Method and device for preparing bacterial cellulose composite material quickly on large scale

A technology of bacterial cellulose and composite materials, applied in biochemical equipment and methods, methods based on microorganisms, biochemical cleaning devices, etc., can solve the problems of not covering most of the gaps, long cultivation period, uneven distribution, etc., and achieve The effect of shortening the production cycle, long cycle, and cumbersome process

Active Publication Date: 2012-07-04
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing preparation of bacterial cellulose membrane composite materials is based on static technology, including placing the materials that need to be composited with bacterial cellulose in the culture solution for co-cultivation, or doping the bacterial cellulose membrane with organic polymers or inorganic substances by impregnation. Composite, the efficiency is not high; because the cellulose is only formed on the surface of the medium, the cellulose composited by the standing method is not only small, but also unevenly distributed, and most of the gaps of the materials to be composited cannot be covered. In addition, the cultivation period is very long

Method used

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  • Method and device for preparing bacterial cellulose composite material quickly on large scale
  • Method and device for preparing bacterial cellulose composite material quickly on large scale
  • Method and device for preparing bacterial cellulose composite material quickly on large scale

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Bacteria culture

[0053] Insert Acetobacter xylinum into 300mL liquid medium (per 1L of water, 20g of mannitol, 3g of peptone, 5g of yeast extract, pH 3.0, sterilized at 121°C for 20min; or 200g of glucose, 5g of yeast extract, Tryptone 5g, citric acid 115g, Na 2 HPO 4 2.7g, 1L of water, pH 7.5, sterilized at 121°C for 20min) for expansion, cultured on a shaking table at 20°C, 100r / min or standing for 12h before use;

[0054] (2) Fermentation preparation of bacterial cellulose composite membrane

[0055] The liquid culture medium containing the production strain prepared in step (1) is transferred to a bioreactor equipped with a fixed gauze or silk skeleton material drum, and then the drum rotates at a speed of 7, 15, 30 and 60rpm for disturbance cultivation , after dynamic culture at 30°C for 1 day, the bacterial cellulose / gauze or bacterial cellulose / silk composite film can be obtained, and the film formation conditions are shown in Table 1; On the skeleton m...

Embodiment 2

[0066] (1) Bacteria culture

[0067] Put kombucha (kombucha) into 300mL liquid seed culture medium according to the inoculation amount of 1-10 discs with a diameter of 0.5cm containing bacteria BC membrane (per 1L of water, green tea 5g, glucose 10, peptone 3g, yeast extract 5g , pH3.0, pasteurized for 30min; per 1L of water, 100g of glucose, 3g of peptone, 5g of yeast extract, pH7.5, sterilized at 121°C for 20min) to expand the culture, shake the table at 25°C and 150r / min Cultivate or statically cultivate for 24 hours before use;

[0068] (2) Fermentation preparation of bacterial cellulose composite membrane

[0069] The liquid medium containing the production strain prepared in step (1) is transferred to a bioreactor equipped with a drum of polyester, nylon or polypropylene fiber skeleton material, and then the rotating drum is disturbed and cultivated at a speed of 15 or 25rpm. After 10 days of dynamic culture at 30°C, the film-forming product can be harvested;

[0070]...

Embodiment 3

[0076] (1) Bacteria culture

[0077] Insert Gluconacetobacter xylinus into 300mL liquid medium (per 1L of water, maltose 100g, peptone 3g, yeast extract 5g, pH 4.5, sterilized at 121°C for 20min; or fructose 160g, yeast extract 5g, tryptone 5g, citric acid 1.15g, Na 2 HPO 4 2.7g, 1L of water, pH 6.0, sterilized at 121°C for 20min) for expansion, cultured on a shaking table at 30°C, 250r / min or standing for 48h before use;

[0078] (2) Fermentation preparation of bacterial cellulose composite membrane

[0079] Transfer the liquid culture medium containing the production strain prepared in step (1) to a bioreactor equipped with a titanium dioxide metal mesh drum, then rotate the drum at a speed of 15 rpm for disturbed culture, and culture at 30°C for 24h and 36h respectively After 48h and 60h, the bacterial cellulose / titanium dioxide composite membrane can be obtained;

[0080] Or get the activated bacterial classification of step (1) to insert in the 300mL liquid fermentati...

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Abstract

The invention relates to a method and device for preparing bacterial cellulose composite materials quickly on a large scale. The method comprises the steps as follows: (1) a bacterial cellulose producing strain is inoculated in liquid medium for propagation and then transferred into a biological reactor containing framework material for perturbation cultivation, so that bacterial cellulose composite material can be obtained; and (2) bacterial cellulose of the bacterial cellulose composite material is peeled off from the framework material or the cellulose composite material is soaked in NaOH solution to be washed. The device comprises a thermometer opening, an acid liquor and alkali liquor feeding opening, a handle, an air vent, a pH meter opening, a nutrition feeding opening, a rotating shaft, a fermentation tank, a motor, a rotating drum, a fermentation tank jacket, a circulating water inlet, a circulating water outlet, a nutrient fluid inlet, a nutrient fluid outlet and a condensed water outlet. The rotating drum is fixed on the rotating shaft in the fermentation tank. The device provided by the invention has the advantages of simplicity, convenience, detachable die, low cost, high production efficiency and high degree of automation; and the obtained bacterial cellulose composite material can be widely applied.

Description

technical field [0001] The invention belongs to the field of preparation of bacterial cellulose materials and devices thereof, and in particular relates to a method and a device for rapid and large-scale preparation of bacterial cellulose composite materials. Background technique [0002] Bacterial Cellulose (BC) is a kind of pure cellulose produced by microorganisms, which can be widely used in food, facial masks, high-strength functional materials, textiles, papermaking, artificial blood vessels, tissue engineering scaffolds, artificial skin and medical dressings, etc. field. However, the existing shallow plate fermentation production equipment has low production efficiency and output, long production cycle, high production cost, low degree of mechanization, high labor intensity, and cannot be continuously produced. Therefore urgently need to develop a kind of output high, and the novel fermentation device that can efficient continuous production. [0003] Wet pure bacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12M1/00C12R1/01C12R1/02C12R1/41C12R1/38C12R1/025C12R1/05C12R1/065
CPCC12M41/12C12M41/22C12M27/06
Inventor 洪枫唐水佳杨雪霞
Owner DONGHUA UNIV
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