Extraction device and technology of selenium polypeptide from soybeans

An extraction device and process technology, which is applied in the field of extraction devices for extracting selenium polypeptides, can solve the problems of low efficiency and poor quality of selenium polypeptides, and achieve the effect of solving numerous extraction procedures, less impurities and high purity of selenium polypeptides

Pending Publication Date: 2020-01-31
ENSHI JUXIN MODERN AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the technical problems of low efficiency and poor quality in the extraction of selenium polypeptides fro...

Method used

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  • Extraction device and technology of selenium polypeptide from soybeans
  • Extraction device and technology of selenium polypeptide from soybeans

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Experimental program
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Embodiment 1

[0028] Embodiment 1: According to figure 1 , an extraction device and process for extracting selenopolypeptides from soybeans, comprising: step 1) preparing materials, step 2) extracting selenoprotein powder, step 3) mixing slurry, step 4) ultrasonic enzymolysis, step 5) modifying, Step 6) removing impurities, and step 7) concentrating and drying.

[0029] Described process comprises the following steps:

[0030] 1) Prepare materials, select mature soybeans rich in selenium, pass through a vibrating sieve to remove sand and stones, dry to remove the outer skin, and remove oil. The oil content of soybean meal after oil removal is less than 3%, and crushed into soybean powder with a size of 80 mesh;

[0031] 2) To extract protein, mix soybean powder with water at a solid-to-liquid weight ratio of 1:20-25, adjust the Ph value to 9.5 by adding sodium hydroxide solution, and leaching for 5 hours at a temperature of 45-60°C. Slowly stir in the medium, and after the leaching is com...

Embodiment 2

[0044] Embodiment 2, described membrane filtration adopts twice to filter, adopts membrane hole to be 50-300 μm microporous membrane for the first time, filter out macromolecular protein, adopts membrane hole to be less than 15 μm microporous membrane for the second time, intercepts small Molecular peptides, filtering out smaller amino acids.

[0045] In addition to filtering out large molecular proteins, smaller amino acid molecules are also filtered out to increase the purity of peptides.

Embodiment 3

[0046] Embodiment 3, described technique comprises the following steps:

[0047] 1) Prepare materials, select mature soybeans rich in selenium, pass through a vibrating sieve to remove sand and stones, dry to remove the outer skin, and remove oil. The oil content of soybean meal after oil removal is less than 3%, and crushed into soybean powder with a size of 80 mesh;

[0048] 2) To extract protein, mix soybean powder with water at a solid-to-liquid weight ratio of 1:20, adjust the Ph value to 9.5 by adding sodium hydroxide solution, and leaching for 5 hours at a leaching temperature of 60°C, stirring during leaching , stirred at 40 rpm, and after the leaching was completed, solid-liquid filtration and separation were carried out;

[0049] Secondary leaching: carry out secondary leaching on the leached slag, mix soybean powder with water at a solid-to-liquid weight ratio of 1:15, adjust the Ph value to 9.5 by adding sodium hydroxide solution, and leaching for 3 hours , the le...

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Abstract

The invention discloses an extraction device and technology of selenium polypeptide from soybeans. The extraction technology comprises the steps of step (1) preparing materials; step (2) extracting selenium protein powder; step (3) performing pulp mixing; step (4) performing ultrasonic enzymolysis; step (5) performing modification; step (6) removing impurities; and step (7) performing concentration and drying. According to the extraction device and technology, the selenium protein is extracted firstly, then the emulsion is subjected to enzymolysis twice and modified, the extracted selenium polypeptide is low in the number of impurities, the purity is high, the selenium polypeptide is free from peculiar smell, and the technical problem that in the prior art, many extraction processes are used, inconvenient to use and low in efficacy can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation extraction, and in particular relates to an extraction device and process for extracting selenium polypeptides from soybeans. Background technique [0002] Soybeans are rich in protein. Soybeans grown in selenium-rich areas such as western Hubei and southern Shaanxi contain a large amount of selenoproteins. Since large molecular proteins are not easily absorbed by the human body, it is easy to transform large molecular selenium-rich proteins into It is a small molecule selenium polypeptide absorbed directly by the human body. [0003] Selenium polypeptides can be used as additives in skin care products, food, medicine and health products, etc., and have the effects of anti-oxidation, anti-aging, prevention of diabetes, prevention of cardiovascular and cerebrovascular diseases and beauty. [0004] At present, for the processing of soybean selenoprotein isolation, methods such as ac...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14C07K1/34C12M1/42C12M1/40C12M1/12C12M1/02C12M1/00
CPCC12P21/06C12M21/18C12M27/02C12M29/04C12M35/04C12M41/24C12M45/05C12M45/06
Inventor 南占东王成赵静黄毅华
Owner ENSHI JUXIN MODERN AGRI DEV
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