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A kind of precipitation process in the process of extracting selenoprotein

A technology of selenoprotein and process, which is applied in the field of precipitation process, can solve the problems of low precipitation efficiency of alcohol precipitants, and achieve the effects of facilitating human body absorption, avoiding agglomeration, and high-efficiency precipitation

Active Publication Date: 2020-06-19
AGRI RESOURCE & ENVIRONMENT RES INST GUANGXI ACADEMY OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research direction of selenoproteins is mainly concentrated on the extraction, and there are few studies on the precipitation of selenoproteins. The precipitants commonly used in the market are mainly alcohols and ketone precipitants, and ketone precipitants are widely used. , but the alcohol precipitant has the problem of low precipitation efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preliminary preparation:

[0023] Preparation of selenoprotein extract: in parts by weight, soak 200 parts of selenium-enriched rice in 800 parts of deionized water at 60°C for 3 hours, grind it into a slurry, add 1 part of amylase, and enzymatically hydrolyze it at 25°C for 10 hours , and then add 1000 parts of the extract, extract at a temperature of 45°C for 10 hours, then put it into a centrifuge and centrifuge at a speed of 3000r / min for 10 minutes, and the obtained supernatant is the selenoprotein extract.

[0024] Preparation of protective gas: Mix nitrogen and inert gas at a volume ratio of 10:3 to obtain a protective gas.

[0025] The substances prepared above are used in the precipitation process in the following process of extracting selenoproteins.

[0026] 2. A precipitation process in the process of extracting selenoproteins, prepared by the following method:

[0027] Put the selenoprotein extract into a low-temperature reactor, lower the temperature ...

Embodiment 2

[0030] 1. Preliminary preparation:

[0031] Preparation of selenoprotein extract: in parts by weight, soak 200 parts of selenium-enriched rice in 800 parts of deionized water at 65°C for 3.5 hours, grind it into a slurry, add 2 parts of amylase, and enzymolyze it at 28°C 12h, then add 1500 parts of extracting solution, extract at a temperature of 50°C for 15h, then put it into a centrifuge, and centrifuge at a speed of 3500r / min for 13min, the obtained supernatant is the selenoprotein extract.

[0032] Preparation of protective gas: Mix nitrogen and inert gas at a volume ratio of 5:2 to obtain a protective gas.

[0033] The substances prepared above are used in the precipitation process in the following process of extracting selenoproteins.

[0034] 2. A precipitation process in the process of extracting selenoproteins, prepared by the following method:

[0035]Put the selenoprotein extract into a low-temperature reactor, lower the temperature below 0°C at a cooling rate of ...

Embodiment 3

[0038] 1. Preliminary preparation:

[0039] Preparation of selenoprotein extract: in parts by weight, soak 200 parts of selenium-enriched rice in 800 parts of deionized water at 68°C for 4 hours, grind it into a slurry, add 1.5 parts of amylase, and enzymatically hydrolyze it at 30°C for 14 hours , and then add 2000 parts of the extract, extract at a temperature of 60°C for 15 hours, then put it into a centrifuge and centrifuge at a speed of 4000r / min for 14 minutes, and the supernatant obtained is the selenoprotein extract.

[0040] Preparation of protective gas: Mix nitrogen and inert gas at a volume ratio of 5:3 to obtain a protective gas.

[0041] The substances prepared above are used in the precipitation process in the following process of extracting selenoproteins.

[0042] 2. A precipitation process in the process of extracting selenoproteins, prepared by the following method:

[0043] Put the selenoprotein extract into a low-temperature reactor, lower the temperatur...

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PUM

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Abstract

The invention discloses a novel precipitation technology in selenoprotein extraction process. The novel precipitation technology comprises the following steps: putting a selenoprotein extract liquor into a low-temperature reactor, decreasing the temperature to be below 0 DEG C at the cooling rate of 3 to 5 DEG C so that the selenoprotein extract liquor is put in an ice-bath environment, adding the selenoprotein extract liquor into iced acetone with the volume being four times of that of the selenoprotein extract liquor at the rate of 1 to 4 mL / min, simultaneously, introducing protective gas into the selenoprotein extract liquor and starting a three-level stirring procedure, firstly stirring for 2 to 3 min at the stirring rate of 1000 to 2000 r / min, then stirring for 4 to 6 min at the stirring rate of 500 to 800 r / min, then stirring for 4 to 6 min at the stirring rate of 100 to 200 r / min, stopping ventilation after stirring is ended, cooling to be -27 to -17 DEG C, putting for 3 to 5 h under the assistant treatment condition that the sound energy density of ultrasonic wave is 15 to 25 W / mL, and after drying and smashing a precipitate, obtaining selenoprotein. The selenoprotein can be efficiently precipitated under the condition that the quality of selenoprotein is guaranteed, and the novel precipitation technology has great market popularization value.

Description

【Technical field】 [0001] The invention relates to the field of precipitation of selenoproteins, in particular to a precipitation process in the process of extracting selenoproteins. 【Background technique】 [0002] Selenium is one of the essential trace elements for humans and animals. Selenium can improve immunity and accelerate the production of immunoglobulins and antibodies. Protect cardiovascular and myocardial, reduce the incidence of cardiovascular disease and angina pectoris. It has various functions in anti-oxidation, anti-aging, anti-cancer and anti-cancer, and has special physiological functions such as reducing heavy metal toxicity. It is praised by domestic and foreign scientists as "the king of anti-cancer, the element of longevity, the fire of life, the natural antidote, and the patron saint of the heart". [0003] Selenium can be divided into organic selenium and inorganic selenium. Inorganic selenium is mainly selenate and selenite, accounting for 8% of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C07K1/30
CPCC07K1/30C07K14/415
Inventor 刘永贤农梦玲梁潘霞兰秀潘丽萍黄雁飞鹿士杨陈锦平尹艳镇王道波
Owner AGRI RESOURCE & ENVIRONMENT RES INST GUANGXI ACADEMY OF AGRI SCI
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