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Tea flower protein extract and application thereof

A technology of protein extract and tea tree flower, which is applied to the protein composition of microorganisms, protein components of waste, protein components of plant materials, etc., can solve the problems of little research, achieve excellent water holding capacity, high extraction rate, and realize The effect of effective use

Active Publication Date: 2014-12-10
杭州英仕利生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effective use of tea tree flower resources has a good effect on increasing the economic value of the tea industry chain, but there is little research on tea tree resources other than tea resources
There is no research report on the preparation of tea tree flower functional protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A kind of tea tree flower protein extract, preparation method comprises the following steps:

[0040] (1) Take the tea tree flowers in the blooming stage, dry them at a constant temperature of 70°C (moisture content is controlled within 8%), and grind them to 60 mesh to obtain the tea tree flower dry powder;

[0041] (2) Take 100g of tea tree flower dry powder, use NaOH solution with a concentration of 0.05mol / L as the extraction solution, the mixing ratio of tea tree flower dry powder and NaOH solution (hereinafter referred to as the solid-liquid ratio) is 1:10, and place it in a water bath at 45°C Leach for 0.5h to obtain the extract;

[0042] Or, get tea tree flower dry powder 100g, be the alkaline protease solution of 1% with alkaline protease addition as extracting liquid (pH≈9), the mixing ratio (hereinafter referred to as solid-to-liquid ratio) of tea tree flower dry powder and alkaline protease solution is 1:30, placed in a water bath at 40°C for 1 hour;

[00...

Embodiment 2

[0047] A kind of tea tree flower protein extract, preparation method comprises the following steps:

[0048] (1) Take tea tree flowers in the dew-white stage, dry them at a constant temperature of 70°C (moisture content is controlled within 8%), and pulverize them to 60 meshes to obtain dry tea tree flowers powder;

[0049] (2) Take 100 g of tea tree flower dry powder, use a NaOH solution with a concentration of 0.25 mol / L as the extraction solution, and have a solid-to-liquid ratio of 1:20, place it in a water bath at 80°C for 1.5 hours, and obtain the extraction solution;

[0050] Alternatively, take 100 g of tea tree flower dry powder, use an alkaline protease solution with an added amount of alkaline protease of 3% as the extraction solution (pH≈9), and have a solid-to-liquid ratio of 1:40, and place it in a water bath at 60°C for 3 hours;

[0051] (3) Add 10% (weight volume fraction, g / ml) of activated carbon to the extract, decolorize at 50° C. for 1 hour, and filter the...

Embodiment 3

[0055] A kind of tea tree flower protein extract, preparation method comprises the following steps:

[0056] (1) Take the tea tree flower at the early blooming stage, dry it at a constant temperature of 70°C (moisture content is controlled within 8%), and grind it to 60 mesh to obtain the tea tree flower dry powder;

[0057] (2) Take 100 g of tea tree flower dry powder, use NaOH solution with a concentration of 0.10 mol / L as the extraction solution, and the solid-to-liquid ratio is 1:30, place it in a water bath at 60°C for 2.5 hours, and obtain the extraction solution;

[0058] Alternatively, take 100 g of tea tree flower dry powder, use an alkaline protease solution with an added amount of alkaline protease of 5% as the extraction solution (pH≈9), and have a solid-to-liquid ratio of 1:40, and place it in a water bath at 50°C for 1 hour;

[0059] (3) Add 8% (weight volume fraction, g / mL) of activated carbon to the extract, decolorize at 50° C. for 1 hour, and filter the filtr...

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Abstract

The invention discloses a tea flower protein extract and an application thereof. The preparation method of the tea flower protein extract comprises the following steps: (1) drying and grinding tea flowers to obtain tea flower powder; (2) extracting the tea flower powder by using an alkaline method or an enzyme method, and taking the extract liquid; and (3) decoloring the extract liquid, adjusting the pH value to ensure that the protein precipitates and finally separating to obtain the tea flower protein extract. The tea flower extract is obtained through alkaline method extraction and enzyme method extraction, the extraction rate of the alkaline method is up to 91.45% and the extraction rate of the enzyme method is up to 79.12%, so that both the extraction rates are relatively high and the effective utilization of the tea flower resource is realized. Compared with the tea leaf protein, the tea flower protein extract has excellent water retention capacity, oil absorbency, emulsifying property, foaming characteristic and antioxidation, and can be used for preparing antioxidants, foaming agents and emulsifiers with better performance.

Description

technical field [0001] The invention relates to the technical field of tea tree flower development and utilization, in particular to a tea tree flower protein extract and its application. Background technique [0002] Tea tree (scientific name: Camellia sinensis), belonging to the family Theaceae Camellia, is a perennial evergreen woody plant. It is generally a shrub, and there are arbor-type tea trees as high as 15 to 30 meters in the tropics, and the circumference of the base tree is more than 1.5 meters. Cultivated tea trees often suppress longitudinal growth by pruning, so the tree height is mostly between 0.8 and 1.2 meters. The leaves of the tea tree are elliptical with serrations on the edges, and five petals of white flowers are opened between the leaves. The fruit is oblate and triangular in shape, and the seeds are exposed after the fruit cracks. [0003] my country is a big country of tea production, the tea industry is developing rapidly, the area and output of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00
Inventor 吴媛媛侯玲屠幼英朱羚玮陈琳
Owner 杭州英仕利生物科技有限公司
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