Method of increasing hydrogen releasing efficient of chlamydomonas

A Chlamydomonas high-efficiency technology, applied in the field of biological hydrogen production, can solve the problems of limited host cell types, reduced protons, and inability to multiply, and achieve the effect of improving the hydrogen release efficiency of Chlamydomonas

Inactive Publication Date: 2005-08-24
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is to use physiological and biochemical methods to improve the efficiency of hydrogen desorption, such as synchronous cultivation of Chlamydomonas, changes in the content of acetic acid in the Chlamydomonas culture medium, and adjustment of the pH value of the Chlamydomonas culture medium. Improved, but not multiplied [6]
Another way is to use the means of molecular biology to try to improve the hydrogen desorption efficiency [7][8][9] , to screen oxygen-resistant Chlamydomonas mutants, change the binding site of hydrogenase and oxygen, theoretically the production can be doubled, but, because the gene information capacity in a single cell is limited, adding foreign genes is very important for cell proliferation and metabolic system Undoubtedly, it is a burden, so the number and size of genes that can be introduced into a single cell are limited; and when using genetically recombined cells, another bottleneck problem encountered is that the types of host cells that can be used are extremely limited, because many In some cases, the product not only stays in the cell but also has no activity, which brings great trouble to the subsequent separation process and is unstable after subculture
[0004] The Melis two-step dehydrogenation method completely relies on the respiration of Chlamydomonas to consume the oxygen in the solution. The time is relatively long, the growth state of Chlamydomonas is further affected, and the electrons generated by photosynthetic hydrolysis are not much left, which affects the hydrogen desorption rate. At the same time, Chlamydomonas is still consuming glacial acetic acid after closed culture, the pH value of the culture solution continues to rise, and the protons that the solution can provide are relatively reduced, which also affects the hydrogen release rate.
In addition, under the stress conditions of lack of sulfur and closed culture, the growth state of Chlamydomonas significantly decreases, which affects the ability of the entire hydrogen release.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0014] Example 1 Algae and bacteria symbiotic hydrogen desorption method

[0015] 1. Experimental materials and instruments: Chlamydomonas Chiamy reinhardtii cc125mt+, wild type, purchased from the Chlamydomonas Center of Duke University, USA.

[0016] Culture method: Tris-acetate-phosphate medium (TAP) [11]

[0017] NH 4 Cl 0.4g / L

[0018] MgSO 4 ·7H 2 O 0.1g / L

[0019] CaCl 2 0.0377g / L

[0020] K 2 HPO 4 0.108g / L,

[0021] K H 2 PO 4 0.056g / L

[0022] Tris 2.42g / L

[0023] Glacial acetic acid 1ml

[0024] Trace elements 1ml

[0025] The medium of TAP-S is to contain SO 4 2- The salts are all with the corresponding Cl - Salt replacement, including sulphate in trace elements.

[0026] ST-04 trace water chromatograph (Beijing Analytical Instrument Factory), thermal conductivity detector, N 2 As carrier gas, determined by external standard method, chromatographic workstation JF-9902 (Beijing Analytical Instrument Factory)

...

Embodiment 2

[0036] Example 2 Co-cultivation of Chlamydomonas and immobilized bacteria

[0037] 1. Experimental drugs

[0038] 4% sodium alginate, sterilized at 4°C, 0.05mol / L CaCl 2

[0039] 2. Bacteria immobilization method

[0040] 1). Collect the bacteria by centrifugation at 1000g, wash twice with sterile water, and use the OD of the bacteria solution 600 between 0.5000-0.6000;

[0041] 2). Add the obtained bacteria paste to 1 / 3 volume of TAP-S medium and mix well;

[0042] 3). Add 2 times the volume of 4% sodium alginate and mix well;

[0043] 4). Add 0.05mol / l CaCl 2 Incubate in a water bath at 37°C for 10 minutes;

[0044] 5). Put the mixture of sodium alginate and bacteria paste into the syringe, and use a No. 9 needle to drop CaCl 2 in solution;

[0045] 6). Pour off the solution and rinse once with sterile deionized water;

[0046] 7). Re-add 00.05mol / L CaCl 2 Solution, equilibrate at 4°C for 6 hours;

[0047] 8). Pour off the CaCl 2 solution, washed twice with TAP-...

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Abstract

A process for increasing the hydrogen releasing efficiency of chlamydomonas features use of the dominant complementation between phycobionts.

Description

technical field [0001] The invention relates to the field of biological hydrogen production, and more specifically, the invention relates to a method for improving the hydrogen release efficiency of Chlamydomonas. Background technique [0002] Micro-green algae reproduce quickly, distribute widely, and are easy to breed. They automatically collect light energy, accumulate energy spontaneously, and convert it quickly. Therefore, hydrogen production from green algae is the most economical, convenient, and easy-to-implement technology in the future. In 1998, the International Energy Agency The evaluation report believes that the photolysis hydrogen production method of green algae reversible hydrogen production enzyme is the most promising research direction. The United States, Germany, Japan and other countries have invested heavily in supporting biological hydrogen production, hoping to enter industrial production as soon as possible and break through the bottleneck of energy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B3/02C12N1/00C12N1/12
Inventor 朱毅李永兴王可玢黄辉马晶晶白克智李良璧匡廷云
Owner INST OF BOTANY CHINESE ACAD OF SCI
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