Method for extracting tea protein product
An extraction method and tea protein technology, applied in the direction of protein composition of microorganisms, protein components of waste, protein components of plant materials, etc., can solve problems such as ultrasound-assisted extraction methods for tea protein products that have not been seen, and achieve improved protein production The effect of purity, high efficiency and low energy consumption
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Embodiment 1
[0022] Example 1 ﹑A method for extracting tea protein products, the steps are:
[0023] (1) The tea leaves are washed with 0.01% EDTA-2Na aqueous solution and 35% ethanol solution to remove dust, metal ions and some phenolic substances in the raw materials, drained and dried, and the dried raw materials have a water content of 8%;
[0024] (2) Tea leaves are crushed and sieved to 100 mesh;
[0025] (3) Low-frequency cyclic ultrasonic-assisted extraction of tea protein: Weigh 1kg of raw materials, add 20 times the weight of alkaline solution with a pH of 10 to it, and use low-frequency cyclic ultrasonic-assisted extraction technology to extract the active ingredients such as protein, wherein the ultrasonic auxiliary conditions are : Ultrasonic power: 300W, ultrasonic frequency: 24.5Hz; extraction conditions: material liquid temperature 40°C, extraction time 40min; after the extraction is completed, centrifuge at 3000r / min for 40min, discard the residue, and obtain the protein ...
Embodiment 2
[0031] Example 2 ﹑A method for extracting tea protein products, the steps are
[0032] (1) Wash the tea leaves with 0.02% EDTA-2Na aqueous solution and 50% ethanol solution to remove dust, metal ions and some phenolic substances in the raw materials, drain and dry;
[0033] (2) Tea leaves are crushed and sieved to 150 mesh;
[0034](3) Low-frequency cyclic ultrasonic-assisted extraction of tea protein: Weigh 1 kg of raw materials, add 30 times the weight of an alkaline solution with a pH of 12 to it, and use low-frequency cyclic ultrasonic-assisted extraction technology to extract the active ingredients such as protein, wherein the ultrasonic auxiliary conditions are : Ultrasonic power: 250W, ultrasonic frequency: 26.5Hz; extraction conditions: material liquid temperature 55°C, extraction time 60min; after extraction, centrifuge at 6000r / min for 20min, discard the residue, and obtain protein extract;
[0035] (4) Decolorize the protein extract: add 4% hydrogen peroxide solu...
Embodiment 3
[0040] Example 3 ﹑A method for extracting tea protein products, the steps are:
[0041] (1) Wash the tea residue with 0.03% EDTA-2Na aqueous solution and 75% ethanol solution to remove dust, metal ions and some phenolic substances in the raw material, drain and dry;
[0042] (2) Tea leaves are crushed and sieved to 200 mesh;
[0043] (3) Low-frequency cyclic ultrasonic-assisted extraction of tea protein: Weigh 1 kg of raw materials, add 40 times the weight of alkaline solution with a pH of 13 to it, and use low-frequency cyclic ultrasonic-assisted extraction technology to extract the active ingredients such as protein, wherein the ultrasonic auxiliary conditions are : Ultrasonic power: 300W, ultrasonic frequency: 23.5Hz; extraction conditions: feed liquid temperature 80°C, extraction time 90min; after extraction, centrifuge at 10000r / min for 20min, discard the residue to obtain protein extract;
[0044] (4) Decolorize the protein extract: add 7% hydrogen peroxide solution t...
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