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Process for preparing fly larva albumen powder by enzymatic hydrolysis of fly larvae

A technology of protein powder and enzymatic hydrolysis, applied in the direction of protein food components, protein components of waste, protein composition of microorganisms, etc., can solve the problems of large nutritional loss of protein, complex products, complex protein raw materials, etc., and achieve the goal of increasing the degree of hydrolysis Effect

Inactive Publication Date: 2014-12-17
GUILIN HEYINXIANG NEW MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Protein hydrolysis methods mainly include acid, alkali hydrolysis and enzymatic hydrolysis. Among them, the products of acid and alkali hydrolysis are complex, and the nutritional loss of protein is relatively large. Therefore, enzymatic hydrolysis of fly maggots is often used to obtain a large amount of active peptides, so that the fly maggots A variety of effective substances are fully utilized; however, due to the different specificities of various proteases and the complexity of protein raw materials, compound enzyme methods are often used for hydrolysis of fly maggots

Method used

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  • Process for preparing fly larva albumen powder by enzymatic hydrolysis of fly larvae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Get fresh fly maggot, add and be equivalent to its weight 10 times of water refining, adjust its pH to be 6.5, then add the enzyme composition that is equivalent to fresh fly maggot weight 3% (the weight ratio of composite protease, neutral protease and flavor protease is 1:1:0.5), heat up to 50°C and keep enzymatic hydrolysis for 5 hours, inactivate the enzyme, filter, collect the filtrate (the degree of hydrolysis is 61%), concentrate under reduced pressure, and spray dry to obtain the fly maggot protein powder.

[0014] The obtained fly maggot protein powder is added with water to form a solution with a mass concentration of 5%, which has no bitter taste and a little fishy smell.

[0015] The detection method of the degree of hydrolysis of the sample in this embodiment is: take 5 mL of the filtrate collected by filtration after inactivating the enzyme and set the volume to 100 mL, and use the ninhydrin method to measure its amino acid content, and the degree of incomp...

Embodiment 2

[0018] Get fresh fly maggot, add and be equivalent to its weight 5 times of water refining, adjust its pH to be 7.0, then add the enzyme composition that is equivalent to fresh fly maggot weight 5% (the weight ratio of compound protease, neutral protease and flavor protease is 1:1.5:1), heat up to 45°C and keep enzymatic hydrolysis for 4 hours, inactivate the enzyme, filter, collect the filtrate (the degree of hydrolysis is 58%, the detection method is the same as in Example 1), concentrate under reduced pressure, and spray dry to obtain the fly maggot protein pink.

[0019] The obtained fly maggot protein powder is added with water to form a solution with a mass concentration of 5%, which has no bitter taste and a little fishy smell.

Embodiment 3

[0021] Get fresh fly maggot, add and be equivalent to its weight 8 times of water refining, adjust its pH to be 6.0, then add the enzyme composition that is equivalent to fresh fly maggot weight 2% (the weight ratio of composite protease, neutral protease and flavor protease is 1:1.5:0.8), heat up to 55°C and heat for 7 hours, inactivate the enzyme, filter, collect the filtrate (the degree of hydrolysis is 62%, the detection method is the same as in Example 1), concentrate under reduced pressure, and spray dry to obtain the fly maggot protein pink.

[0022] The obtained fly maggot protein powder is added with water to form a solution with a mass concentration of 5%, which has no bitter taste and a little fishy smell.

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Abstract

The invention discloses a process for preparing fly larva albumen powder by enzymatic hydrolysis of fly larvae. The process comprises the following specific steps: adding water to fresh fly larvae, pulping, and adjusting the pH as 6.0-7.0; adding an enzyme composition, raising the temperature to 45-55 DEG C, performing heat-preservation enzymatic hydrolysis for 4-7h, and performing enzyme deactivation, filtration, filtrate collection, concentration and drying to obtain the fly larva albumen powder, wherein the enzyme composition is formed by compound protease, neutral protease and flavourzyme according to the weight ratio of 1:(1-2):(0.5-1), and the addition amount of the enzyme composition is 2-5 weight percent of the fresh fly larvae. Compared with the prior art, the process has the advantages that the enzyme composition formed by the compound protease, the neutral protease and the flavourzyme according to a specific ratio performs enzymatic hydrolysis on the pulp of the fresh fly larvae under specific conditions, all enzymes cooperate in the enzymatic hydrolysis process, the degree of hydrolysis is effectively improved, and meanwhile, the prepared fly larva albumen powder does not taste bitter, and only has slightly fishy smell.

Description

technical field [0001] The invention relates to a process for preparing fly maggot protein powder by enzymatically hydrolyzing fly maggots, and belongs to the technical field of natural product extraction. Background technique [0002] Fly maggot is a high-quality animal protein source. It is not only rich in protein content, but also has a reasonable and complete amino acid composition. According to literature reports, crude protein accounts for 55-65% of fly maggot dry powder. [0003] Protein hydrolyzate is a mixture of oligopeptides, small molecular peptides and amino acids, which shows broad application prospects due to its superior processing performance and nutritional properties. Protein hydrolysis methods mainly include acid, alkali hydrolysis and enzymatic hydrolysis. Among them, the products of acid and alkali hydrolysis are complex, and the nutritional loss of protein is relatively large. Therefore, enzymatic hydrolysis of fly maggots is often used to obtain a la...

Claims

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Application Information

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IPC IPC(8): A23J1/00
Inventor 周冠旻
Owner GUILIN HEYINXIANG NEW MATERIALS
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