Method for extracting selenoprotein from selenium-rich edible fungi

Abstract

The invention discloses a method for extracting selenoprotein from selenium-rich edible fungi, relates to the field of selenoprotein extraction, and aims to solve the problem that natural edible fungiare quite low in selenium content and needs to be supplemented with the selenium content. The method specifically comprises the following steps: step one, preparing selenium-rich edible fungi; step two, acid extraction; step three, enzymolysis; step four, preparation of crude selenoprotein liquid; step five, preparation of a finished product: adding the crude selenoprotein liquid into an ethanolsolution of which the mass fraction is 88-94% and the volume is 3-5 times that of the crude selenoprotein liquid for alcoholysis to obtain precipitates, and sequentially washing the precipitates withabsolute ethyl alcohol, petroleum ether and acetone and carrying out vacuum drying to obtain the finished product. According to the invention, selenium is enriched in the edible fungi and converted into organic selenium, selenoprotein is obtained by means of enzymolysis, centrifugation, filtration and the like, the extraction rate of the selenoprotein is high, and the purity of the obtained selenoprotein is high. The finished product obtained in the invention can be applied to the field of food processing, meets demands of selenium supplementation of human bodies, and has wide application prospect.

Description

technical field

[0001] The invention relates to the field of selenoprotein extraction, in particular to a method for extracting selenoprotein from selenium-enriched edible fungi. Background technique

[0002] Selenium is one of the essential trace elements for the human body. Selenium can improve immunity and accelerate the production of immunoglobulins and antibodies. Protect cardiovascular and myocardial, reduce the incidence of cardiovascular disease and angina pectoris. It has multiple functions in anti-oxidation, anti-aging, anti-cancer and anti-cancer, and has special physiological functions such as reducing the toxicity of heavy metals. Elixir, Patronus of the Heart". Many diseases are closely related to the nutritional status of selenium.

[0003] Due to the uneven distribution of selenium on the earth, it is currently known that more than 40 countries and regions are deficient in selenium to varying degrees, and more than 2 / 3 of my country is deficient in seleniu...