Method for improving hydrogen production of chlamydomonas

A technology of Chlamydomonas and Rhinoceros is applied in the field of microorganisms, which can solve problems such as high cost, and achieve the effect of solving contradictions and broadening the scope of symbiotic objects.

Active Publication Date: 2017-03-22
SHANGHAI NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the production of hydrogen mainly includes two ways of hydrogen production from

Method used

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  • Method for improving hydrogen production of chlamydomonas
  • Method for improving hydrogen production of chlamydomonas
  • Method for improving hydrogen production of chlamydomonas

Examples

Experimental program
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Embodiment 1

[0081] The hydrogen production cultivation of embodiment 1 algal bacteria

[0082] Chlamydomonas was cultured to saturation phase (OD 750 About 3.0), centrifuged at 5000rpm for 5 minutes with a centrifuge, removed the supernatant, added an appropriate amount of sulfur-deficient medium to resuspend 3 times, to completely remove the sulfur in the original medium. The Chlamydomonas treated with sulfur deficiency was transferred to a 60ml hydrogen-producing culture bottle according to the chlorophyll concentration of 0.5mg / L, and the volume was adjusted to 45ml with sulfur-deficiency medium. The nitrogen-fixing bacteria cultured to the logarithmic phase (OD 600 =1) According to the volume ratio of bacteria and algae 1:80, 1:40, 3:80, 1:20 respectively into the hydrogen-producing bottle that has been added with Chlamydomonas, and finally seal the bottle mouth with a rubber stopper. The culture system with only Chlamydomonas and nitrogen-fixing bacteria was used as the control g...

Embodiment 2

[0099] Example 2 Contribution of Chlamydomonas reinhardtii and nitrogen-fixing bacteria to hydrogen production in co-culture system

[0100] In order to understand the contribution of algae and bacteria to the improvement of hydrogen production in the mixed co-culture system of Chlamydomonas and nitrogen-fixing bacteria, the pure Chlamydomonas culture system, the pure nitrogen-fixing bacteria culture system, and the Chlamydomonas-diazotobacter co-cultivation system under the optimal hydrogen production conditions were combined. The culture system and optimal mixing ratio of Chlamydomonas lysate (that is, algae treated with liquid nitrogen freeze-thawing)-diazotobacter co-culture system (total of 4 different culture systems) were continuously measured for hydrogen after 24 hours of darkness. The results are as follows Figure 5 shown.

[0101] It can be seen from the results that the pure nitrogen-fixing bacteria culture system and the Chlamydomonas lysate-diazotobacter co-cu...

Embodiment 3

[0105] Growth status and distribution pattern of Chlamydomonas and nitrogen-fixing bacteria in the co-cultivation system of embodiment 3 algae

[0106] 1. In order to detect the growth of Chlamydomonas after co-cultivation with nitrogen-fixing bacteria, the co-cultivation conditions with the best hydrogen production were detected in the normal TAP medium and the hydrogen-producing TAP-S medium respectively. The growth of the following two algae.

[0107] The co-cultivation operation of algal bacteria under normal culture conditions is as follows: culture nitrogen-fixing bacteria in logarithmic phase (OD600 is about 1.0), centrifuge at 5000rpm for 5 minutes, remove the supernatant, and resuspend three times with the same volume of TAP to completely remove nitrogen-fixing bacteria For the culture medium, the resuspended nitrogen-fixing bacteria are mixed with Chlamydomonas according to the ratio of bacteria and algae under the optimal hydrogen production conditions, and added ...

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Abstract

The invention discloses a method for improving hydrogen production of chlamydomonas. The method includes steps of commonly cultivating chlamydomonas and nitrogen-fixing bacteria; before commonly cultivating, cultivating chlamydomonas to a saturated period and cultivating nitrogen-fixing bacteria to a logarithmic phase, wherein the nitrogen-fixing bacteria is round brown nitrogen-fixing bacteria; the chlamydomonas is Chlamydomonas reinhardtii cc849 (with cell wall type) and Chlamydomonas reinhardtii cc124 (cell wall defective type). The method can effectively improve the hydrogen production of chlamydomonas; in relative to the hydrogen production of independent chlamydomonas, the maximum hydrogen production can be improved by 16 times.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for increasing the hydrogen production of Chlamydomonas. Background technique [0002] Energy is the material basis for human survival and development. The energy used by human beings is mainly fossil fuels such as oil, natural gas and coal. Fossil fuels are non-renewable energy sources with limited reserves; the combustion products of fossil fuels are CO 2 It will cause the greenhouse effect, and the combustion by-products of nitrogen oxides and sulfur oxides can cause air pollution. [0003] Hydrogen is a relatively good energy carrier, with significant advantages such as high efficiency, no pollution, and wide application range. At present, the production of hydrogen mainly includes two ways of hydrogen production from fossil raw materials and hydrogen production by electrolysis of water, which are expensive. The former needs to consume a large amount of precious non-...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P3/00C12R1/89C12R1/065
CPCC12P3/00C12P39/00
Inventor 许丽丽程祥龙李燕均王全喜
Owner SHANGHAI NORMAL UNIVERSITY
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