Method for extracting plant selenoprotein from selenium-rich rice

A technology for plant selenoprotein and selenium-enriched rice, which is applied in the fields of peptide preparation, chemical instruments and methods, organic chemistry, etc., can solve problems such as the loss of selenoprotein, and achieve the effects of increasing content, improving extraction efficiency, and improving extraction rate.

Active Publication Date: 2017-11-17
AGRI RESOURCE & ENVIRONMENT RES INST GUANGXI ACADEMY OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The solvent extraction method has been widely promoted due to its high efficiency and low cost, but at the same time there is also the problem of

Method used

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  • Method for extracting plant selenoprotein from selenium-rich rice
  • Method for extracting plant selenoprotein from selenium-rich rice

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Embodiment 1

[0029] 1. Preliminary preparation:

[0030] Preparation of amylase preparation: in parts by weight, mix 100 parts of fresh corn and 100 parts of deionized water, put them in a reactor, and after homogenization, introduce carbon monoxide at a rate of 0.01mL / min. Air for 10 minutes, centrifuge at a speed of 5000r / min for 5 minutes, take the supernatant and adjust the pH value to 5, add 100 parts of ethanol for precipitation, after standing for 6 hours, dry the precipitate to obtain an amylase preparation.

[0031] The substances prepared above are used in the following method for extracting phytoselenoproteins from selenium-enriched rice.

[0032] 2. a method for extracting plant selenoprotein from selenium-enriched rice, comprising the following steps:

[0033] (1) Pretreatment: in parts by weight, 200 parts of selenium-enriched rice was soaked in 1000 parts of 2% acetic acid solution by mass fraction for 4 hours, ground into a slurry, added 1 part of amylase preparation, and ...

Embodiment 2

[0037] 1. Preliminary preparation:

[0038] Preparation of amylase preparation: in parts by weight, after mixing 100 parts of fresh corn and 105 parts of deionized water, put them into a reactor, and after homogenization treatment, carbon monoxide was introduced at a rate of 0.012 mL / min. Air for 13 minutes, centrifuge at 6000r / min for 6 minutes, take the supernatant and adjust the pH value to 5.5, add 140 parts of acetone for precipitation, after standing for 6.5 hours, dry the precipitate to obtain amylase preparation.

[0039] The substances prepared above are used in the following method for extracting phytoselenoproteins from selenium-enriched rice.

[0040] 2. a method for extracting plant selenoprotein from selenium-enriched rice, comprising the following steps:

[0041] (1) Pretreatment: In parts by weight, 200 parts of selenium-enriched rice is soaked in 1000 parts of acetic acid solution with a mass fraction of 2.5% for 5 hours, ground into a slurry, and after addin...

Embodiment 3

[0045] 1. Preliminary preparation:

[0046] Preparation of amylase preparation: in parts by weight, mix 100 parts of fresh corn and 110 parts of deionized water, put them into a reactor, and after homogenization, carbon monoxide is introduced at a rate of 0.015 mL / min. Air for 14 minutes, centrifuge at 6000r / min for 7 minutes, take the supernatant and adjust the pH value to 6, add 190 parts of methanol for precipitation, after standing for 7 hours, dry the precipitate to obtain amylase preparation.

[0047] The substances prepared above are used in the following method for extracting phytoselenoproteins from selenium-enriched rice.

[0048] 2. a method for extracting plant selenoprotein from selenium-enriched rice, comprising the following steps:

[0049] (1) Pretreatment: In parts by weight, 200 parts of selenium-enriched rice is placed in 1000 parts of selenium-enriched rice and soaked in 4% acetic acid solution for 4.5 hours, ground into a slurry, and after adding 2 parts ...

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Abstract

The invention discloses a method for extracting plant selenoprotein from selenium-rich rice. The method comprises steps as follows: (1), pretreatment; (2), extraction treatment: slurry obtained after enzymolysis is mixed with an extract liquid, a mixture is subjected to far-infrared ray irradiation treatment as assistance, subjected to water bath oscillation for 1-6 h and centrifuged for 15-22 min at the temperature of 25 DEG C at the rotating speed of 3000-5000 r/min, and an obtained supernatant is a protein liquid; (3), deposition treatment: the protein liquid is enabled to be under the condition of ice bath, icy acetone 4 times the volume of the protein liquid is added, then a mixed liquid is put in an environment at the temperature of subzero 27 DEG C-subzero 17 DEG C and is placed for 3-5 h under the assistant treatment condition that sound energy density of ultrasonic waves is 10-18 W/mL for selenoprotein deposition, the mixed liquid is centrifuged for 10-15 min, precipitates are taken out and subjected to spray drying, and finally, finished selenoprotein products are obtained. The extracted plant selenoprotein has the advantages of small grain diameter and high extraction rate and the method has broad market popularization value.

Description

【Technical field】 [0001] The invention relates to the field of extracting plant selenoproteins, in particular to a method for extracting plant selenoproteins from selenium-enriched rice. 【Background technique】 [0002] Selenium is one of the essential trace elements for humans and animals. Selenium can improve immunity and accelerate the production of immunoglobulins and antibodies. Protect cardiovascular and myocardial, reduce the incidence of cardiovascular disease and angina pectoris. It has various functions in anti-oxidation, anti-aging, anti-cancer and anti-cancer, and has special physiological functions such as reducing heavy metal toxicity. It is praised by domestic and foreign scientists as "the king of anti-cancer, the element of longevity, the fire of life, the natural antidote, and the patron saint of the heart". [0003] Selenium can be divided into organic selenium and inorganic selenium. Inorganic selenium is mainly selenate and selenite, accounting for 8% o...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/30
CPCC07K1/145C07K1/30
Inventor 刘永贤兰秀潘丽萍农梦玲梁潘霞黄雁飞鹿士杨陈锦平尹艳镇王道波
Owner AGRI RESOURCE & ENVIRONMENT RES INST GUANGXI ACADEMY OF AGRI SCI
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