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Extracting method for high-purity sweet potato protein

A sweet potato protein and extraction method technology, applied in the field of high-purity sweet potato protein extraction, can solve the problems of high impurity content, rapid protein flocculation, etc., and achieves the effects of process environmental protection, reduced extraction cost, and mild process conditions

Inactive Publication Date: 2015-08-05
QINGDAO HAIZHIXING BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many steps, and in the alkali extraction, acid extraction and organic solvent extraction of the centrifuge, alkali, acid and organic solvent are added to the centrifuge, resulting in rapid protein flocculation and high impurity content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for extracting high-purity sweet potato protein, the method comprises the following steps:

[0033] ①Wash and crush the sweet potatoes, pass through a 30-mesh sieve, add the sieved sweet potatoes to a constant temperature mixing tank, add pure water with a volume four times the volume of the crushed sweet potatoes to the mixing tank, and keep the temperature of the mixture in the mixing tank at 20~ Stir and soak for 16 hours at 25°C, 25-30°C, 30-35°C, and 35-40°C, and take the supernatant;

[0034] ②Centrifuge the supernatant obtained in step ① at 1000r / min for 15min, and take the supernatant;

[0035] ③The supernatant obtained in step ② is suction-filtered through a food-grade activated carbon layer, the suction filtration pressure is -0.08~-0.07Mpa, and the filtrate is taken;

[0036] ④ Add food-grade alcohol with a mass concentration of 80% and a volume of 2 to 4 times the volume of the filtrate obtained in step ③ into the constant temperature stirring tank...

Embodiment 2

[0046] A method for extracting high-purity sweet potato protein, the method comprises the following steps:

[0047] ①Wash and crush the sweet potatoes, pass through a 30-mesh sieve, put the sieved sweet potatoes into a constant temperature mixing tank, add pure water with a volume 4 times the volume of the crushed sweet potatoes to the mixing tank, and keep the temperature of the mixture in the mixing tank at 35~ Stir and soak at 40°C for 8h, 10h, 12h, 14h, and take the supernatant;

[0048] ②Centrifuge the supernatant obtained in step ① at 1000r / min for 15min in a centrifuge, and take the supernatant;

[0049] ③Filter the supernatant obtained in step ② through a food-grade activated carbon layer, the filter pressure is 0.4-0.6Mpa, and take the filtrate;

[0050]④ Add food-grade alcohol with a mass concentration of 80% and a volume twice the volume of the filtrate obtained in step ③ into a constant temperature stirring tank, start stirring, add the filtrate obtained in step ③...

Embodiment 3

[0057] A method for extracting high-purity sweet potato protein, the method comprises the following steps:

[0058] ①Wash and crush the sweet potatoes, pass through a 30-mesh sieve, put the sieved sweet potatoes into a constant temperature mixing tank, add pure water with a volume 4 times the volume of the crushed sweet potatoes to the mixing tank, and keep the temperature of the mixture in the mixing tank at 35~ Stir and soak at 40°C for 10 hours, and take the supernatant;

[0059] ②Centrifuge the supernatant obtained in step ① at 1000r / min for 15min in a centrifuge, and take the supernatant;

[0060] ③Filter the supernatant obtained in step ② through a food-grade activated carbon layer, the filter pressure is 0.4-0.6Mpa, and take the filtrate;

[0061] ④ Add food-grade alcohol with a mass concentration of 80% and a volume twice the volume of the filtrate obtained in step ③ into a constant temperature stirring tank, start stirring, add the filtrate obtained in step ③ into th...

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Abstract

The invention discloses an extracting method for high-purity sweet potato protein. The method comprises the following steps: (1) washing and crushing a sweet potato, sieving the crushed sweet potato, adding pure water, stirring and soaking, and taking supernatant; (2) centrifuging the supernatant obtained in the step (1), and taking the supernatant; (3) filtering or performing suction filtering on the supernatant obtained by the step (2) through a food-grade activated carbon layer; (4) slowly adding the filtrate obtained in the step (3) into food-grade alcohol under the stirring condition, and standing to obtain flocculent mixed liquid; (5) centrifuging the flocculent mixed liquid which is obtained in the step (4); (6) collecting the solid obtained in the step (5), and performing vacuum drying or freeze drying to obtain finished sweet potato protein. Compared with the conventional process, the extracting method for the sweet potato protein disclosed by the invention has the advantages that the process is simple and stable, conditions are mild, the environment-friendly effect is achieved, and the purity of the obtained sweet potato protein is 92 to 97 percent, and the purity is higher compared with the purity of the sweet potato protein of 72.4 percent obtained by the conventional process method.

Description

technical field [0001] The invention belongs to the field of protein extraction, in particular to a method for extracting high-purity sweet potato protein. Background technique [0002] Sweet potatoes are twining herbaceous vines, which are annual or perennial vines. Also known as sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, etc., they have different names due to different regions. Sweet potatoes are rich in mucus protein, which is a mixture of polysaccharides and proteins, which has a special protective effect on the human body and can maintain the lubrication of the digestive tract, respiratory tract, joint cavity, and membrane cavity and the elasticity of blood vessels. Arteriosclerosis caused by deposition on the arterial wall can prevent the atrophy of connective tissues in organs such as the liver and kidneys, slow down the aging of human organs, and improve the body's immunity. Sweet potato also contains glycoprot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00A23J1/16
Inventor 夏征梅
Owner QINGDAO HAIZHIXING BIOLOGICAL SCI & TECH
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