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A technology of Dunaliella salina and its cultivation method, which is applied in the field of rapid cultivation of Dunaliella salina, and can solve the problems of slow growth, poor quality, easy pollution, etc.
Inactive Publication Date: 2015-04-01
LINYI UNIVERSITY
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[0003] Traditionally, Dunaliella salina is cultivated in an open runway cement tank and using f / 2 culture solution formula, which has slow growth, easy pollution, low yield, low β-carotene content, poor quality, and low benefit
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[0016] The preparation of step 1 culture medium takes a 3000 ml Erlenmeyer flask, puts into 2000 ml of boiled and cooled seawater, adds 5 grams of ammonium nitrate, 3 grams of urea, 1.5 grams of potassium dihydrogen phosphate, 18 grams of sodium bicarbonate, iron citrate 0.2 g, vitamin B 1 0.02 mg, vitamin B 12 0.1 mg, 0.01 mg of 920 phytostimulant, 0.01 g of EDTA sodium salt, sealed with plastic wrap, shake well on a shaker for 15 minutes, and save for later use;
[0017] Step 2 Inoculation Take a 1000 ml Erlenmeyer flask, add 500 ml of culture solution prepared in step 1, inoculate with Dunaliella salina algae that are in the logarithmic growth phase, grow vigorously, and have no pollution. The density after inoculation reaches 2.5 ×10 4 cell / ml, sealed with newspaper and rubber band after autoclaving;
[0018] Step 3 Lighting incubator cultivation Put the Erlenmeyer flask inoculated in step 2 into a light incubator for cultivation. For the first 5 days of cultivation, ...
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Abstract
The invention discloses a fast culture method for dunaliella tertiolecta, belonging to the field of aquaculture. Conventionally, dunaliella tertiolecta which is cultured by using an open track cement pool and a f / 2 culture solution formula is slow to grow, easy to pollute, low in yield, low in content of beta-carotene, poor in quality and low in efficiency. In order to overcome defects of the conventional culture method, the dunaliella tertiolecta is cultured by using an illumination incubator and nutrients such as ammonium nitrate, urea, monopotassium phosphate, sodium hydrogen carbonate, ferric citrate, vitamin B1, vitamin B12, 920 plant growth stimulin and the like are further added into the nutrient solution, nutritive salt is supplemented in the later stage of culture, the illumination intensity and temperature are enhanced, and continuous growth of algae cells is promoted and beta-carotene is stressed to be accumulated, so that the dunaliella tertiolecta is fast to grow, high in yield, hard to pollute, good in quality, simple and convenient to operate, less in investment and high in efficiency.
Description
technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a rapid cultivation method for Dunaliella salina. Background technique [0002] Dunaliella salina is a diflagellated single-celled green alga that grows in inland salt lakes and can live in ordinary seawater after domestication. It belongs to Chlorophyta, Chlorophyta, Volvox, Salinaceae, Salina. The cells are oval, oval or pear-shaped, 18-28 μm long and 9.5-14 μm wide, and their morphology changes greatly under different salinity, light and temperature. The front end of the cell is concave, and there are two flagella of equal length in the depression, and the flagella is about 1 / 3 longer than the cell; there is a red eye point at the base of the flagella. There is a cup-shaped pigment body in the body, mainly chlorophyll and carotenoids (mainly β-carotene). Asexual reproduction, the algal body splits into two during the movement, each forming a new body. Cells are rich ...
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