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Method for extracting selenoprotein from selenium-rich rice bran

A technology of selenium-enriched rice and selenoprotein, applied in the field of selenoprotein extraction, can solve the problems of less than 20% effective utilization rate of rice bran, high requirements for reaction conditions, and high process cost, and achieve wide extraction temperature range, good absorption effect, and high extraction efficiency. high efficiency effect

Inactive Publication Date: 2017-05-31
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effective utilization rate of rice bran is still less than 20%, and most of them are used as feed, or even as waste, which is a serious waste of resources.
The 2014 patent "A method of enzymatically treating selenium-enriched rice to produce selenium-enriched protein powder (201410690328.0)" discloses the use of enzymes to prepare protein powder. Although the reaction conditions are relatively mild, the enzymes are easily inactivated, and the requirements for reaction conditions are relatively high. High, the extraction time is long, and the process cost is also high

Method used

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  • Method for extracting selenoprotein from selenium-rich rice bran
  • Method for extracting selenoprotein from selenium-rich rice bran
  • Method for extracting selenoprotein from selenium-rich rice bran

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Raw material and processing: Wash the fresh selenium-enriched rice bran with distilled water, put it into an oven for drying; grind it with a rice huller, and pass it through a 100-mesh sieve.

[0031] (2) Dosing: prepare a sodium hydroxide solution with a concentration of 0.01mol / L, mix hydrosulfite and dithiothreitol in a mass ratio of 1:2 and add them to the sodium hydroxide solution, and the amount of hydrosulfite is as follows: (1) 0.5% of the mass of the prepared rice bran powder.

[0032] (3) Ultrasonic extraction: mix the rice bran powder prepared in step (1) with the extraction solution prepared in step (2) according to the ratio of mass to volume of 1:5, and put it into an ultrasonic cleaning machine for ultrasonic extraction, which greatly improves the extraction efficiency , set the extraction temperature to 35° C., the ultrasonic frequency to 100 Hz, and the extraction time to 3 hours to obtain an ultrasonic extraction solution.

[0033] (4) Decoloriza...

Embodiment 2

[0044] (1) Raw material and processing: Wash the fresh selenium-enriched rice bran with distilled water, put it into an oven for drying; grind it with a rice huller, and pass it through a 100-mesh sieve.

[0045] (2) Dosing: prepare a sodium hydroxide solution with a concentration of 0.04mol / L, mix sodium hydrosulfite and dithiothreitol in a mass ratio of 1:4 and add it to the sodium hydroxide solution, and increase dithiothreitol amount to extract more protein. The consumption of hydrosulfite is 0.5% of the quality of the rice bran powder that step (1) makes.

[0046] (3) Ultrasonic extraction: mix the rice bran powder prepared in step (1) with the extraction solution prepared in step (2) at a ratio of 1:10 in mass to volume, and increase the ratio of solid to liquid to make the extraction more complete. Then put it into an ultrasonic cleaning machine for ultrasonic extraction, the extraction temperature is 45° C., the ultrasonic frequency is 100 Hz, and the extraction time ...

Embodiment 3

[0055] (1) Raw material and processing: Wash the fresh selenium-enriched rice bran with distilled water, put it into an oven for drying; grind it with a rice huller, and pass it through a 100-mesh sieve.

[0056] (2) Dosing: prepare a sodium hydroxide solution with a concentration of 0.05mol / L, mix hydrosulfite and dithiothreitol into the sodium hydroxide solution in a mass ratio of 1:5, and the amount of hydrosulfite is as follows: (1) 0.5% of the mass of the prepared rice bran powder.

[0057] (3) Ultrasonic extraction: mix the rice bran powder prepared in step (1) with the extract prepared in step (2) at a ratio of 1:15 in mass to volume, further increase the ratio of solid to liquid, and extract selenoproteins more fully. Put the mixed solution into an ultrasonic cleaning machine for ultrasonic extraction, further adjust the optimal extraction temperature to 40°C, increase the extraction rate, ultrasonic frequency 100Hz, and extraction time 3 hours to obtain an ultrasonic ...

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Abstract

The invention discloses a method for extracting selenoprotein from selenium-rich rice bran. The method comprises the following steps of performing ultrasonic extraction on fresh selenium-rich rice bran powder and a prepared extraction solution in an ultrasonic washing machine; then performing decoloring and performing centrifugation for precipitation; adjusting isoelectric points with citric acid, and performing centrifugation for collecting precipitate; removing impurities with a dialysis bag; and finally, performing freeze drying so as to obtain the selenoprotein. According to the method disclosed by the invention, a manner of reducing extraction and performing acid precipitation improved, so that selenoprotein is obtained, and the protein extraction rate is 80% or above; and the extracted selenoprotein is few in impurities, the protein content is as high as 80-95%, and the selenium content of the selenoprotein is as high as 0.50-0.58mg / kg. The method is simple and convenient, conditions are mild, the production technology is simple, industrial popularization is easy and the method has high utilization value.

Description

technical field [0001] The invention relates to a method for extracting selenoprotein from rice bran of selenium-enriched rice, which belongs to the field of deep processing of functional food. Background technique [0002] China is the largest rice producer in the world, with an annual output of about 185 million tons. As a by-product of rice processing, rice bran (including cortex and germ) has an annual output of more than 10 million tons, and the available resources are quite abundant. Although rice bran only accounts for 6% to 8% of the mass of rice, it contains the main nutrients in rice and is known as the "treasure house of natural nutrition". At present, the effective utilization rate of rice bran is still less than 20%, and most of them are used as feed, or even as waste, which is a serious waste of resources. Rice bran protein mainly includes albumin, globulin, glutenin and prolamin, and rice bran protein has complete essential amino acids and high biological pot...

Claims

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Application Information

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IPC IPC(8): A23J1/00
Inventor 梁元可朱建清曾睿唐志臣张玉洁叶小英贾晓梅默罕默德张旭
Owner SICHUAN AGRI UNIV
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