99results about "Protein composition from unicellular algae" patented technology

Protein concentrates and protein isolates, in addition to processes for the production of protein concentrates and protein isolates, are disclosed. In particular, the disclosure relates to the removal of fiber from macroalgae and / or microalgae using low g-force centrifugation.

A process for preparing non-odour white silkworm pupa protein from the degreased silkworm pupa powder includes dissolving saponizing, separation, regulating isopotential point, separating, washing, deodouring and cross-linking. Its advantages are high extracting rate, and high quality of product.

The invention provides a method for extracting a tea protein product. The method specifically comprises the following steps of: 1) pretreating raw materials; 2) preparing tea protein through ultrasonic-assisted alkali extraction; 3) decolorizing a tea protein solution; 4) concentrating a tea protein extracting solution; 5) precipitating the tea protein; and 6) washing the tea protein, refining and drying. The method has the advantages that: tea residue or low-grade tea such as summer-autumn tea and the like is used as a raw material, and a low-frequency ultrasonic circulation technology is used for assisting in extracting the tea protein; by the method, the protein is high in extraction rate and has high purity; the method is low in cost, simple and practicable, and easy to popularize; and the obtained tea protein product has green tea color, and the quality of the product meets food hygienic standard. A novel way of value-added transformation and recycling of tea residue and summer-autumn low-grade tea is provided; and a novel botanical protein product is developed, and the comprehensive utilization and fine and deep processing of tea resources are realized.

The invention provides a technology for extracting high-purity potato protein through a hot flocculence method. At the significant end of the technology, small-granule starch and a fine-fiber mixture in potato starch processing and separation juice are separated, the purity and quality of the potato protein obtained through flocculence and separation at the final phase are improved, and meanwhile the phenomenon that heat exchange equipment and a pipeline are blocked due to starch dextrinization in the follow-up process is avoided; the potato protein is degenerated and flocculated through the hot flocculence method to be changed from the starch separation juice hydrosol body state into flocculence body solid to facilitate solid and liquid separation, the quality of the extracted protein is ensured, and meanwhile the physical flocculence method and the chemical flocculence precipitation method are used in a combined mode in cooperation with flocculence of chemical auxiliaries, so that the protein extraction rate and the protein separation effect are greatly improved, and the potato protein purity is improved. In addition, resource utilization of the potato protein is performed, more than 50% pollutant loads are reduced for follow-up sewage treatment, and the dual effects of resource utilization and pollutant control are achieved.

The invention provides a process for extracting yam mucoproteins from yams and belongs to the field of extracting functional biological macromolecular substances from natural materials to produce functional healthcare food. The extracting process comprises the main steps of (1) material selection; (2) leaching gelatinization; (3) enzymolysis; (4) standing; (5) adjustment of PH value; (6) flocculation; (7) precipitation; and (8) drying. The yield of the yam mucoprotein products prepared by the process is 4 to 5 percent, and the content of yam mucoprotein is 60 to 67 percent. According to the process provided by the invention, the organic solvent extraction in the prior art is replaced by the flocculation and natural precipitation, so the product cost is greatly saved, the environmental pollution is lowered, and the process has great significance for actual production.

The invention relates to light-yellow odourless degreasing silkworm pupa protein power and a preparation method thereof. According to the technical scheme, silk reeling silkworm pupae of which the skin and the gland are removed are selected as the raw material, silkworm pupa pretreatment is conducted, and coarse silkworm pupa powder is obtained; the coarse silkworm pupa powder is evenly mixed in degreasing solvent, ultrasonic degreasing treatment is conducted, centrifugal separation is conducted, and degreasing fluid and degreasing coarse pupa protein are obtained; drying and smashing are conducted on the degreasing coarse pupa protein, and the degreasing coarse pupa protein power is obtained; the degreasing coarse pupa protein power is evenly mixed in a sodium hydroxide solution, a silkworm pupa protein extracting solution is obtained, ultrasonic extraction treatment is conducted, macroporous resin is added into the silkworm pupa protein extracting solution, adsorption treatment is conducted, solid-liquid separation is conducted, resin and protein fluid are obtained, the protein fluid pH is adjusted, protein precipitation is collected, and wet silkworm pupa protein is obtained; vacuum drying and smashing are conducted on the wet silkworm pupa protein, and the light-yellow odourless degreasing silkworm pupa protein power is obtained. The light-yellow odourless degreasing silkworm pupa protein power and the preparation method thereof have the advantages that the technology is simple, the degreasing efficiency is high, effects of deodorization and decolorization are good, the treatment is low in temperature and short in time, the cost is low, the green and environmental protection is achieved, and the light-yellow odourless degreasing silkworm pupa protein power and the preparation method thereof can be used for industrial production.

The invention provides a method for extracting yellow mealworm active proteins by using an alkali-enzyme method, comprising the following steps: drying fresh yellow mealworm larvae to obtain dried yellow mealworm larvae of which the moisture content is lower than 5%, and smashing the dried yellow mealworm larvae; carrying out secondary digestion by taking petroleum ether of which the relative density is 0.64-0.66 as an extraction solvent; carrying out digestion with a 0.08-0.10mol / L NaOH solution; regulating the pH value to 7.0-7.5, carrying out enzymatically hydrolyzed extraction at a temperature of 55-60 DEG C by complex enzyme which is composed of neutral protease and papain at the mass ratio of 1:1-1:1.5; destroying enzyme; and centrifuging and dialyzing to obtain yellow mealworm protein powder. The yellow mealworm protein powder has the advantages of light yellow color and luster, beauty, fragrant odor as well as fine and smooth taste; and the protein content of the yellow mealworm protein powder reaches 94.5% and the extraction efficiency of the yellow mealworm protein powder reaches 75%.

The invention relates to a method for extracting macadimia nut proteins. The method mainly comprises the following steps of (1) smashing macadimia nuts, and separating oil of the macadimia nuts through infrared treatment and hydraulic cold squeezing; (2) mixing the obtained macadimia nut meal with pure water, treating the mixture through a colloid mill and an alkaline hardening, quenching and homogenizing machine, and then performing dynamic ultrahigh pressure treatment; and (3) adjusting the pH of the fruit meal to an isoelectric point to realize separation, and performing low-temperature spray drying to obtain the macadimia nut proteins. The technology disclosed by the invention is continuous and compact; the product is high in yield and high in quality.

The invention discloses a method for preparing semen cannabis protein powder from degreased semen cannabis residue, relating to the technical field of development and application of semen cannabis. The method comprises the steps of with degreased semen cannabis residue as a raw material, carrying out hot water protein extraction, filtrating, concentrating filtrate, and drying under the assistance of a drying aid to obtain the semen cannabis protein powder. According to the method, hot water is used as an extractant to improve the extraction efficiency of semen cannabis protein; as impurities such as an organic solvent and a acid-base reagent are not added, the extracted semen cannabis protein is not required to be purified, and the remaining semen cannabis residue is not polluted and can be used for preparing byproducts such as feeds or fertilizers, and therefore, the comprehensive utilization value of the semen cannabis is improved.

A process for preparing the vegetative protein from the seed dregs of Jatropha curcas tree, which are generated by squeezing oil, includes such steps as diluting the seed dregs by water, adding modifier (the mixed solution of H2O2 and NH4OH), heating to 100-120 deg.C and detoxicating reaction. Said vegetative protein can be used as the additive of feed.

The invention discloses a method for extracting selenoprotein from selenium-rich rice bran. The method comprises the following steps of performing ultrasonic extraction on fresh selenium-rich rice bran powder and a prepared extraction solution in an ultrasonic washing machine; then performing decoloring and performing centrifugation for precipitation; adjusting isoelectric points with citric acid, and performing centrifugation for collecting precipitate; removing impurities with a dialysis bag; and finally, performing freeze drying so as to obtain the selenoprotein. According to the method disclosed by the invention, a manner of reducing extraction and performing acid precipitation improved, so that selenoprotein is obtained, and the protein extraction rate is 80% or above; and the extracted selenoprotein is few in impurities, the protein content is as high as 80-95%, and the selenium content of the selenoprotein is as high as 0.50-0.58mg / kg. The method is simple and convenient, conditions are mild, the production technology is simple, industrial popularization is easy and the method has high utilization value.

The invention discloses a production method of a non-denatured pure sericin stock solution. By utilizing the production method, the pure sericin stock solution is produced by evading sericin denatured conditions. The production method is simple, low in cost and suitable for production of nutritious foods, health care products and products in other fields.

The invention provides a method for producing cottonseed germ materials used in low-temperature pretreatment production of gossypol-removed cottonseed protein. The method comprises: cleaning and removing impurities in delinted cottonseeds; husking the cottonseeds after impurity removal; obtaining mixture of cottonseed kernels and cottonseed husks; repeatedly separating the mixture by use of a screening and / or winnowing method; obtaining the cottonseed kernels in which the content of the cottonseed husks is less than or equal to 18 percent; softening the cottonseed kernels at a temperature lower than or equal to 80 DEG C; rolling the cottonseed kernels into germ sheets; adjusting quality at a temperature lower than or equal to 80 DEG C; and obtaining germ materials. The germ materials produced by use of the method have the advantages of good shape, high strength, less breakable property, good permeability during extraction, short seepage time, low content of residual oil and gossypol in meal after oil extraction and gossypol removal, capability of continuous production and capability of maintaining the original nutritional value of protein.

The invention discloses a method for extracting active protein from rough old tea. The method comprises the steps of destroying the cell wall of tea cell through ultrasonic-assisted extraction, cellulose treatment and pectase treatment, so as to ensure that active material is dissolved out to a maximum limit, extricating acidic protein in the tea through alkaline process, separating out tea protein through an isoelectric precipitation method, and performing zymohydrolysis to the tea protein, so as to improve performances of the tea protein again. As ultrasonic technology and complex enzyme treatment destroy the cell wall of the tea cell, and the structure of active material in the cell is also affected to a certain degree, the yield of tea protein samples obtained through the method is high; and after zymohydrolysis treatment, the solubility and the antioxidant activity of protein are obviously improved, so that the method has the advantages of high resource utilization rate, energy conservation, enhanced activity and the like.

The invention provides a preparation method of chlorella concentrate phycocyanin functional beverage and belongs to the field of biotechnology. The preparation method comprises the following steps: uniformly mixing chlorella and deionized water in a ratio of 1:(20-50), heating mixed liquor for 30-60 minutes at the temperature of 110-121 DEG C, centrifuging for 15-30 minutes, then abandoning precipitates, carrying out ultrafiltration on supernatant, and carrying out freeze drying on ultrafiltrate, so that chlorella concentrate is obtained; mixing spirulina powder and water in a ratio of 1:(10-20), swelling for 24 hours, then stirring and leaching for 2-4 hours at the temperature of 25-40 DEG C and the stirring rotating speed of 100-400rpm, centrifuging a leachate for 15-30 minutes, carryingout ultrafiltration on the supernatant for removing small molecular impurities, so that phycocyanin solution with color value of higher than 0.8 is obtained, carrying out ultrafiltration on the phycocyanin solution, and carrying out free drying on ultrafiltrate, so that phycocyanin powder is obtained; mixing the obtained phycocyanin, the chlorella concentrate, white granulated sugar, food additives such as sodium benzoate and tea polyphenol as well as water, preparing into functional beverage with health function, filtering and removing bacteria with a 0.22 Mum filter membrane, and then packaging. The preparation method provided by the invention is simple and reliable in operation, and industrial production can be carried out.

The invention discloses a process for preparing fly larva albumen powder by enzymatic hydrolysis of fly larvae. The process comprises the following specific steps: adding water to fresh fly larvae, pulping, and adjusting the pH as 6.0-7.0; adding an enzyme composition, raising the temperature to 45-55 DEG C, performing heat-preservation enzymatic hydrolysis for 4-7h, and performing enzyme deactivation, filtration, filtrate collection, concentration and drying to obtain the fly larva albumen powder, wherein the enzyme composition is formed by compound protease, neutral protease and flavourzyme according to the weight ratio of 1:(1-2):(0.5-1), and the addition amount of the enzyme composition is 2-5 weight percent of the fresh fly larvae. Compared with the prior art, the process has the advantages that the enzyme composition formed by the compound protease, the neutral protease and the flavourzyme according to a specific ratio performs enzymatic hydrolysis on the pulp of the fresh fly larvae under specific conditions, all enzymes cooperate in the enzymatic hydrolysis process, the degree of hydrolysis is effectively improved, and meanwhile, the prepared fly larva albumen powder does not taste bitter, and only has slightly fishy smell.

The invention relates to a production method of a proteoglycan protein compound functional capsule product from domestic fungus / alga and belongs to the field of edible fungi and alga deep processing and related product development and application. Edible fungi polysaccharide powder and algal polysaccharide powder which are prepared by an ultrasonic-assisted extraction technology and edible fungi protein powder and alga protein powder which are prepared by a salting-in technology are compounded according to a certain ratio, and a conventional capsule preparation technology is adopted, so as to prepare the proteoglycan protein compound functional capsule product. By analyzing biological functional activities of polysaccharides and protein powders from two different sources, scientific proportioning is carried out to obtain the capsule product which has advantages of rich nutrient, comprehensive biological activity and high utilization rate. In comparison with like products, the nutritional compound capsule provided by the invention can be used to remarkably enhance immunity of the organism and delay aging process. The proteoglycan protein compound functional capsule product is a health food which has market value.

The invention discloses an extracting method for high-purity sweet potato protein. The method comprises the following steps: (1) washing and crushing a sweet potato, sieving the crushed sweet potato, adding pure water, stirring and soaking, and taking supernatant; (2) centrifuging the supernatant obtained in the step (1), and taking the supernatant; (3) filtering or performing suction filtering on the supernatant obtained by the step (2) through a food-grade activated carbon layer; (4) slowly adding the filtrate obtained in the step (3) into food-grade alcohol under the stirring condition, and standing to obtain flocculent mixed liquid; (5) centrifuging the flocculent mixed liquid which is obtained in the step (4); (6) collecting the solid obtained in the step (5), and performing vacuum drying or freeze drying to obtain finished sweet potato protein. Compared with the conventional process, the extracting method for the sweet potato protein disclosed by the invention has the advantages that the process is simple and stable, conditions are mild, the environment-friendly effect is achieved, and the purity of the obtained sweet potato protein is 92 to 97 percent, and the purity is higher compared with the purity of the sweet potato protein of 72.4 percent obtained by the conventional process method.

The invention relates to a method for preparing a protein isolate of the biomass of microalgae of the genus Chlorella, characterised in that it comprises the following steps: supplying a microalgal biomass produced by fermentation, washing the biomass so as to eliminate the soluble interstitial compounds and concentrating the biomass, mechanically grinding the washed and concentrated biomass in a horizontal ball grinder-type system in order to produce an emulsion, destructuring the emulsion thus produced, triple-phase separation so as to separate the soluble fraction from the fractions containing the lipids and the cell debris, recovery of the soluble fraction thus produced in order to produce the soluble protein isolate, then evaporation, pasteurisation and atomisation of said protein isolate.

The invention relates to a brassica oleracea protein powder preparation method, which comprises: preparing a juice by adopting at least one organ selected from the stem, the leaf and the flower of brassica oleracea as raw material, carrying out heating flocculation on the juice, filtering to obtain solid protein and a filtrate, concentrating the filtrate through a membrane, mixing the concentrated filtrate and the solid protein, homogenizing, and carrying out spray drying to obtain the brassica oleracea protein powder. According to the present invention, the method has characteristics of simple process and strong operability; and the experiment results prove that the obtained brassica oleracea protein powder has effects of lowering of cholesterol in blood fat, and can be used in the fields of food, health products, medicine, and the like.

The invention belongs to the field of health-caring foods, and especially relates to a health-caring food capable of improving osteoarthritis and a preparation method thereof. Multiple novel food raw materials and bone-nourishing traditional-Chinese-medicine raw materials are compounded so as to prepare the health-caring food used for improving osteoarthritis provided by the invention, and thus, the prepared health-caring food is capable of stimulating chondrocyte formation and bone growth, as well as eliminating cellular inflammatory response so as to relieve pain of the bone and the joints, as well as promote cartilage repair. By utilizing the synergistic effects between the multiple novel food raw materials and bone-nourishing traditional-Chinese-medicine raw materials, the prepared health-caring food has very good curative improvement effects on bone and joint injuries. The health-caring food is obvious in curative improvement effects, and free of any other side effects.

The invention relates to a peptide isolate isolated from a biomass of protein-rich microalgae, characterized in that it comprises:soluble peptides with a molecular weight of between 1 and 20 kDa,a protein content expressed as N.6.25 of more than 95%,essentially arginine and glutamic acid.

The invention discloses an extraction method of an MD (musca domestica) insect active protein intermediate, comprising the following steps: cleaning and drying MD larva; after drying the MD larva by blowing at 40 DEG C, smashing the dried product; fully degreasing; adding 1-1.5% of alkali, and adding water for hydrolysis at constant temperature; secondarily desalting; and freezing and drying leached and desalted precipitate. Because the above technical scheme is adopted, technological operation difficulty is greatly lowered and production cost is greatly lowered by changing complex dialysis desalting with high cost into the secondary desalting; in the process of secondary desalting, hydrolyzate is required to be precipitated and AgNO3 is not needed to be used for detection, which protects the health of workers; and in the working procedure of drying the original substance by blowing at 40 DEG C after cleaning and drying, considerable energy can be saved, equipment investment and manual work are reduced if compared with the alkali dilution method of the Institute of Zoology of Chinese Academy of Sciences that the original substance is boiled for 15 minutes and is frozen and dried, and thus production cost is greatly lowered.

The invention discloses a biological deodorization and discoloration method for proteins from silk-reeling silkworm chrysalises, which comprises the following steps: carrying out high-temperature sterilization on a certain amount of silkworm chrysalis powder, inoculating with a specific microorganism strain, carrying out solid fermentation, carrying out biological transformation processes of blanching, disinfection, degreasing and the like while soaking in ethanol, dehydrating, and crushing to obtain silkworm chrysalis protein powder with good color and no odor. The method has the advantage of convenient process, and is suitable for industrial processing production. The prepared silkworm chrysalis protein has no odor, and the color of the silkworm chrysalis powder is changed from puce to offwhite. The deodorized silkworm chrysalis protein has more reasonable amino acid composition, the content of seven essential amino acids is increased by 39-85%, and the content of the silkworm chrysalis protein is increased by 2-3%.

A preparation technology of porphyra haitanensis phycobiliprotein powder belongs to the processing field of food supplement, and particularly relates to the preparation technology of the porphyra haitanensis phycobiliprotein powder. The invention provides the preparation technology of the porphyra haitanensis phycobiliprotein powder, which is simple and effective, and is applicable to mass production. The preparation steps comprise: porphyra haitanensis cell disruption technology; phycobiliprotein crude extract preparation; and phycobiliprotein purification.

Provided are methods for producing food products comprising recombinant components, and compositions used in and food products produced by such methods.

The invention discloses a tea flower protein extract and an application thereof. The preparation method of the tea flower protein extract comprises the following steps: (1) drying and grinding tea flowers to obtain tea flower powder; (2) extracting the tea flower powder by using an alkaline method or an enzyme method, and taking the extract liquid; and (3) decoloring the extract liquid, adjusting the pH value to ensure that the protein precipitates and finally separating to obtain the tea flower protein extract. The tea flower extract is obtained through alkaline method extraction and enzyme method extraction, the extraction rate of the alkaline method is up to 91.45% and the extraction rate of the enzyme method is up to 79.12%, so that both the extraction rates are relatively high and the effective utilization of the tea flower resource is realized. Compared with the tea leaf protein, the tea flower protein extract has excellent water retention capacity, oil absorbency, emulsifying property, foaming characteristic and antioxidation, and can be used for preparing antioxidants, foaming agents and emulsifiers with better performance.

The present invention provides a preparation method of a selenium-rich and zinc-rich protein peptide. The preparation method comprises the following steps: screened wheat is placed in a warm double-element solution to be soaked for accelerating germination; the wheat is germinated after cultivating; the double-element solution is a mixed solution of Na2SeO3 and ZnSO4; harvested malts are placed inwarm water for enzyme deactivating; the enzyme deactivated malts are subjected to tissue homogenizing and wall breaking; cellulose is added for hydrolysis; alkaline protease is added for hydrolysis;the enzymes are inactivated; the pH is adjusted to be neutral; a malt extract is prepared; the malt extract is centrifuged to remove impurities; and then concentrating and spray drying are conducted to obtain the selenium-rich and zinc-rich protein peptide. The used wheat malts have a characteristic of gathering two elements and convert toxic inorganic selenium and zinc into non-toxic organic selenium and zinc, increasing the content of the organic selenium and zinc. The malts are subjected to beating, homogenizing, wall-breaking, double-enzyme hydrolyzing, centrifuging, impurity-removing, concentrating and drying to obtain the selenium-rich and zinc-rich protein peptide. The new method and new technology for obtaining plant nutrients is innovatively opened up.