Hyaluronic acid-containing pet molar rod composition as well as preparation method and application thereof

Abstract

The present invention discloses a hyaluronic acid-containing pet molar rod composition as well as a preparation method and applications thereof. The hyaluronic acid-containing pet molar rod composition comprises meat powder and hyaluronic acid containing thallus crushing substances or salts thereof; the composition uses viscosity of hyaluronic acid and thallus crushing substances to replace starch, gel and other adhesives or forming agents, and after the molar rod is prepared, due to rich hyaluronic acid, the molar rod can inhibit bacteria, diminish inflammation, remove halitosis, protect gingiva and oral mucosa and also has the health-care effects of brightening hair and strengthening bones after being digested and absorbed, the unicity of the function of a traditional molar rod is improved, thalli are not removed in the preparation process, cost is saved, resources are fully utilized, and the molar rod has better nutrition and palatability; the effect of an adhesive can be achieved;and more importantly, the crushed thallus can form relatively hard particles after being dried, so that residues, tartar or dental plaque on the teeth of the pet can be effectively removed.

Description

technical field

[0001] The invention relates to the technical field of pet food, in particular to a pet molar stick composition containing hyaluronic acid, a preparation method and an application thereof. Background technique

[0002] With the continuous improvement of the living standards of Chinese residents and the reduction of family size, keeping pets has become a part of the life of more and more urbanites. People's growing love for pets has led to a rapid increase in the number of pets, which in turn has prompted the gradual growth of the pet food industry. In pet food, the demand for molar sticks is increasing day by day. Teething sticks can avoid or reduce pet damage to furniture. More importantly, molar sticks can help remove food residues on pet teeth, control the accumulation of tartar, and remove pets. Dental plaque, keeps gums healthy, helps eliminate bad breath in pet dogs, maintains white teeth, and reduces potential dental serious risks.

[0003] The patent...

Examples

preparation example Construction

[0053] The invention provides a preparation method of a pet molar stick composition, which comprises the following steps:

[0054] Adding hyaluronidase, acid or alkali to the hyaluronic acid fermentation broth containing bacteria used to ferment and produce hyaluronic acid to degrade to obtain a degradation solution;

[0055] Precipitate the degradation solution to obtain a precipitate, and dry the precipitate to obtain hyaluronic acid or its salt containing broken bacteria;

[0056] The pet molar stick composition is obtained by mixing the hyaluronic acid or its salt containing broken bacteria and meat powder.

[0057] In a preferred embodiment of the present invention, wherein the hyaluronidase is selected from hyaluronidase derived from bovine testis, hyaluronidase derived from Mianyang testis, hyaluronidase derived from microorganisms, recombinant One or more of human hyaluronidase and chondroitinase ABC;

[0058] The microorganism-derived hyaluronidase may be, for examp...

Embodiment 1

[0086] (1) Take 10L of hyaluronic acid fermentation broth containing bacteria for fermentation and production of hyaluronic acid, with an initial kinematic viscosity of 50000mm 2 / s, add 300 μL of hyaluronidase (enzyme activity 6000IU / mL) under stirring, hydrolyze in a water bath at 37°C for 2h, heat to 60°C to inactivate for 0.5h, and the final kinematic viscosity of the degradation solution is 138mm 2 / s, the measured molecular weight of hyaluronic acid is 400kDa.

[0087] (2) Add 30L ethanol to the degradation solution until the volume concentration is 70% to produce a precipitate, stir for 0.5h, let stand for 0.5h, pour off the supernatant, dehydrate the precipitate with ethanol to a volume concentration of 90%, and place the precipitate after dehydration Oven-dried at 50°C until the water content is about 18%, put it into a pulverizer and pulverize it into powder to obtain hyaluronic acid powder containing broken bacteria, wherein the hyaluronic acid or its salt containin...

Embodiment 2

[0091] (1) Take 1L of hyaluronic acid fermentation broth containing bacteria for fermentation and production of hyaluronic acid, with an initial kinematic viscosity of 50000mm 2 / s, add 20% sodium hydroxide solution under stirring to adjust the pH to 11, and degrade to a kinematic viscosity of 35000mm under stirring 2 / s, neutralized with hydrochloric acid to pH 6-7 to obtain a degradation solution, and the molecular weight of hyaluronic acid was measured to be 1800kDa.

[0092] (2) Add 1L of ethanol to the degradation solution, so that the ethanol volume concentration is 47% to produce a precipitate, stir for 0.5h, let stand for 0.5h, pour off the supernatant, and dehydrate the precipitate with ethanol to a volume concentration of 95%, after dehydration The precipitate was dried in an oven at 45°C until the water content was about 3%, and then put into a pulverizer and pulverized into powder to obtain a hyaluronic acid powder containing broken bacteria. In terms of the mass ...

technical field

[0001] The invention relates to the technical field of pet food, in particular to a pet molar stick composition containing hyaluronic acid, a preparation method and an application thereof. Background technique

[0002] With the continuous improvement of the living standards of Chinese residents and the reduction of family size, keeping pets has become a part of the life of more and more urbanites. People's growing love for pets has led to a rapid increase in the number of pets, which in turn has prompted the gradual growth of the pet food industry. In pet food, the demand for molar sticks is increasing day by day. Teething sticks can avoid or reduce pet damage to furniture. More importantly, molar sticks can help remove food residues on pet teeth, control the accumulation of tartar, and remove pets. Dental plaque, keeps gums healthy, helps eliminate bad breath in pet dogs, maintains white teeth, and reduces potential dental serious risks.

[0003] The patent...

Examples

preparation example Construction

[0053] The invention provides a preparation method of a pet molar stick composition, which comprises the following steps:

[0054] Adding hyaluronidase, acid or alkali to the hyaluronic acid fermentation broth containing bacteria used to ferment and produce hyaluronic acid to degrade to obtain a degradation solution;

[0055] Precipitate the degradation solution to obtain a precipitate, and dry the precipitate to obtain hyaluronic acid or its salt containing broken bacteria;

[0056] The pet molar stick composition is obtained by mixing the hyaluronic acid or its salt containing broken bacteria and meat powder.

[0057] In a preferred embodiment of the present invention, wherein the hyaluronidase is selected from hyaluronidase derived from bovine testis, hyaluronidase derived from Mianyang testis, hyaluronidase derived from microorganisms, recombinant One or more of human hyaluronidase and chondroitinase ABC;

[0058] The microorganism-derived hyaluronidase may be, for examp...

Embodiment 1

[0086] (1) Take 10L of hyaluronic acid fermentation broth containing bacteria for fermentation and production of hyaluronic acid, with an initial kinematic viscosity of 50000mm 2 / s, add 300 μL of hyaluronidase (enzyme activity 6000IU / mL) under stirring, hydrolyze in a water bath at 37°C for 2h, heat to 60°C to inactivate for 0.5h, and the final kinematic viscosity of the degradation solution is 138mm 2 / s, the measured molecular weight of hyaluronic acid is 400kDa.

[0087] (2) Add 30L ethanol to the degradation solution until the volume concentration is 70% to produce a precipitate, stir for 0.5h, let stand for 0.5h, pour off the supernatant, dehydrate the precipitate with ethanol to a volume concentration of 90%, and place the precipitate after dehydration Oven-dried at 50°C until the water content is about 18%, put it into a pulverizer and pulverize it into powder to obtain hyaluronic acid powder containing broken bacteria, wherein the hyaluronic acid or its salt containin...

Embodiment 2

[0091] (1) Take 1L of hyaluronic acid fermentation broth containing bacteria for fermentation and production of hyaluronic acid, with an initial kinematic viscosity of 50000mm 2 / s, add 20% sodium hydroxide solution under stirring to adjust the pH to 11, and degrade to a kinematic viscosity of 35000mm under stirring 2 / s, neutralized with hydrochloric acid to pH 6-7 to obtain a degradation solution, and the molecular weight of hyaluronic acid was measured to be 1800kDa.

[0092] (2) Add 1L of ethanol to the degradation solution, so that the ethanol volume concentration is 47% to produce a precipitate, stir for 0.5h, let stand for 0.5h, pour off the supernatant, and dehydrate the precipitate with ethanol to a volume concentration of 95%, after dehydration The precipitate was dried in an oven at 45°C until the water content was about 3%, and then put into a pulverizer and pulverized into powder to obtain a hyaluronic acid powder containing broken bacteria. In terms of the mass ...

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