moreover, the liquid storage time of the existing monocultured microalgae is short and cannot be sold as commercial algae, which is also a limiting factor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for mixed fermentation of golden algae and photosynthetic bacteria, characterized in that,

[0024] Adopt Isochrysis globosa and the exothiarhodospiribacterium strains in purple sulfur photosynthetic bacteria to cultivate according to the following steps:

[0025] ⑴ Activation and cultivation of the semi-solid seeds of Rhodospira salverii: puncture the species of Rhodospira serbori into the semi-solid culture medium of Rhodospira salverii, at 30°C, light intensity: 4000 lux, light culture 8 Days, when the thread to be punctured turns red and grows a lawn, it can be the activated species of Exothiarheus sasurauerii;

[0026] ⑵ Seed culture of Rhodospira exothiasporidium: Inoculate the activated strain into the liquid culture medium of Rhodospira exothias salverii seeds, temperature 30°C, light intensity: 4000 lux, light anaerobic culture for 8 days, and test the seeds The OD650 is 1.3, and the number of viable bacteria is 650 million cfu / ml, which is the seed c...

Embodiment 2

[0035] Embodiment 2 The validity period test of mixed golden algae and photosynthetic bacteria fermentation

[0036] Test group: fermented with mixed golden algae and photosynthetic bacteria, the initial golden algae content was 41 million / ml, and the survival rate of the golden algae was detected every other month; control group: conventionally cultured golden algae, the initial golden algae content was 3.2 million cells / ml, and the survival rate was tested every month. Index determination Survival rate determination: use I-KI reagent to stain the algae to determine the living cells and dead cells, add the I-KI reagent to the algae liquid at a volume ratio of 1:1, and count under the microscope with a hemocytometer Surviving cells; dividing the viable algal cells by the initial algal cell mass is the survival rate.

[0037] Test results: As can be seen from Table 1, the survival rate of Chrysophylla in the mixed fermentation can reach 51.2% after half a year, while the survi...

Embodiment 3

[0040] Example 3 Mixed fermented golden algae and photosynthetic bacteria in the production test of water purification agent and auxiliary bait for seedling cultivation of Argopecton irradians larvae and juvenile shellfish

[0041] From February 8th to March 20th, 2015, at a seawater nursery farm in Qindou Town, Zhanjiang City, 80m away 3 In the water sports nursery pool, the daily application rate is 1L of mixed fermentation broth of golden algae and photosynthetic bacteria;

[0042] Test results: The growth rate of planktonic larvae increased by 38.4%, the survival rate of the planktonic stage increased by 60.3%, eye spots appeared 40 hours earlier, the metamorphosis level of spat increased by 24.5%, and the yield increased by 195%, achieving remarkable results.

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PUM

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Abstract

The invention provides a mixing fermentation method of chrysophyceae and photosynthetic bacteria, which belongs to the field of biotechnology. The invention concretely provides the mixing fermentation method of isochrysis galbana and ectothiorhodospira shaposhnikovii, isochrysis galbana and ectothiorhodospira shaposhnikovii are respectively subjected to seed activation, culture, and then mixing fermentation in an illuminating fermentation tank, so that isochrysis galbana content is greatly increased which is 5-10 times of that through normal culture.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular provides a method for mixed fermentation of Chrysophytes and photosynthetic bacteria, in particular a method for mixed fermentation of Isochrysis globosa and Exothorhodospiri. Background technique [0002] Isochrysis globosa belongs to Chrysophyta, Purinellaceae, Isochrysophyceae, Isodinophyceae. It has the characteristics of thin cell membrane, no cell wall, high protein content, and easy digestion and absorption by fish, shrimp, crab and shellfish. It is widely used in many microalgae in the production of aquatic economic animals. Chrysophylla is small in size, has no cell wall, thin cell membrane, It is easy to digest, high in oil and polyunsaturated fatty acid, and has become a kind of economical algae with high research value. However, Chrysophytes have strict requirements on temperature, light intensity and nutrient factors, and are easily polluted by various microorganisms s...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/12C12R1/89C12R1/01
CPCC12N1/12C12N1/20
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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