Method for cultivating green Dunaliella salina of liking for salt extremely

A cultivation method, the technology of Dunaliella, applied in the direction of single-cell algae, etc., can solve the problems that do not involve the cultivation technology of extremely halophilic green Dunaliella

Inactive Publication Date: 2003-10-08
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After retrieval, the current research on Dunaliella salina (Dunaliella.salina) mainly involves: analysis of genetic diversity among halophilic species of Dunaliella genus, culture technology and application of Dunaliella salina, physiological response of Dunaliella to salinity, etc. , none of which involve the cultivation technology of the extreme halophilic green Dunaliella (Dunaliella.viridis)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of culture medium: buy 100 liters of brine with a salinity of 15°Be’ from the high-grade brine production area of ​​the Changlu salt area salt field, first use absorbent cotton to filter out the dirt; then add the nutrient salt NaH 2 PO 4 1 g and urea ie CH 4 N 2 O 11 grams; then test the pH value, if the pH value is in the range of 6 to 8, no adjustment is required, if it exceeds this range, use dilute 10% HCl or 10% NaOH to adjust the pH value to 6 to 8; Chlorine Oxide Disinfectant (CLO 2 content is 2%) 5 grams of brine is sterilized and algae-removed to obtain a culture solution. Inoculate after 48 hours.

[0024] 2. Preparation of algae species: 1 liter of brine with a salinity of 25 ° Be' was taken from the high-grade brine production area of ​​the Changlu Salt Area Salt Field, Bohai Bay, China. The first is pre-cultivation. Add 1 gram of Artemia dry powder to the brine, and then use a conical flask to culture in a light incubator. The culture c...

Embodiment 2

[0029] 1. Preparation of culture medium: buy 100 liters of brine with a salinity of 18°Be’ from the high-grade brine production area of ​​the Changlu salt area salt field, first use absorbent cotton to filter out the dirt; then add the nutrient salt NaH 2 PO 4 1 g and urea ie CH 4 N 2 O 11 grams; then test the pH value, if the pH value is in the range of 6 to 8, no adjustment is required, if it exceeds this range, use dilute 10% HCl or 10% NaOH to adjust the pH value to 6 to 8; Chlorine Oxide Disinfectant (CLO 2 content is 2%) 5 grams of brine is sterilized and algae-removed to obtain a culture solution. Inoculate after 48 hours.

[0030] 2. Preparation of algae seeds: 1 liter of brine with a salinity of 26% was taken from the high-grade brine production area of ​​the Changlu Salt Area Salt Field in the tidal flat of Bohai Bay, China. The first is pre-cultivation. Add 0.8 grams of Artemia dry powder to the brine, and then use a conical flask to culture in a light incubat...

Embodiment 3

[0035] 1. Preparation of culture medium: buy 100 liters of brine with a salinity of 22°Be’ from the high-grade brine production area of ​​the Changlu salt area salt field, first use absorbent cotton to filter out the dirt; then add the nutrient salt NaH 2 PO 4 1 g and urea ie CH 4 N 2 O 11 grams; then test the pH value, if the pH value is in the range of 6 to 8, no adjustment is required, if it exceeds this range, use dilute 10% HCl or 10% NaOH to adjust the pH value to 6 to 8; Chlorine Oxide Disinfectant (CLO 2 content is 2%) 5 grams of brine is sterilized and algae-removed to obtain a culture solution. Inoculate after 48 hours.

[0036] 2. Preparation of algae species: 1 liter of brine with a salinity of 26 ° Be' was taken from the high-grade brine production area of ​​the Changlu Salt Area Salt Field, Bohai Bay, China. The first is pre-cultivation. Add 1.2 grams of Artemia dry powder to the brine, and then use a conical flask to culture in a light incubator. The cultu...

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PUM

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Abstract

A process for culturing the extremely halophilous green Du's algae used for health-care, medicinal and gene engineering purposes includes preparing culture liquid, regulating sanility and pH value, disinfecting, removing unuse algae, preparing alga seeds, inoculating, and controlling the environment of large-area culturing.

Description

technical field [0001] The invention relates to a method for cultivating algae, in particular to a method for cultivating Dunaliella. Background technique [0002] Dunaliella viridis growing in extreme halophilic brine environment (salinity greater than 15°Be') has unique biological characteristics, and the algal body contains a variety of biologically active substances. It can be applied in the fields of human health care, biopharmaceuticals, genetic engineering, etc., to develop natural health care products or biological medicines, and to study salt-tolerant genes. It has certain application value and broad application prospects. The current research on Dunaliella salina (Dunaliella.salina) mainly involves: analysis of genetic diversity among halophilic species of Dunaliella genus, culture technology and application of Dunaliella salina, physiological response of Dunaliella to salinity, etc. , all do not involve the research on the culture techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12
Inventor 张福马若欣
Owner TIANJIN UNIV OF SCI & TECH
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