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Method for extracting tea protein product

An extraction method and tea protein technology, applied in the direction of protein composition of microorganisms, protein components of waste, protein components of plant materials, etc., can solve problems such as ultrasound-assisted extraction methods for tea protein products that have not been seen, and achieve improved protein production The effect of purity, high efficiency and low energy consumption

Inactive Publication Date: 2012-01-04
中华全国供销合作总社杭州茶叶研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to new research, there is no research report or patent publication on the ultrasonic-assisted extraction method of tea protein products at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 ﹑A method for extracting tea protein products, the steps are:

[0023] (1) The tea leaves are washed with 0.01% EDTA-2Na aqueous solution and 35% ethanol solution to remove dust, metal ions and some phenolic substances in the raw materials, drained and dried, and the dried raw materials have a water content of 8%;

[0024] (2) Tea leaves are crushed and sieved to 100 mesh;

[0025] (3) Low-frequency cyclic ultrasonic-assisted extraction of tea protein: Weigh 1kg of raw materials, add 20 times the weight of alkaline solution with a pH of 10 to it, and use low-frequency cyclic ultrasonic-assisted extraction technology to extract the active ingredients such as protein, wherein the ultrasonic auxiliary conditions are : Ultrasonic power: 300W, ultrasonic frequency: 24.5Hz; extraction conditions: material liquid temperature 40°C, extraction time 40min; after the extraction is completed, centrifuge at 3000r / min for 40min, discard the residue, and obtain the protein ...

Embodiment 2

[0031] Example 2 ﹑A method for extracting tea protein products, the steps are

[0032] (1) Wash the tea leaves with 0.02% EDTA-2Na aqueous solution and 50% ethanol solution to remove dust, metal ions and some phenolic substances in the raw materials, drain and dry;

[0033] (2) Tea leaves are crushed and sieved to 150 mesh;

[0034](3) Low-frequency cyclic ultrasonic-assisted extraction of tea protein: Weigh 1 kg of raw materials, add 30 times the weight of an alkaline solution with a pH of 12 to it, and use low-frequency cyclic ultrasonic-assisted extraction technology to extract the active ingredients such as protein, wherein the ultrasonic auxiliary conditions are : Ultrasonic power: 250W, ultrasonic frequency: 26.5Hz; extraction conditions: material liquid temperature 55°C, extraction time 60min; after extraction, centrifuge at 6000r / min for 20min, discard the residue, and obtain protein extract;

[0035] (4) Decolorize the protein extract: add 4% hydrogen peroxide solu...

Embodiment 3

[0040] Example 3 ﹑A method for extracting tea protein products, the steps are:

[0041] (1) Wash the tea residue with 0.03% EDTA-2Na aqueous solution and 75% ethanol solution to remove dust, metal ions and some phenolic substances in the raw material, drain and dry;

[0042] (2) Tea leaves are crushed and sieved to 200 mesh;

[0043] (3) Low-frequency cyclic ultrasonic-assisted extraction of tea protein: Weigh 1 kg of raw materials, add 40 times the weight of alkaline solution with a pH of 13 to it, and use low-frequency cyclic ultrasonic-assisted extraction technology to extract the active ingredients such as protein, wherein the ultrasonic auxiliary conditions are : Ultrasonic power: 300W, ultrasonic frequency: 23.5Hz; extraction conditions: feed liquid temperature 80°C, extraction time 90min; after extraction, centrifuge at 10000r / min for 20min, discard the residue to obtain protein extract;

[0044] (4) Decolorize the protein extract: add 7% hydrogen peroxide solution t...

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Abstract

The invention provides a method for extracting a tea protein product. The method specifically comprises the following steps of: 1) pretreating raw materials; 2) preparing tea protein through ultrasonic-assisted alkali extraction; 3) decolorizing a tea protein solution; 4) concentrating a tea protein extracting solution; 5) precipitating the tea protein; and 6) washing the tea protein, refining and drying. The method has the advantages that: tea residue or low-grade tea such as summer-autumn tea and the like is used as a raw material, and a low-frequency ultrasonic circulation technology is used for assisting in extracting the tea protein; by the method, the protein is high in extraction rate and has high purity; the method is low in cost, simple and practicable, and easy to popularize; and the obtained tea protein product has green tea color, and the quality of the product meets food hygienic standard. A novel way of value-added transformation and recycling of tea residue and summer-autumn low-grade tea is provided; and a novel botanical protein product is developed, and the comprehensive utilization and fine and deep processing of tea resources are realized.

Description

technical field [0001] The invention relates to the field of development and utilization of plant products, and mainly relates to a method for extracting tea protein products. Background technique [0002] my country is a big country of tea production, the tea industry is developing rapidly, the area and output of tea gardens are constantly increasing, and there is a trend of oversupply of tea resources. According to FAO-IGG statistics, the global tea production in 2009 reached 3.9284 million tons. In the past 10 years, China's tea output has continued to grow. From 2000 to 2007, the tea output increased from 719,300 tons to 1.14 million tons, with an average annual growth rate of nearly 11%. In 2009, it reached 1.31 million tons, making it the largest tea producer in the world. country. The consumption of tea increased from 456,000 tons in 2000 to 660,000 tons in 2006, with an average annual growth rate of less than 6%. Analyzing the reasons, on the one hand, due to the ...

Claims

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Application Information

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IPC IPC(8): A23J1/00
Inventor 张士康朱科学陆晨周惠明王彬彭伟蒋玉兰
Owner 中华全国供销合作总社杭州茶叶研究院
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