Method capable of promoting stripped shellfish eggs to be mature in vitro

A technology for eggs and shellfish, applied in the field of in vitro maturation of stripped shellfish eggs, which can solve problems such as egg damage, low fertilization rate, hatching rate, and embryo deformity

Inactive Publication Date: 2010-01-20
OCEAN UNIV OF CHINA
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] At present, ammonia water is mainly used to promote maturation of eggs in vitro. However, although ammonia water can rupture all egg blastocysts, the damage to eggs is serious. Therefore, the fertilization rate and hatching rate are relatively low, and the embryos are seriously deformed.

Method used

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Embodiment Construction

[0007] In the present invention, the clam scallop that cannot be fertilized by normal stripping is taken as an example. The clam shells are taken, the sperm and eggs are obtained by stripping, the eggs are filtered, and the eggs are washed, and then the treated sperm and eggs are collected. In the barrel, first treat the eggs with 0.005%-0.01% volume percent ammonia water for 10-15 minutes, then wash the eggs with 0.001M-0.002M 5-hydroxytryptamine for 30-45 minutes, then wash the eggs. Eggs are combined for artificial insemination, the fertilization rate can reach more than 40%, and the hatching rate of fertilized eggs is 50%-70%.

[0008] The above-mentioned embodiment is also applicable to other spawning marine shellfish such as abalone, clam, razor clam, clam, pearl oyster or mussel, which cannot be fertilized by stripping eggs, and similar effects can be obtained.

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Abstract

A method capable of promoting stripped shellfish eggs to be mature in vitro is characterized by first stripping off mature shellfish to obtain eggs and sperm, filtering and washing the eggs after stripping, then washing the eggs after being treated by ammonia water at a concentration of 0.005-0.01% for 10-15min and then by 5-hydroxytryptamine at a concentration of 0.001-0.002M for 30-45min, finishing artificial fertilization after combination of the eggs and sperm, which are promoted to be mature in vitro and enabling fertilized eggs to hatch and grow to normal D-type larvae. The shellfish is abalone, bloody clams, sinonovacula constricta, clams, pearl shells or mussels. In the method, the complementary actions of the ammonia water and 5-hydroxytryptamine are adopted, therefore, the respective side effects of the ammonia water and 5-hydroxytryptamine are reduced, the fertilization rate and hatchability are improved and the deformity is less. The method is simple in operation and can be applied to seedling production.

Description

technical field [0001] The invention relates to a method for in vitro maturation of shellfish ovum, in particular to a method for in vitro maturation of stripped shellfish ovum. Background technique [0002] For shellfish undergoing in vitro fertilization, there are two timings for the sperm to enter the egg. One is that the primary oocyte is before the first meiotic division, and there is a blastocyst, and the blastocyst disappears after the sperm enters, such as Oyster oyster near the river, etc.; One is that the primary oocyte is in the metaphase of the first meiosis, and the blastocyst has disappeared before fertilization is possible, such as Chlamys farreri, Hepu pearl oyster, mussel, wrinkled abalone, etc. period, the meiosis of the oocyte stagnates in the first meiotic prophase, that is, the prophase arrest occurs, so the eggs obtained by stripping are generally not fertilizable. This just needs to carry out certain processing to the peeled shellfish ovum, make it fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王昭萍邸炜鹏于瑞海
Owner OCEAN UNIV OF CHINA
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