Method for dewatering microalgae with bubble column

A technology of bubbles and microalgae, which can be used in sterilization methods, methods of supporting/immobilizing microorganisms, methods of sampling biological materials, etc., and can solve problems such as increasing costs

Inactive Publication Date: 2000-01-12
EASTMAN CHEM CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] The use of chemical additives such as flocculants and coagulants has limited the commercial exploitation of Dunaliella salina as a sourc...

Method used

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  • Method for dewatering microalgae with bubble column
  • Method for dewatering microalgae with bubble column
  • Method for dewatering microalgae with bubble column

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 8-10

40CFH

1500

84

[0295] 2000 milliliters of brines containing low, medium and high concentrations of Dunaliella salina were respectively charged into the flotation cell and mixed for 10 minutes. The effect of cell concentration on carotenoid recovery is summarized in Table 7.

[0296] Example number

Carotenoid concentration

Carotenoids

Recovery rate(%)

26

0.00032 mg / ml saline (~1800 cells

/ ml)

90％

27

0.002 mg / ml saline

70％

28

0.125 mg / ml saline (~1.3M cells /

ml)

66％

[0297] 2000 milliliters of suspended brine containing Dunaliella salina is loaded into the flotation cell. Triton X-100 was added to the brine and the solution was mixed for 1 minute at 1500 rpm without air. Then the air valve was opened and the suspension was mixed with air for 20 minutes. Carotenoid recovery was measured after 10 minutes and the results are summarize...

Embodiment 34

[0302] The foam generated in the test described in Example 34 was collected and treated with a Jameson tank having the geometry described in Example 34 to further concentrate the carotenoids. Groove J g The value is 0.27 cm / s. The jet velocity was 10.6 m / s. The surface velocity of the downcomer is 0.13 m / s. The downcomer liquid residence time was 23.2 seconds. The ratio of air to feedstock was 0.49. The recovery of carotenoids from this process averaged 89.7%. The percentage of solids in the foam was 0.5% (excluding gases). Example 36

[0303] The brine containing the Dunaliella salina suspension was treated with a Jameson tank having the geometry described in Example 34 without any mechanical or chemical pretreatment. Groove J g The value is 0.65 cm / s. The jet velocity was 46.1 m / s. The surface velocity of the downcomer is 0.175 m / s. The downcomer liquid residence time was 17.5 seconds. The ratio of air to feedstock was 0.88. The recovery of carotenoids from this...

Embodiment 37

[0304] The foam generated in the test described in Example 34 was collected and treated with a Jameson tank having the geometry described in Example 34 to further concentrate the carotenoids. The Jg value of the groove is 0.29 cm / sec. The jet velocity was 11.9 m / s. The surface velocity of the downcomer is 0.14 m / s. The downcomer liquid residence time was 21.7 seconds. Feed pressure was 22 psi. The ratio of air to feedstock was 0.49. Carotenoid recovery from this process averaged 68%. The percent solids in the foam was 8.3% (excluding gas). Example 38

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Abstract

A process for dewatering an aqueous suspension of microalgae is disclosed in which the aqueous suspension of the algae is introduced into a bubble column or a modified bubble column for generating a froth of bubbles and adsorbed algal cells that can be separated from the aqueous suspension. In one advantageous embodiment, the bubble column is a multi-stage loop-flow flotation column that has three loop-flow zones, each of which is defined by a draft tube concentrically mounted in the column to divide each loop-flow zone into a riser and the downcomer. Fine bubbles of gas and brine are in cocurrent upward flow in the riser and in cocurrent downward flow the downcomer. A higher gas holdup is promoted in the riser than in the downcomer, thereby circulating the brine in loop-flow upwardly through the riser and downwardly through the downcomer. Liquid communication between adjacent loop-flow zones is substantially eliminated. A froth enriched in algae is generated that can be separated from the aqueous suspension. The process has application in the recovery of mixed carotenoids from Dunaliella salina.

Description

field of invention [0001] The present invention relates to a method for extracting substances from algae. More specifically, the present invention relates to a method for extracting mixed carotenoids from Dunaliella salina. Background of the invention [0002] Carotenoids are deep orange-yellow pigments found in citrus and yellow vegetables, as well as in many dark green foods. Beta-carotene is the most abundant of the various carotenoids. Beta-carotene can be converted into vitamin A in the body. Vitamin A is a fat-soluble vitamin. Unlike water-soluble vitamins that cannot be stored in the body, it can be stored in the body, mainly in the liver, for a period of time. If you take too much vitamin A, it is harmful to the human body. However, beta-carotene is converted to vitamin A in the body as needed and is generally considered a non-toxic source of vitamin A, even when taken in large amounts. [0003] Beta-carotene has been shown to act as an antioxidant that protects...

Claims

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Application Information

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IPC IPC(8): B03D1/02C12M1/12C12M1/33C12N1/02C12N1/06C12N1/12C12P23/00
CPCC12M47/06C12M29/06C12M47/02C12M33/14
Inventor S・A・格尔彻尔J・S・卡内尔
Owner EASTMAN CHEM CO
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