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Ultrasonic extraction method for beta-carotene in Dunaliella salina powder

A technology of Dunaliella salina powder and carotene, which is applied in the field of food science, can solve the problems of affecting extraction and high extraction cost, and achieve the effects of easy recovery, single extraction solvent, and high safety

Inactive Publication Date: 2015-11-04
中山鼎晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, organic solvent extraction and CO2 supercritical extraction are the most common methods for extracting β-carotene. The former is determined by the characteristics of β-carotene itself being soluble in organic solvents. The operation is simple and the extraction is convenient, but it may affect Extraction of Other Physiologically Active Substances from Salina
The latter has the dual functions of extraction and separation, with high extraction efficiency and no residual organic solvent, but the extraction cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for ultrasonically extracting β-carotene in Dunaliella salina powder, comprising the following steps:

[0028] (1) Weigh 0.5g of Dunaliella salina powder, add appropriate amount of water to cool down to -40°C, keep it for 2 hours after cooling down, and thaw by microwave with 400W power until there are no ice crystals;

[0029] (2) Saponification: Take the thawed sample in step (1) and put it into a 100ml conical flask with a stopper, add a saponification agent 15 times the volume of the sample, heat it under reflux at 25°C for 1.5h, and then put the conical flask in the refrigerator Cool rapidly in the freezer, centrifuge the sample solution in the cooled Erlenmeyer flask at a speed of 4000r / min for 15min, remove the supernatant, and obtain the saponified sample solution;

[0030] (3) Put the saponified sample solution obtained in step (2) into a stoppered Erlenmeyer flask, add ethyl acetate, place it in an ultrasonic generator at 20°C, perform ultrasonic extr...

Embodiment 2

[0034] A method for ultrasonically extracting β-carotene in Dunaliella salina powder, comprising the following steps:

[0035] (1) Weigh 5g of Dunaliella salina powder, add appropriate amount of water to cool down to -10°C, keep it for 3 hours after cooling down, and thaw by microwave with 450W power until there are no ice crystals;

[0036] (2) Saponification: Take the thawed sample in step (1) and put it into a 100ml Erlenmeyer flask with stopper, add saponification agent 20 times the volume of the sample, heat it under reflux at 35°C for 2 hours, then place the Erlenmeyer flask in the refrigerator to freeze Cool rapidly in the cabinet, centrifuge the sample solution in the cooled Erlenmeyer flask at a speed of 5000r / min for 30min, remove the supernatant, and obtain the saponified sample solution;

[0037] (3) Put the saponified sample solution prepared in step (2) into a stoppered Erlenmeyer flask, add 90% ethanol aqueous solution, place it in an ultrasonic generator, extra...

Embodiment 3

[0041] A method for ultrasonically extracting β-carotene in Dunaliella salina powder, comprising the following steps:

[0042] (1) Weigh 2g of Dunaliella salina powder, add some water to cool down to -20°C, keep it for 2.5 hours after cooling down, and then heat and thaw with microwave at 420W power until there are no ice crystals;

[0043] (2) Saponification: Take the thawed sample in step (1) and put it into a 100ml Erlenmeyer flask with a stopper, add a saponification agent 18 times the volume of the sample, heat it under reflux at 30°C for 1.8h, and then place the Erlenmeyer flask in the refrigerator Cool rapidly in the freezer, centrifuge the sample solution in the cooled Erlenmeyer flask at a speed of 4500r / min for 20min, remove the supernatant, and obtain the saponified sample solution;

[0044] (3) Put the saponified sample solution obtained in step (2) into a stoppered Erlenmeyer flask, add 95% ethanol aqueous solution, place it in an ultrasonic generator and perform ...

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Abstract

The present invention relates to an ultrasonic extraction method for beta-carotene in Dunaliella salina powder. The ultrasonic extraction method comprises: adding water to Dunaliella salina powder and cooling, carrying out microwave heating thawing, sequentially carrying out saponification and cooling centrifugation, removing the supernatant, adding an extraction solvent to the sample solution, carrying out ultrasonic extraction, and carrying out cooling crystallization to obtain the beta-carotene extract. According to the present invention, the sample is subjected to freezing-microwave thawing, such that the water content in the sample can be reduced, and the subsequent operation is easy; the saponification is performed on the sample before the extracting, such that the beta-carotene can be completely extracted; the ethyl acetate or 90-95% ethanol aqueous solution is adopted as the ultrasonic extraction solvent and the extraction is performed for about 0.5-0.7 h at the temperature of 25 DEG C, such that the beta-carotene is substantially and completely extracted; and the extraction solvent single, is easy to recover, and has high safety, and the method can be widely used in massive food or medicine and other fields.

Description

technical field [0001] The invention belongs to the technical field of food science, and in particular relates to an ultrasonic extraction method for beta-carotene in Dunaliella salina powder. Background technique [0002] Salina is a kind of halophilic algae with tiny shape, no cell wall and bare protoplasm. It belongs to Chlorophyta, Chlorophyta, Volvox, Salinaceae, and Salina genus Dunaliella. Salina belongs to high-salt adversity organisms. It is one of the most salt-tolerant eukaryotes discovered so far. It mostly lives in natural salt ponds or salt lakes. The special growth environment has achieved its special growth mechanism, and its adaptation to the environment Strong sex, fast growth and reproduction. Salina is rich in polysaccharides, trace elements, proteins, highly unsaturated fatty acids and other nutrients. [0003] The molecular formula of β-carotene is C 40 H 56 and the relative molecular weight is 536.88. It is composed of four isoprene double bonds co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
Inventor 郭狄
Owner 中山鼎晟生物科技有限公司
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